Recombinant Human IL10
Cat. No.
Z101195
Unit
25 µg
Price
$660.00
| Cat. No. | Z101195 |
| Name | Recombinant Human IL10 |
| Description | IL-10 is produced by mouse Th2 cells following their stimulation by lectins. The main source for B cell derived IL-10 in mice are Ly1 B cells that express CD5 (Ly1) and CD11. In humans IL-10 is produced by activated CD8(+) peripheral blood T cells, by T helper CD4(+) T cell clones after both antigen-specific and polyclonal activation, by B cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL-10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin-10 shows 73% amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL-10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL-10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL-2 and TNF-beta in Th1 T helper subpopulations of T cells but not of Th2 T helper cells. This activity is antagonized by IL-4. The inhibitory effect on IFN-gamma production is indirect and appears to be the result of a suppression of IL-12 synthesis by accessory cells. In the human system, IL-10 is produced by, and down-regulates the function of Th1 and Th2 cells. |
| Organism | Human (H. sapiens) |
| Unit quantity | 25 µg |
| Family | IL-10/Interferon Family |
| Aliases |
IL-10, Cytokine synthesis inhibitory factor, CSIF, B-TCGF, TGIF Recombinant Human Interleukin-10 (IL10) |
| Gene Symbol | IL10 |
| Gene ID | 3586 |
| Accession Number | P22301 |
| Source | CHO cells |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 19 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Recombinant Interleukin-10 was lyophilized from a 0.2 μm filtered PBS solution pH 7.0. |
| Function | The ED(50) determined by dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED(50) was found to be ≤ 2.0 ng/mL, corresponding to a specific activity of ≥ 4.0 x 10^5 units/mg. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101195 |
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