T4 DNA Ligase

Cat. No.
G467
Unit
80,000 U (200 μl)
Price
$152.00
Cat. No. G467
Name T4 DNA Ligase
Unit 80,000 U (200 μl)
Category Molecular Biology Enzymes and Kits
Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.

Application

• Cloning of restriction fragments
• Attaching double-stranded oligonucleotide linkers or adaptors to DNA
• Site-directed mutagenesis
• Nick repair of duplex DNA, RNA or DNA-RNA hybrids
• Self-circularization of linear DNA

Concentration 400 U/μl
Format General Enzyme supplied with 5X Reaction Buffer
Reaction Definition One unit is the enzyme quantity needed to achieve 50% ligation of HindIII fragments of λ DNA (with 5´ DNA termini concentration of 0.12 µM, and 300 µg/ml) in a 20 μl total reaction volume, within 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer.
Storage Condition

Store all components at -20°C.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G467
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