Stable Mgat2 Knockout K16 CHO Cell Line

T60081x106 cells / 1.0 ml


DescriptionUsing CRISPR technology Mgat2 was silenced from the K16 CHO cells to generate a glycosylation mutant cell line which expresses hybrid type N-glycans. Mgat2 synthesizes the Mannosyl (Alpha-1,6-)-Glycoprotein Beta-1,2-N-Acetylglucosaminyltransferase enzyme (GlcNAcT-II) which plays a key role in the development of mammals in which carbohydrate-dependent interactions facilitate cell signaling, adhesion and migration. Carbohydrate-carbohydrate interaction by the hybrid type N-glycans has been shown to induce faster cell-cell adhesion and cell migration. Further research and discovery on the functions of N-glycans can be assessed with the Stable Mgat2 Knockout K16 CHO Cell Line.
SpeciesGolden Hamster (M. auratus)
Species descriptionCricetulus griseus (Chinese hamster)
Tissue/Organ/Organ SystemReproductive
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

For Research Use Only

Mammalian Selection MarkerPuromycin
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetEnzymes
CautionFor Research Use Only
Gene Knockdown MethodMgat2 (UDP-N-acetylglucosamine:-6-D-mannoside 1,2-N-acetylglucosaminyltransferase II) silenced through C residue inserted after the 22nd nucleotide residue causing frameshift mutation
Cell TypeDrug Discovery Cells
Expression Profile


Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow VIII medium available at abm (TM008). To make the complete growth medium, add the following components to the base medium to final concentration of fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

Selection: 4 μg/ml puromycin (G264.)

1) 4 µg/ml of puromycin (G264) was used for clonal selection; 2) Mgat2 silencing was confirmed by DNA sequencing of targeted genomic region; 3) E-cadherin was determined through the use of Western blot method


1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorEastern Carolina University

Supporting Protocol




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      • Hall, MK et al. "Predominant Expression of Hybrid N-Glycans Has Distinct Cellular Roles Relative to Complex and Oligomannose N-Glycans" Int J Mol Sci. 17(6):E925 (2016). DOI: 10.3390/ijms17060925.