BrightGreen qPCR MasterMix Kits

Guaranteed high-performance real-time PCR using abm’s BrightGreen 2X qPCR MasterMix. abm’s BrightGreen 2X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Available with the option of ROX or fluorescein as the internal passive reference dye, abm’s BrightGreen 2X qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers.

Key Features

  • Antibody hot-start technology provides superior specificity
  • Enable streamlined protocol in a simple reaction set-up
  • Allow accurate quantification of a variety of gene targets
  • Reduce pipetting steps to minimize the risk of contamination
  • Compatible with most real-time PCR instruments

Please refer to our qPCR MasterMix Selection Guide under Document to select the appropriate qPCR formulation applicable to your particular instrument model.

Key Features 1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles. 2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results. 3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range. 4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is less than 150 bp.

This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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