miRNA cDNA Synthesis Kit

The miRNA cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand miRNA from total RNA templates. The kit utilizes a mutated recombinant M-MuLV Reverse Transcriptase which exhibits limited RNase H activity. The recombinant RNasin™ Ribonuclease Inhibitor, supplied with the kit effectively protects the RNA template from degradation. Besides the basic miRNA cDNA synthesis kit (G269/G270), customers also have the option to choose a miRNA cDNA synthesis kit with Poly(A) Polymerase Tailing (G902/G903). Both versions of abm's miRNA cDNA synthesis kits come along with oligo d(T) adapter, which anneals selectively onto the poly(A)-tailed miRNA. The first strand of miRNA's cDNA can be directly used as a template for the qPCR-based analysis of miRNA expression in a given sample. abm also has a complete library of miRNA primers for all the known miRNA sequences. All our primers have been extensively optimized for qPCR conditions and have been validated for their accuracy.