Poly(A) Polymerase, Yeast

E017100 U (100 μl)

Customize Poly(A) Polymerase, Yeast
1 x PolyA Polymerase, Yeast   + $0.00
1 x 5X Reaction Buffer   + $0.00
1 x 25mM MnCl2   + $0.00
1 x ATP   + $0.00

* Required Fields

Your Customization
Poly(A) Polymerase, Yeast
Poly(A) Polymerase, Yeast

In stock




    Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.

    Unit quantity100 U (100 μl)

    • Labelling of RNA with ATP or cordycepin
    • Poly(A) tailing of RNA for cloning or affinity purification
    • Increasing translation of RNA transferred into eukaryotic cells


    Enzyme supplied with 5X Reaction Buffer.

    Concentration1 U/μl
    Storage Condition

    Store all components at -20°C.

    Storage Buffer20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.

    One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.

    This product is distributed for laboratory research only. Caution: Not for diagnostic use .


    Supporting Protocol



        There are no FAQs for this product yet!

        • Alghamdi, T.. "Insights into Glomerular Cell Biology in Health and Disease (Doctoral dissertation)" University of Toronto Toronto:Canada (2019).
        • Hou, W., Singh, R. K., Zhao, P., Martins, V., Aguilar, E., Canto, T., ... & Dias, A. C. P. "Transgenic expression of Hyp-1 gene from Hypericum perforatum L" alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens. Planta 251(1):13 (2020).