RFP Stably Expressing MCF7 Cell Line
|T3932||1x106 cells / 1.0 ml|
|Species||Human (H. sapiens)|
|Population Doubling Time||24-36 hours|
|Unit quantity||1x106 cells / 1.0 ml|
|Propagation Requirements||The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium with the final concentration: fetal bovine serum (TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.|
|QC||Puromycin drug selection|
|Caution||For Research Use Only|
|Cell Type||Reporter Cells|
|Shipping Conditions||Dry Ice|
|Is the GFP or RFP signal localized within the cell?|
|What type of GFP or RFP do these cell lines express, and from which promoter?|
The GFP is derived from copGFP (from Pontellina plumata) and the RFP is derived from mKate2 (from Entacmaea quadricolor). They are expressed from a proximal SV40 promoter, and a more distal CMV promoter may also drive expression. If you are looking for a cell line with a specific fluorescent reporter, or with a specific promoter, abm can perform a Stable Cell Line Generation service. Simply ask [email protected] for a quote.
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