Safe-Green™ 1kb Opti-DNA Marker
CAT.NO | UNIT | PRICE |
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G474 | 500 μl/100 loads | $70.00 |
Description | Safe-Green™ Opti-DNA Markers contain Safe-Green™ DNA stain which is a non-toxic and efficient alternative DNA visualizing agent to the carcinogenic ethidium bromide (EB). The brightness of Safe-Green™ Opti-DNA Markers is optimized for compatible and proper camera exposure in applications along with PCR products generated from abm’s Safe-Green™ 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification. To use Safe-Green™ Opti-DNA Markers, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source. Safe-Green™ has an excitation maximum at 495 nm and an emission maximum at 520 nm. |
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SKU | G474 |
Applications | For approximating DNA size and amount on agarose gels. |
Bands | 19 |
DNA Range | 100bp-10kb |
Suggested Load | Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel. |
Shipping Conditions | Shipped on blue ice packs. |
Storage Condition | Store @ 4°C for up to 3 months or -20°C for up to 24 months. |
Unit quantity | 500 μl/100 loads |
Is it ready for use? | |
Yes, it is ready to use. Just load suggested volume (5ul) to the well. |
What is the smallest size agarose gel can separate? | |
That is around 300bp for 1% agarose and 100bp for 2.5% agarose. With anything smaller than the dye, there may be separation but it will be difficult to tell when the fragment will run off the gel.
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Are the Opti-DNA markers compatible with radiolabelling? | |
Our lab has not tested the Opti-DNA markers for this particular application, but we believe it shouldn't be a problem. The Opti-DNA markers should be compatible for radio-labelling. |
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