Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Protein A agarose beads are developed for purification of classes, subclasses and fragments of immunoglobulins, especially IgGs of different species. Protein A is a cell wall component isolated from Staphylococcus aureus that contains five antibody-binding domains with affinity for IgG, some IgA and IgM. This high affinity domain-Fc region of the immunoglobulins interaction makes one-step purification possible for proteins under native conditions. The immunoglobulins bind to Protein A, which are immobilized onto a solid support. Impurities are removed and the purified immunoglobulins can be eluted by decreasing the pH.
Special Features:
One-step easy purification protocol from crude lysate.
Purification under native conditions.
Can be used for successive cycles.
Ready-to-use for any scale of purification.
Protein binding capacity: approx. >25mg human IgG/ml gel.
Resins and columns formats suitable for batch and gravity purification.
SKU
10011752
Applications
Purification of a wide range of immunoglobulins of different mammalian species.
Purification of certain IgG subclasses that have no affinity
Assays involving protein-protein interactions.
Structural and functional investigation of antibodies.
Description
Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Protein A agarose beads are developed for purification of classes, subclasses and fragments of immunoglobulins, especially IgGs of different species. Protein A is a cell wall component isolated from Staphylococcus aureus that contains five antibody-binding domains with affinity for IgG, some IgA and IgM. This high affinity domain-Fc region of the immunoglobulins interaction makes one-step purification possible for proteins under native conditions. The immunoglobulins bind to Protein A, which are immobilized onto a solid support. Impurities are removed and the purified immunoglobulins can be eluted by decreasing the pH.
SKU
G243
Product Features
<div id="answer">
<ul>
<li>One-step easy purification protocol from crude lysate.</li>
<li>Purification under native conditions.</li>
<li>Can be used for successive cycles.</li>
<li>Ready-to-use for any scale of purification.</li>
<li>Protein binding capacity: approx. >25mg human IgG/ml gel.</li>
<li>Resins and columns formats suitable for batch and gravity purification.</li>
</ul>
</div>
Applications
Purification of a wide range of immunoglobulins of different mammalian species.
Purification of certain IgG subclasses that have no affinity
Assays involving protein-protein interactions.
Structural and functional investigation of antibodies.
Unit quantity
5.0 ml
Description
Affinity chromatography is a method of separating biochemical mixtures based on highly specific biological interactions. The Protein A agarose beads are developed for purification of classes, subclasses and fragments of immunoglobulins, especially IgGs of different species. Protein A is a cell wall component isolated from Staphylococcus aureus that contains five antibody-binding domains with affinity for IgG, some IgA and IgM. This high affinity domain-Fc region of the immunoglobulins interaction makes one-step purification possible for proteins under native conditions. The immunoglobulins bind to Protein A, which are immobilized onto a solid support. Impurities are removed and the purified immunoglobulins can be eluted by decreasing the pH.
SKU
G244
Product Features
<div id="answer">
<ul>
<li>One-step easy purification protocol from crude lysate.</li>
<li>Purification under native conditions.</li>
<li>Can be used for successive cycles.</li>
<li>Ready-to-use for any scale of purification.</li>
<li>Protein binding capacity: approx. >25mg human IgG/ml gel.</li>
<li>Resins and columns formats suitable for batch and gravity purification.</li>
</ul>
</div>
Applications
Purification of a wide range of immunoglobulins of different mammalian species.
Purification of certain IgG subclasses that have no affinity
Assays involving protein-protein interactions.
Structural and functional investigation of antibodies.
