pLenti-UTR-Dual-Luciferase Blank Vector
Control lentiviral vector expressing firefly luciferase and renilla luciferase.
|Unit quantity||1.0 μg|
1 year when stored at -20°C or lower in a non-frost free freezer.
- Lentiviral Vector Amplification Protocol
- Selection-Drug Killing Curve
- Lentivirus Packing Protocol (for use with Vectors only)
- Lentivirus Infection Protocol
- UTR Reporters Handbook
- Enhanced Lentivirus Safety Features: Replication Incompetency
- Suggested MOI for Common Cancer Cell Lines
|I am very interested in your miRNA products. How about the price for the 3' UTR Luciferase/GFP Reporters? Could I directly order with your company or online and pay by PO?|
To search for the price of 3'UTR product, please go to http://www.abmgood.com/microRNA-miRNA/targetvalid.php?csn=26&ssn=11838&dsn=12288, and enter the gene name. You can place your order by email, telephone, fax, mail or online. PO number or Visa can be used. Please see the detail by clicking the following link http://www.abmgood.com/misc/orders.php
|What is the source of the Luciferase gene?|
It is from the firefly.
|How does the 3'UTR platform work?|
The stable cell line or the infected cells from the lentivirus will consistently express luciferase/GFP unless there are miRNA that activate the 3'UTR to "knockdown" the reporter expression.
|What is the forward and reverse sequencing primers for the 3'UTR luciferase reporter systems?|
Forward sequencing primer: Luc Forward primer 5'-GCAAGTTGGACGCCCGCAAGATC-3' Reverse sequencing primer: SV40 promoter reverse primer 5`-TAGTCAGCCATGGGGCGGAGA -3'
|Whats the parental cell line for the 3'UTR stable cells?|
|What is the upper size limit for the 3'UTR insert for viral packaging?|
For GFP, we can fit a 3.5kb insert for efficient packaging. For Luc, we can fit a 2.6kb insert for efficient packaging. The above size limits can be stretched a little (~0.5kb-1kb larger), but the packaging efficiency and virus titer will be lower.
|Could 3'UTR GFP reporter be used with a conventional transient transfection system (e.s.Lipofectamin 2000)? Could this vector be used to validate the activity of an endogenous overexpressed miRNA?|
Yes, this vector can also be transiently transfected using a transfection reagent such as lipofectamine 2000, or DNAfectin available from abm. This reporter construct can also be used to validate interactions of an endogenously expressed miRNA, if the known target site is present in the 3' UTR sequence.