Protein A Agarose Resin for antibody purification

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Specifications


Description

Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Protein A agarose beads are developed for purification of classes, subclasses and fragments of immunoglobulins, especially IgGs of different species. Protein A is a cell wall component isolated from Staphylococcus aureus that contains five antibody-binding domains with affinity for IgG, some IgA and IgM. This high affinity domain-Fc region of the immunoglobulins interaction makes one-step purification possible for proteins under native conditions. The immunoglobulins bind to Protein A, which are immobilized onto a solid support. Impurities are removed and the purified immunoglobulins can be eluted by decreasing the pH.

Special Features:
  • One-step easy purification protocol from crude lysate.
  • Purification under native conditions.
  • Can be used for successive cycles.
  • Ready-to-use for any scale of purification.
  • Protein binding capacity: approx. >25mg human IgG/ml gel.
  • Resins and columns formats suitable for batch and gravity purification.

SKU10011752
Applications
  • Purification of a wide range of immunoglobulins of different mammalian species.
  • Purification of certain IgG subclasses that have no affinity
  • Assays involving protein-protein interactions.
  • Structural and functional investigation of antibodies.
Documents


Supporting Protocol
MSDS

    QC

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