Show Results for:"c208"
You may want filter by Cat.No, Gene Name
- Cat. No.: C208
- Price: Inquiry
- Unit:Basic Service Package
Our Basic Package (C208) Includes Deliverables
- Cell line Validation (e.g. infection condition optimization, drug selection evaluation, Single cell clonability evaluation etc.)
- sgRNA vector design, construction & delivery into cells
- Clonal screening and selection
- Screening & validation of knockout by Sanger sequencing**
- Expansion & cryopreservation of selected clone(s)
- Microbial/sterility testing
Sequence verified knockouts (2 clones, 1 vial per clone)**, microbial/sterility tested with a service report. Add-On Services^ Quantity Cat. No. Price WT Control Cell Line Expressing Cas9 for Comparison
(50% discount for academic customers if ordered with the basic package)
1 Cell Line C142 $1,590.00 Additional Clones 1 Clone C143 $350.00 Additional Vials of Delivered Cells 1 Vial C144 $300.00 Western Blot Validation Service (Up to 10 Clones Tested)## 1 Service C145 $975.00 Cell Line Authentication:
STR Profiling of 1 WT Human Parental Cell Line & 1 Human Knock-Out Cell Line Clone
(For other species, please inquire)
1 Service C287 $195.00
*A deposit is required to initiate all CRISPR Cell Line Projects
**Rate is valid for academics only. For for-profit organizations and corporations, please inquire
**Choose from HEK293, HEK293T, A549, HeLa, MDCK, A375, HepG2, HT1080 or U87MG. These cell lines are available as Cas-9 expressing versions or parental versions. Cas9 expressing version will be used by default. If the parental version is preferred, please request prior to order placement.
**The above pricing is valid for a single gene knockout for diploid loci only.
#Customer is required to provide at least 2 million cells, 1L of propagation media/any special coated flasks if needed (if cells need DMEM or RPMI, abm will supply media). Also, the cell line must tolerate single cell cloning and display adequate transfection and transduction efficiency.
##Pre-validated antibody needs to be provided by customer with appropriate positive control (preferably with supporting data).
^All add on services need to be indicated prior to order placement as the project design will need to be finalized prior to start of the service.
^^ Final Lead time may vary depending on actual growth rate of cell line while expanding from single cell.
Please note that if the gene to be knocked-out may be essential to cell survival, it is up to the end-user to proceed with the services. abm is unable to guarantee cell survival in these cases and will only attempt to rescue the clones under these conditions. abm is not accountable for cell survival if rescuing the clones (instructions to be provided customers) is unsuccessful.
^^^ Additional media or supplements may be required. For for-profit organizations and corporations, the price is 1.5 times the listing price.