T4 DNA Ligase
|G467||200 U (200 μl)|
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
|Unit quantity||200 U (200 μl)|
• Cloning of restriction fragments
|Format||Enzyme supplied with 5X Reaction Buffer|
Store all components at -20°C.
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
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