Safe-Green™ 100bp Opti-DNA Marker
|G473||500 μl/100 loads|
Safe-Green™ Opti-DNA Markers contain Safe-Green™ DNA stain which is a non-toxic and efficient alternative DNA visualizing agent to the carcinogenic ethidium bromide (EB). The brightness of Safe-Green™ Opti-DNA Markers is optimized for compatible and proper camera exposure in applications along with PCR products generated from abm’s Safe-Green™ 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification.
To use Safe-Green™ Opti-DNA Markers, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV or LED blue light source. Safe-Green™ has an excitation maximum at 495 nm and an emission maximum at 520 nm.
For approximating DNA size and amount on agarose gels.
|Suggested Load||Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.|
Shipped on blue ice packs.
Store @ 4°C for up to 3 months or -20°C for up to 24 months.
|Unit quantity||500 μl/100 loads|
|Is it ready for use?|
Yes, it is ready to use. Just load suggested volume (5ul) to the well.
|What is the smallest size agarose gel can separate?|
That is around 300bp for 1% agarose and 100bp for 2.5% agarose. With anything smaller than the dye, there may be separation but it will be difficult to tell when the fragment will run off the gel.
|Are the Opti-DNA markers compatible with radiolabelling?|
Our lab has not tested the Opti-DNA markers for this particular application, but we believe it shouldn't be a problem. The Opti-DNA markers should be compatible for radio-labelling.
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