SpeI

CAT.NOUNITPRICE
E0752500 U (250 μl)
$390.00

Specifications


SKUE075
Unit quantity2500 U (250 μl)
Applications

Restriction enzyme digestion of double-stranded DNA.

Components

SpeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204).

Concentration10000 U/ml
Expression SystemAn E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). The SpeI enzyme recognizes and cuts the sequence A^CTAGT.
Inactivation Protocol80°C for 20 min
Reaction Definition1X Universal Restriction Enzyme Buffer (Cat.No. E204). Incubate at 37°C.
Reaction Buffer

Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%
Click here to view the buffer compatibility chart for restriction enzymes.

Storage Condition

-20°C

Storage Buffer0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 200 µg/ml BSA, 250 mM NaCl, 0.15% Triton®X-100, pH 7.4 @ 25°C.
Note

Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Documents


Supporting Protocol
MSDS

    QC
    Other

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