Mouse Rad9 -/- Embryonic Stem Cells
|T2510||1x106 cells / 1.0 ml|
|Description||Rad9; a checkpoint gene; plays a key role in the regulation of cellular response to DNA damage and the maintenance of genomic integrity. Mouse Rad9 -/- Embryonic Stem Cells were developed using a Cre/Loxp conditional knockout system. This cell line is suitable for research regarding the functions of Rad9; including the effect of loss of function in tumorigenesis.|
|Species||Mouse (M. musculus)|
|Applications||For Research Use Only|
|Cell Type||Ready-to-Use Stem Cells|
|Expression Profile||Mrad9 -/- ES cells were transfected with pZeoSV2-Mrad9 and pZeoSV2-HRAD9 and then challenged with zeocin|
|Subculture Protocol||Cells should be kept at a density such that they can be split every second day at 1:6 dilution. Change medium between splits.|
|Preservation Protocol||Freezing medium contains D-MEM with 25% FBS and 10% DMSO.|
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Southern blot; 2) PCR|
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