qPCR Lentivirus Titer Kit
Cat. No.
LV900
Unit
100 rxn
Price
$195.00
Specifications
| Cat. No. | LV900 | |||||||||||||||||
| Name | qPCR Lentivirus Titer Kit | |||||||||||||||||
| Unit | 100 rxn | |||||||||||||||||
| Category | PCR, qPCR, and RT | |||||||||||||||||
| Description |
The only kit on the market with complete elimination of NTCabm’s qPCR Lentivirus Titer Kit is a one-step assay which employs a quick RNA extraction step that is followed by RT-qPCR. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments. Kit Features:
|
|||||||||||||||||
| Storage Condition |
Store at -20°C. This product is stable for 2 year from the date of shipping if stored and handled properly. |
|||||||||||||||||
| Note |
qPCR Lentivirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
|
Documents
Supporting Protocol
MSDS
FAQs
| What is the primer design based on? | |
|
The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
|
| How come my cells are not successfully infected with the evaluated titer from the kit? | |
|
One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.
|
References
- Bushnell, G. G., Rao, S. S., Hartfield, R. M., Zhang, Y., Oakes, R. S., Jeruss, J. S., & Shea, L. D. "Microporous scaffolds loaded with immunomodulatory lentivirus to study the contribution of immune cell populations to tumor cell recruitment in vivo" Biotechnology and bioengineering. : (2019).
- Johnson, J. O., Chia, R., Brown Jr, R. H., & Landers, J. E. "Mutations in the SPTLC1 gene are a cause of amyotrophic lateral sclerosis that may be amenable to serine supplementation" : (2019).
- Keshwara, R., Hagen, K. R., Abreu-Mota, T., Papaneri, A. B., Liu, D., Wirblich, C., … Schnell, M. J. "A Recombinant Rabies Virus Expressing the Marburg Virus Glycoprotein Is Dependent upon Antibody-Mediated Cellular Cytotoxicity for Protection against Marburg Virus Disease in a Murine Model" Journal of Virology 93(6): (2018). DOI: 10.1128/jvi.01865-18.
- Long, K. L. P.. "The role of oligodendrocytes and myelin in differential susceptibility to stress-induced anxiety (Doctoral Thesis)" University of California Berkeley:United States (2018).
- Rahal, O. M., Nie, L., Chan, L. C., Li, C. W., Hsu, Y. H., Hsu, J., ... & Hung, M. C. "Selective expression of constitutively active pro-apoptotic protein BikDD gene in primary mammary tumors inhibits tumor growth and reduces tumor initiating cells" American journal of cancer research 5(12):3624 (2015).
- Schatoff, E. M., Zafra, M. P., & Dow, L. E. "Base editing the mammalian genome" Methods 164–165:100–108 (2019). DOI: 10.1016/j.ymeth.2019.02.022.
- Scheben, A., Wolter, F., Batley, J., Puchta, H., & Edwards, D. "Towards CRISPR/Cas crops - bringing together genomics and genome editing" New Phytologist 216(3):682–698 (2017). DOI: 10.1111/nph.14702.
- Tsai, . "U" S. Patent Application No.0015473 A1 : (2019).
Controls and Related Product: