Serum-Free ExPAND T Cell Culture Complete Medium (With Cytokines & T Cell Activators)
abm has developed a specially formulated Serum-Free ExPAND T Cell culture medium for in vitro culture and optimal expansion of human T cells. It has already been reported that T cells expanded in the absence of human serum provide durable control of tumors. Engineered T cells expanded in an optimized medium in the absence of serum may improve therapeutic potential.
Our Serum-Free ExPAND T Cell Culture Complete Medium is a defined xeno-free formulation, and does not contain bovine or other non-human, animal-derived components. It exhibits greater T cell expansion when compared to one of the most popular T cell expansion media currently available in the market, and also a T cell medium with serum. Additionally, Flow cytometry and Next generation sequencing (NGS) of primary human T cells cultured in abm’s Serum-Free ExPAND T Cell Culture Medium showed that it maintains the expression profile of crucial T cell markers. Furthermore, it supports efficient lentiviral transduction of primary human T cells, allowing it be used as an ideal medium for CAR-T applications.
This kit has following components:
To make complete supplemented media, add T Cell Expansion Supplement to 470 ml basal medium, and add required amounts of T cell Cytokine Supplement and T cell Activation Supplement according to your application (Please refer to the data sheet for details). This complete medium need not to be supplemented with cytokines or T cell activators as a proprietary combination of IL-2 and CD3/CD28 T cell activators has already been included in the provided supplements.
The optimal storage temperature of Basal Medium and T Cell Activation Supplement is 2-8°C. Protect from light. Do not freeze. The expiry date is indicated on the label.
Each lot has been fully tested and optimized for the ability to support the in vitro culture of human T cells. The parameters of testing include testing cell proliferation, viability and typical morphology of T cells.
For research use only and is not intended for therapeutic or diagnostic applications.
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