Pro Ligation-Free Cloning Kit (20 reactions)

Cat. No.
E086
Unit
20 Reactions
Price
$131.00
Cat. No. E086
Name Pro Ligation-Free Cloning Kit (20 reactions)
Unit 20 Reactions
Category Molecular Biology Enzymes and Kits
Description

The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs.

Key Kit Features:

  • One-step cloning: No more time-consuming, multi-step traditional cloning that depnds on available restrict sites or compatible ends
  • Scar-free cloning: No unwanted/extra base pairs in the inserts
  • Easy-to-customize: Clone in promoter, gene, tag, multi-fragment or whiole vector constructs in one step
  • Ready for downstream applications: Ligase-free reaction, no gel extraction or column purification steps needed
  • Robust assembly: Better performance for difficult, multi-fragment assemblies such as CRISPR multiplex sgRNA vector assembly
  • Increased thermostability: High colony yields

Testimonial:

"I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit."

Jianfeng Lan, The Buck Institute

Application
  • Rapid multi-fragment cloning and assembly of whole vector constructs
  • Clone in a promoter, gene, and tag, all in one step
  • Rapid assembly of CRISPR multiplex gRNA vectors in one step
Storage Condition

Store at -20°C

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E086
Search CoA here
  • Huang, R., Ding, R., Liu, Y., Li, F., Zhang, Z., & Wang, S. A. 2022. GATA transcription factor WC2 regulates the biosynthesis of astaxanthin in yeast Xanthophyllomyces dendrorhous. Microbial Biotechnology, 15(10), 2578-2593.
  • Hwang, B.-J., Gonzales, R., Corzine, S., Stenson, E., Pidugu, L., & Lu, A.-L. (2022). DNA binding by the Rad9A subunit of the Rad9-Rad1-Hus1 complex. PLOS ONE, 17(8), e0272645. https://doi.org/10.1371/journal.pone.0272645
  • Li, L., Liu, W., Fan, N., Li, F., Huang, B., Liu, Q., ... & Wang, X. (2022). Scallop IKK1 Responds to Bacterial and Virus-Related Pathogen Stimulation and Interacts With MyD88 Adaptor of Toll-Like Receptor Pathway Signaling. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.869845
  • Huang, B., Wu, Y., Ma, J., Yang, B., Sang, X., Chen, J., Liu, W., Li, F., Li, L., Wang, X., Dong, J., & Wang, X. (2022). The first identified invertebrate LGP2-like homolog gene in the Pacific oyster Crassostrea gigas. Fish & Shellfish Immunology, 128, 238–245. https://doi.org/10.1016/j.fsi.2022.08.005
  • Sang, X., Liu, W., Li, F., Huang, B., Li, L., Wang, X., Dong, J., Ma, J., Chen, J., & Wang, X. (2022). Scallop RIG-I-like receptor 1 responses to polyinosinic:polycytidylic acid challenge and its interactions with the mitochondrial antiviral signaling protein. Fish & Shellfish Immunology, 124, 490–496. https://doi.org/10.1016/j.fsi.2022.04.042
  • Li M., et al. 2022. A Chitin-Binding Protein Ultra-highly expressed in the Outer Fold of Mantle is Related to Shell Colour in Pacific Oyster Crassostrea gigas. https://doi.org/10.55277/researchhub.jg604pn4
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