ExCellenCT One-Step TaqProbe qRT-PCR Kits

abm's ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be applied in One-Step qRT-PCR.

Convenient real-time RNA quantification in one EASY step. abm's One-Step TaqProbe qRT-PCR Kit uses a combination of high-quality enzymes in a proprietary buffer system to deliver precise and accurate sample analysis for high-throughput applications. This kit offers ultimate convenience in addition to consistent performance in terms of high sensitivity and superb signal-to noise ratio. 

Key Features 
Streamlined protocol in a simple, single-tube reaction set-up 
High-quality, full-length cDNA from as little as 0.01pg of RNA template 
Fully optimized for detection of low-copy genes 
Simple set-up for any RNA template (e.g. mRNA and total RNA) 
Reduces pipetting steps to minimize the risk of contamination and error. 
Improved fidelity and yield for reverse transcription 
Prevention of template (RNA) degradation with RNaseOFF Ribonuclease Inhibitor. 
Superb performance with respect to sensitivity and signal-to-noise ratio. 
Significant reduction in non-specific PCR amplification by utilizing HotStart Taq DNA polymerase in the enzyme mix. 

Please refer to our qPCR MasterMix Selection Guide under Document to select the appropriate qPCR formulation applicable to your particular instrument model. This kit includes 50X qRT-PCR Enzyme Mix and TaqProbe qPCR MasterMix

Notes: 
1. Aliquot reagents to avoid risk of contamination and repeated freeze-thaw cycles. 
2. Avoid high primer concentrations; a concentration of 100 nM to 300 nM of each primer usually gives the best results. 
3. Probe concentration may require optimization, typically a 3:1 to 1:1 primer:probe ratio will give good amplification. 
4. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: 1) preparing amplicon specific pre-mix, 2) using repeating pipettes and 3) keeping the pipetting volume within the manufacturer's suggested range. 
5. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp. 

This product is distributed for laboratory research only. 
Caution: Not for diagnostic use.

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  1. Price: $135.00
    Unit: 25 Preps, 100 x 20 µl reactions
    Extracting and preparing RNA templates directly from cultured cells to be applied as template for One-Step qRT-PCR, with G493 One-Step TaqProbe qRT-PCR MasterMix.abm’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide...


  2. Price: $135.00
    Unit: 25 Preps, 100 x 20 µl reactions
    Extracting and preparing RNA templates directly from cultured cells to be applied as template for One-Step qRT-PCR, with G493 One-Step TaqProbe qRT-PCR MasterMix.abm’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide...


  3. Price: $135.00
    Unit: 25 Preps, 100 x 20 µl reactions
    Extracting and preparing RNA templates directly from cultured cells to be applied as template for One-Step qRT-PCR, with G493 One-Step TaqProbe qRT-PCR MasterMix.abm’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide...


  4. Price: $135.00
    Unit: 25 Preps, 100 x 20 µl reactions
    Extracting and preparing RNA templates directly from cultured cells to be applied as template for One-Step qRT-PCR, with G493 One-Step TaqProbe qRT-PCR MasterMix.abm’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide...

4 Items

per page
Set Descending Direction