RNase H, E. coli
|E018||500 U (100 μl)|
|Description||RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase H does not digest single-stranded or double-stranded DNA and RNA.|
|Unit quantity||500 U (100 μl)|
|Format||Enzyme supplied with 10X Reaction Buffer|
Store all components at -20°C.
|Storage Buffer||20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol.|
One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
There are no references for this product yet!