Poly(A) Polymerase, Yeast
|E017||100 U (100 μl)|
|Description||Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.|
|Unit quantity||100 U (100 μl)|
• Labelling of RNA with ATP or cordycepin • Poly(A) tailing of RNA for cloning or affinity purification • Increasing translation of RNA transferred into eukaryotic cells
Enzyme supplied with 5X Reaction Buffer.
Store all components at -20°C.
|Storage Buffer||20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.|
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
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