Serum-Free ExPAND T Cell Culture Medium
abm has developed a specially formulated Serum-Free ExPAND T Cell culture medium for in vitro culture and optimal expansion of human T cells. It has already been reported that T cells expanded in the absence of human serum provide durable control of tumors. Engineered T cells expanded in an optimized medium in the absence of serum may improve therapeutic potential.
Our Serum-Free ExPAND T Cell Culture Medium is a defined xeno-free formulation, and does not contain bovine or other non-human, animal-derived components. It exhibits greater T cell expansion when compared to one of the most popular T cell expansion media currently available in the market and also a T cell medium with serum. Additionally, Flow cytometry and Next generation sequencing (NGS) of primary human T cells cultured in abm’s Serum-Free ExPAND T Cell Culture Medium showed that it maintains the expression profile of crucial T cell markers. Furthermore, it supports efficient lentiviral transduction of primary human T cells, allowing it be used as an ideal medium for CAR-T applications.
This kit has following components:
To make supplemented medium, add 30 ml T Cell Expansion Supplement to 470 ml of basal medium. This supplemented medium must be appended with cytokines such as recombinant human IL-2 (Cat. #Z100489) or other cytokines as per desired application. Stimulation with T cell activation reagents like soluble or plate-bound CD3 antibody or CD3/CD28 antibodies is also required, but not provided.
The optimal storage temperature of Basal Medium is 2-8°C. Protect from light. Do not freeze. The expiry date of the medium is listed on the bottle. T Cell Expansion Supplement can be stored at -20 °C.
Each lot has been fully tested and optimized for the ability to support the in vitro culture of human T cells. The parameters of testing include testing cell proliferation, viability and typical morphology of T cells.
For research use only and is not intended for therapeutic or diagnostic applications.
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