HotStart DNA Polymerase Kits

Problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, abm’s HotStart DNA Polymerase offers improved yield of desired PCR products.

With abm’s HotStart DNA Polymerase, you can achieve 
• The highest specificity with minimal background
• Superior performance
• Improved yield of desired product

HotStart  DNA Polymerase is offered in various formats:

• Enzyme only (Cat. No. G011 and G039)
• 2X MasterMix (Cat. No. G906) to help streamline the PCR workflow. 
• 2X MasterMix with dye (Cat. No. G906-Dye) to allow visualization on gel electrophoresis. Our 2X PCR HotStart MasterMix with Dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.

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  1. Price: $115.00
    Unit: 5.0 ml (200 Rxns)
    Problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation...


  2. Price: $115.00
    Unit: 5.0 ml (200 Rxns)
    Problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation...


  3. Price: $115.00
    Unit: 1,000 U (200 µl)
    Problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation...


  4. Price: $35.00
    Unit: 250 U (50 µl)
    Problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation...

4 Items

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