MfeI

CAT.NOUNITPRICE
E05710000 U (500 µl)
$320.00

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MfeI
MfeI

In stock

$320.00

Summary
    Specifications


    SKUE057
    Unit quantity10000 U (500 µl)
    Applications

    Restriction enzyme digestion of double-stranded DNA

    Components

    MfeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

    Concentration20000 U/ml
    Expression SystemAn E.coli strain that carries the modified MfeI gene from Mycoplasma fermentas (N.F. Halden). The MfeI enzyme recognizes and cuts the sequence C^AATTG.
    Inactivation ProtocolNo
    Reaction Definition1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204). Incubate at 37°C
    Reaction Buffer

    Buffer 1: 75%, Buffer 2: 25%, Buffer 3: <10%, Universal Buffer: 100%
    Click here to view the buffer compatibility chart for restriction enzymes.

    Storage Condition

    -20°C

    Storage Buffer10mM Tris pH (7.5), 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol.
    Note

    Methylation Sensitivity
    dam methylation: Not Sensitive
    dcm methylation: Not Sensitive
    CpG Methylation: Blocked by Some Combinations of Overlapping

    Documents


    Supporting Protocol
    MSDS

      QC
      Other

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