miRNA cDNA Synthesis Kit

The miRNA cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand miRNA from total RNA templates. The kit utilizes a mutated recombinant M-MuLV Reverse Transcriptase which exhibits limited RNase H activity. The recombinant RNasin™ Ribonuclease Inhibitor, supplied with the kit effectively protects the RNA template from degradation. Besides the basic miRNA cDNA synthesis kit (G269/G270), customer also has the option to choose a miRNA cDNA synthesis kit with Poly(A) Polymerase Tailing (G902/G903). Both versions of abm’s miRNA cDNA synthesis kits come along with oligo d(T) adapter, which anneals selectively onto the poly(A)-tailed miRNA. Universal reverse primers, included in miRNA profiling kit (MA003, MA004), contains the adaptor sequence and therefore they must be used on the cDNA generated by abm’s miRNA cDNA synthesis kit. The first strand of miRNA’s cDNA can be directly used as a template for the qPCR-based analysis of miRNA expression in a given sample. abm also has a complete library of miRNA primers for all the known miRNA sequences; abm is one of the very few companies in the world that provide industry-leading-quality miRNA profiling services with years of experience. All our primers have been extensively optimized for qPCR conditions and have been validated for their accuracy.