SARS-CoV-2 RNA-dependent RNA Polymerase (RDRP) AAV Virus

CAT.NOUNITPRICE
COV008065 x 200 μl
$795.00

Specifications


SKUCOV00806
Gene NameSARS-CoV-2 RNA-dependent RNA Polymerase (RDRP)
Insert Size

2799bp

atgtcagctg atgcacaatc gtttttaaac cgggtttgcg gtgtaagtgc agcccgtctt acaccgtgcg gcacaggcac tagtactgat gtcgtataca
gggcttttga catctacaat gataaagtag ctggttttgc taaattccta aaaactaatt gttgtcgctt ccaagaaaag gacgaagatg acaatttaat
tgattcttac tttgtagtta agagacacac tttctctaac taccaacatg aagaaacaat ttataattta cttaaggatt gtccagctgt tgctaaacat
gacttcttta agtttagaat agacggtgac atggtaccac atatatcacg tcaacgtctt actaaataca caatggcaga cctcgtctat gctttaaggc
attttgatga aggtaattgt gacacattaa aagaaatact tgtcacatac aattgttgtg atgatgatta tttcaataaa aaggactggt atgattttgt
agaaaaccca gatatattac gcgtatacgc caacttaggt gaacgtgtac gccaagcttt gttaaaaaca gtacaattct gtgatgccat gcgaaatgct
ggtattgttg gtgtactgac attagataat caagatctca atggtaactg gtatgatttc ggtgatttca tacaaaccac gccaggtagt ggagttcctg
ttgtagattc ttattattca ttgttaatgc ctatattaac cttgaccagg gctttaactg cagagtcaca tgttgacact gacttaacaa agccttacat
taagtgggat ttgttaaaat atgacttcac ggaagagagg ttaaaactct ttgaccgtta ttttaaatat tgggatcaga cataccaccc aaattgtgtt
aactgtttgg atgacagatg cattctgcat tgtgcaaact ttaatgtttt attctctaca gtgttcccac ctacaagttt tggaccacta gtgagaaaaa
tatttgttga tggtgttcca tttgtagttt caactggata ccacttcaga gagctaggtg ttgtacataa tcaggatgta aacttacata gctctagact
tagttttaag gaattacttg tgtatgctgc tgaccctgct atgcacgctg cttctggtaa tctattacta gataaacgca ctacgtgctt ttcagtagct
gcacttacta acaatgttgc ttttcaaact gtcaaacccg gtaattttaa caaagacttc tatgactttg ctgtgtctaa gggtttcttt aaggaaggaa
gttctgttga attaaaacac ttcttctttg ctcaggatgg taatgctgct atcagcgatt atgactacta tcgttataat ctaccaacaa tgtgtgatat
cagacaacta ctatttgtag ttgaagttgt tgataagtac tttgattgtt acgatggtgg ctgtattaat gctaaccaag tcatcgtcaa caacctagac
aaatcagctg gttttccatt taataaatgg ggtaaggcta gactttatta tgattcaatg agttatgagg atcaagatgc acttttcgca tatacaaaac
gtaatgtcat ccctactata actcaaatga atcttaagta tgccattagt gcaaagaata gagctcgcac cgtagctggt gtctctatct gtagtactat
gaccaataga cagtttcatc aaaaattatt gaaatcaata gccgccacta gaggagctac tgtagtaatt ggaacaagca aattctatgg tggttggcac
aacatgttaa aaactgttta tagtgatgta gaaaaccctc accttatggg ttgggattat cctaaatgtg atagagccat gcctaacatg cttagaatta
tggcctcact tgttcttgct cgcaaacata caacgtgttg tagcttgtca caccgtttct atagattagc taatgagtgt gctcaagtat tgagtgaaat
ggtcatgtgt ggcggttcac tatatgttaa accaggtgga acctcatcag gagatgccac aactgcttat gctaatagtg tttttaacat ttgtcaagct
gtcacggcca atgttaatgc acttttatct actgatggta acaaaattgc cgataagtat gtccgcaatt tacaacacag actttatgag tgtctctata
gaaatagaga tgttgacaca gactttgtga atgagtttta cgcatatttg cgtaaacatt tctcaatgat gatactctct gacgatgctg ttgtgtgttt
caatagcact tatgcatctc aaggtctagt ggctagcata aagaacttta agtcagttct ttattatcaa aacaatgttt ttatgtctga agcaaaatgt
tggactgaga ctgaccttac taaaggacct catgaatttt gctctcaaca tacaatgcta gttaaacagg gtgatgatta tgtgtacctt ccttacccag
atccatcaag aatcctaggg gccggctgtt ttgtagatga tatcgtaaaa acagatggta cacttatgat tgaacggttc gtgtctttag ctatagatgc
ttacccactt actaaacatc ctaatcagga gtatgctgat gtctttcatt tgtacttaca atacataaga aagctacatg atgagttaac aggacacatg
ttagacatgt attctgttat gcttactaat gataacactt caaggtattg ggaacctgag ttttatgagg ctatgtacac accgcataca gtcttacag

Unit quantity5 x 200 μl
Titer>109 GC/ml
Accession NumberYP_009725307.1
FormatVirus
SystemAAV Virus
SerotypeAAV Serotype 6
SpeciesSARS-CoV-2
Vector Map

pAAV-G-CMV-amp

Shipping Conditions

AAV viruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.

Storage Condition

Store AAV viruses at -80°C. Avoid repeated freeze-thaw cycles by storing in small aliquots.

Disclaimer

We will provide qPCR based titer evaluation for standard AAV virus orders as determination of successful packaging. In addition, if your virus has a GFP or RFP reporter (with no induction required for expression), we will also perform viral infectivity testing in our standard evaluation cell line - HEK293T. An image of the infectivity results will be provided on the CoA. Since infectivity is serotype dependent, and HEK293T cells do not infect well with Serotypes 4,5 and 6, an infectivity test will not be provided for these serotypes. In such cases, qPCR titer evaluation will be used as the final parameter to determine successful viral packaging.

Guarantee

abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data or a western blot to evaluate the level of gene expression. The replacement will not be covered by the same guarantee. Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.

Documents


Supporting Protocol

    MSDS

      QC

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        References


        4
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        • Xu, T., He, B. S., Pan, B., Pan, Y. Q., Sun, H. L., Liu, X. X., ... & Wang, S. K. "MiR‐142‐3p functions as a tumor suppressor by targeting RAC1/PAK1 pathway in breast cancer" Journal of Cellular Physiology. : (2019).
        • Zhang, B., Roosmalen, I. A. M., Reis, C. R., Setroikromo, R., & Quax, W. J. "Death receptor 5 is activated by fucosylation in colon cancer cells" The FEBS Journal 286(3):555–571 (2019). DOI: 10.1111/febs.14742.
        • Zhao, Q., Zhao, S., Li, J., Zhang, H., Qian, C., Wang, H., … Zhao, Y. "TCF7L2 activated HOXA-AS2 decreased the glucocorticoid sensitivity in acute lymphoblastic leukemia through regulating HOXA3/EGFR/Ras/Raf/MEK/ERK pathway" Biomedicine & Pharmacotherapy 109:1640–1649 (2019). DOI: 10.1016/j.biopha.2018.10.046.