RecBCD (Exonuclease V)
|E093||100 U (100 μl)|
|Description||RecBCD (Exonuclease V) is a Mg2 and ATP-dependent enzyme possessing exonuclease activity on linear double stranded (ds) DNA. The exonuclease digests DNA from both the 5’ and 3’ ends to produce oligonucleotides. RecBCD (Exonuclease V) does not degrade circular dsDNA such as plasmid DNA.|
|Unit quantity||100 U (100 μl)|
|Format||RecBCD enzyme supplied with 10X RecBCD Reaction Buffer. ATP is not provided. Incubate at 37°C.|
|Unit Definition||One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in 30 minutes at 37°C in a total reaction volume of 50 µl.|
|Storage Buffer||50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.5 @ 25°C.|
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
There are no FAQs for this product yet!
There are no references for this product yet!