Recombinant Human 4-1BB Receptor (TNFRSF9) (E. coli)
|Description||4-1BB Receptor (CD137) is a member of the TNF receptor superfamily expressed on activated CD4 and CD8 T cells as well as on activated natural killer cells. The binding of 4-1BB Receptor to its TNF family ligand, 4-1BB ligand (4-1BBL) delivers a co-stimulatory signal to resting T cells leading to high level IL2 production independently of CD28 signaling. Recombinant Human 4-1BB Receptor represents the soluble, cysteine-rich TNFR-like extracellular domain of 4-1BB Receptor.|
|Growth Factor Size||1.0 mg|
|Unit quantity||1.0 mg|
|Family||Other Growth Factors/Cytokines|
|Aliases||Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, CD137|
Recombinant Human 4-1BB (TNFRSF9)
|Species||Human (H. sapiens)|
|Molecular Weight||18 kDa|
|Endotoxin Level||<1.0 EU/µg of recombinant protein as determined by the LAL method.|
|Purity||>95% as determined by SDS-PAGE|
|Formulation||Lyophilized from 0.2 μm filtered solution in PBS (pH 7.5)|
|Function||The ED50 as determined by dose dependent proliferation of human megakaryocytic leukemia cell line is <0.5ng/ml.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||Recombinant 4-1BB Receptor can be stored in working aliquots at 2° - 8°C for one month, or at -20°C to -70°C for twelve months. Avoid repeated freeze/thaw cycles.|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
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|How are endotoxin levels measured?|
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970. · Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL). · For recombinant proteins: EU is reported per microgram of protein. · One EU = 0.1-0.2 ng endotoxin/µg of protein. · At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
|Which residues does the protein sequence span?|
The construct spans residue 19 – 184 (predicted extracellular domain).
|With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?|
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
|Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?|
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.
|Is this untagged or tagged?|
This protein is untagged.
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