RNaseOFF Ribonuclease Inhibitor
|G138||4,000 U (100 µl)|
|Description||RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseOFF Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.|
|Unit quantity||4,000 U (100 µl)|
|Storage Condition||Store all components at -20°C.|
|Note||One unit is defined as the amount of RNaseOFF Ribonuclease Inhibitor that is required to inhibit the activity of 5 ng of RNase A by 50%.|
• Always keep RNaseOFF on ice during reaction set-up.
• It is recommended to setup all RNA-related/RNase-sensitive work under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% DEPC.
• Wearing gloves is highly recommended while working with RNA.
This product is distributed for laboratory research only Caution: Not for diagnostic use.
|Are all components in this product RNase-free?|
Yes, all components in the RNaseOFF Ribonuclease Inhibitor is RNase-free.
|What is the shelf life of this reagent?|
1 year upon shipment of the reagent.
|How many reactions can be performed?|
The appropriate number of units per reaction will vary depending on the amount of RNase contamination that is present in the reaction mixture for any given experiment. One unit of RNaseOFF Ribonuclease Inhibitor will inhibit the activity of 5ng of RNase by 50%; thus, the more RNase contamination present in the specific reaction, the larger number of units of RNaseOFF that will be required for the inhibition. As a general guideline, a typical amount of RNaseOFF used would be around 25-50U per 50ul reaction; note that this is a general guideline only and the number of units required will vary from case to case.
- Del Toro, N., Lessard, F., Tardif, S., Bouchard, J., Bourdeau, V., Ferbeyre, G., & Brakier-Gingras, L. "The retinoblastoma tumor suppressor limits ribosomal readthrough during oncogene induced senescence" bioRxiv 788380: (2019).