Safe-Green™ 1kb Opti-DNA Marker

Cat. No.
G474
Unit
500 μl/100 loads
Price
$73.50
Cat. No. G474
Name Safe-Green™ 1kb Opti-DNA Marker
Unit 500 μl/100 loads
Category Gels, Blots & ELISAs
Description

Safe-Green™ Opti-DNA Markers contain Safe-Green™ DNA stain which is a non-toxic and efficient alternative DNA visualizing agent to the carcinogenic ethidium bromide (EB). The brightness of Safe-Green™ Opti-DNA Markers is optimized for compatible and proper camera exposure in applications along with PCR products generated from abm’s Safe-Green™ 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification. To use Safe-Green™ Opti-DNA Markers, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source. Safe-Green™ has an excitation maximum at 495 nm and an emission maximum at 520 nm.

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 19 bands, ranging from 100 pb – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. Total DNA concentration for all bands combined is 103.2ng/μl.

Number of Bands          19

DNA Range                     100bp-10kb

Suggested Load              Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.

Application

For approximating DNA size and amount on agarose gels.

Storage Condition

Store @ 4°C for up to 3 months or -20°C for up to 24 months.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G474
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