SmaI

CAT.NOUNITPRICE
E07410000 U (500 μl)
$360.00

Specifications


SKUE074
Unit quantity10000 U (500 μl)
Applications

Restriction enzyme digestion of double-stranded DNA.

Expression SystemAn E. coli strain that carries the cloned and modified SmaI gene from Serratia marcescens (ATCC 49779). The SmaI enzyme recognizes and cuts the sequence CCC^GGG.
Inactivation Protocol65°C for 20 min
Reaction Definition1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C.
Reaction Buffer

Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%
Click here to view the buffer compatibility chart for restriction enzymes.

Storage Condition

-20°C

Storage Buffer50 % Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, 0.1 mM EDTA, pH 7.4 @ 25°C.
Note

Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Documents


Supporting Protocol
MSDS

    QC
    Other

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