LIF-Dependent Porcine iPSCs
|T2503||1x106 cells / 1.0 ml|
|Description||Leukemia inhibitory factor-dependent induced pluripotent stem cells were derived from the inner cell mass of porcine blastocysts by up-regulating expression of KLF4 and POU5F1. They are dependent on leukemia inhibitory factor signaling for maintenance of pluripotency; can be cultured over extended passage; and have the ability to form teratomas. Resulting colonies resemble mouse embryonic stem cells in terms of colony morphology; cell cycle interval; transcriptome; and the expression of pluripotent markers. This cell line was created by lentiviral transduction with hKLF4 and M2rtTA virus.|
|Applications||For Research Use Only|
|Seeding Density||10,000 - 20,000 cells/cm2|
|Cell Type||Ready-to-Use Stem Cells|
|Expression Profile||Pluripotent markers; such as POU5F1; SOX2; and surface marker SSEA1.|
|Subculture Protocol||1:10 split; Accutase (Millipore) used|
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Reverse transcriptase PCR; 2) Real time PCR; 3) Transcriptional profiling by microarray; 4) Alkaline phosphatase staining; 5) Immunofluorescence; 6) Western blot; 7) Karyotyping|
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