Recombinant Mouse IL3

Cat. No.
Z200145
Unit
Price
$372.00
Cat. No. Z200145
Name Recombinant Mouse IL3
Description IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial-derived recombinant IL3 shows that glycosylation is not required for the activity of IL3. IL-3 sequences are evolutionarily less well conserved. Human and murine IL3 show approximately 29% homology at the protein level while murine and rat IL3 show approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant mouse IL3 is a monomer of 15 kDa.
Organism Mouse (M. musculus)
Unit quantity 10 µg
Family Other Interleukins
Aliases IL-3, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, Multi-CSF, HCGF, P-cell stimulation factor, Hematopoietic growth factor
Recombinant Mouse Interleukin-3 (IL3)
Gene Symbol IL3
Gene ID 16187
Accession Number P01586
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 15 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Function The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells was found to be in the <0.05 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200145
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