T4 DNA Polymerase

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E005500 U (100 μl)
$95.00

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T4 DNA Polymerase
T4 DNA Polymerase

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$95.00

Summary
    Specifications


    DescriptionT4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
    SKUE005
    Unit quantity500 U (100 μl)
    Applications
    • Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs
    • High fidelity due to strong 3’→5’ exonuclease activity
    • Synthesis of labeled DNA probes by the replacement reaction
    • Site-specific mutagenesis via primer extension from oligonucleotides
    Concentration5 U/μl
    FormatEnzyme supplied with 10X Reaction Buffer
    Storage Condition

    Store all components at -20°C.

    Storage Buffer100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.
    Note

    One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
    This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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