Poly(A) Polymerase, Yeast

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E017100 U (100 μl)
$45.00

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1 x PolyA Polymerase, Yeast   + $0.00
1 x 5X Reaction Buffer   + $0.00
1 x 25mM MnCl2   + $0.00
1 x ATP   + $0.00

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Poly(A) Polymerase, Yeast
Poly(A) Polymerase, Yeast

In stock

$45.00

Summary
    Specifications


    DescriptionPoly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
    SKUE017
    Unit quantity100 U (100 μl)
    Applications

    • Labelling of RNA with ATP or cordycepin • Poly(A) tailing of RNA for cloning or affinity purification • Increasing translation of RNA transferred into eukaryotic cells

    Components

    Enzyme supplied with 5X Reaction Buffer.

    Concentration1 U/μl
    Storage Condition

    Store all components at -20°C.

    Storage Buffer20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
    Note

    One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.

    This product is distributed for laboratory research only. Caution: Not for diagnostic use .

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          References


          2
          • Alghamdi, T.. "Insights into Glomerular Cell Biology in Health and Disease (Doctoral dissertation)" University of Toronto Toronto:Canada (2019).
          • Hou, W., Singh, R. K., Zhao, P., Martins, V., Aguilar, E., Canto, T., ... & Dias, A. C. P. "Transgenic expression of Hyp-1 gene from Hypericum perforatum L" alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens. Planta 251(1):13 (2020).