SARS-CoV-2 RNA-dependent RNA Polymerase (RDRP) AAV Virus
|COV00806||5 x 200 μl|
|Gene Name||SARS-CoV-2 RNA-dependent RNA Polymerase (RDRP)|
atgtcagctg atgcacaatc gtttttaaac cgggtttgcg gtgtaagtgc agcccgtctt acaccgtgcg gcacaggcac tagtactgat gtcgtataca
|Unit quantity||5 x 200 μl|
|Serotype||AAV Serotype 6|
AAV viruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Store AAV viruses at -80°C. Avoid repeated freeze-thaw cycles by storing in small aliquots.
We will provide qPCR based titer evaluation for standard AAV virus orders as determination of successful packaging. In addition, if your virus has a GFP or RFP reporter (with no induction required for expression), we will also perform viral infectivity testing in our standard evaluation cell line - HEK293T. An image of the infectivity results will be provided on the CoA. Since infectivity is serotype dependent, and HEK293T cells do not infect well with Serotypes 4,5 and 6, an infectivity test will not be provided for these serotypes. In such cases, qPCR titer evaluation will be used as the final parameter to determine successful viral packaging.
abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data or a western blot to evaluate the level of gene expression. The replacement will not be covered by the same guarantee. Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.
- Wang, Q., Yu, H., Yu, H., Ma, M., Ma, Y., & Li, R. "miR‑223‑3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in�vitro" Molecular Medicine Reports. : (2018). DOI: 10.3892/mmr.2018.9742.
- Xu, T., He, B. S., Pan, B., Pan, Y. Q., Sun, H. L., Liu, X. X., ... & Wang, S. K. "MiR‐142‐3p functions as a tumor suppressor by targeting RAC1/PAK1 pathway in breast cancer" Journal of Cellular Physiology. : (2019).
- Zhang, B., Roosmalen, I. A. M., Reis, C. R., Setroikromo, R., & Quax, W. J. "Death receptor 5 is activated by fucosylation in colon cancer cells" The FEBS Journal 286(3):555–571 (2019). DOI: 10.1111/febs.14742.
- Zhao, Q., Zhao, S., Li, J., Zhang, H., Qian, C., Wang, H., … Zhao, Y. "TCF7L2 activated HOXA-AS2 decreased the glucocorticoid sensitivity in acute lymphoblastic leukemia through regulating HOXA3/EGFR/Ras/Raf/MEK/ERK pathway" Biomedicine & Pharmacotherapy 109:1640–1649 (2019). DOI: 10.1016/j.biopha.2018.10.046.