T4 DNA Ligase

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G467200 U (200 μl)
$145.00

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T4 DNA Ligase
T4 DNA Ligase

In stock

$145.00

Summary
    Specifications


    Description

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.

    SKUG467
    Unit quantity200 U (200 μl)
    Applications

    • Cloning of restriction fragments
    • Attaching double-stranded oligonucleotide linkers or adaptors to DNA
    • Site-directed mutagenesis
    • Nick repair of duplex DNA, RNA or DNA-RNA hybrids
    • Self-circularization of linear DNA

    Concentration1 U/μl
    FormatEnzyme supplied with 5X Reaction Buffer
    Storage Condition

    Store all components at -20°C.

    Note

    This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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