T4 DNA Polymerase
|E005||500 U (100 μl)|
|Description||T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.|
|Unit quantity||500 U (100 μl)|
|Format||Enzyme supplied with 10X Reaction Buffer|
Store all components at -20°C.
|Storage Buffer||100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.|
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
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