U6-2 Primers

Cat. No.
MPH00001
Unit
150 μl / 10 μM
Price
$73.50

Lead Time
3 to 4 weeks
Cat. No. MPH00001
Name U6-2 Primers
Unit 150 μl / 10 μM
Description

abm miRNA primers are for qPCR applications and each miRNA-specific primer set comes with a universal reverse primer. All miRNA primers are supplied in 10μM format.

miRNA Name U6-2
Accession Number NR_002752
Species Human
Note

abm's miRNA All-In-One cDNA Synthesis Kit (Cat No. G898) must be used to reverse transcribe the RNA. Failure to do so will not yield valid qPCR results. Each primer set includes a universal reverse primer and is specific for the unique and proprietary sequence incorporated into the cDNA by the miRNA adapter in the miRNA cDNA synthesis kit. The miRNA qPCR primer sets cannot accurately detect cDNA generated using other first strand synthesis kits.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. MPH00001
Print & Download Datasheet
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  • Zhou, Y., Lei, J., Xie, Q., Wu, L., Jin, S., Guo, B., ... & Zhang, J. "Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5" Science Advances 5(11):eaax0629 (2019).
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  • Alexis Autour, Sunny C. Y. Jeng, Adam D. Cawte, Amir Abdolahzadeh, Angela Galli, Shanker S. S. Panchapakesan, David Rueda, Michael Ryckelynck & Peter J. Unrau "Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells" Nature Communications. 2018 Feb;23(9):656. DOI: 10.1038/s41467-018-02993-8.
  • Panchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. et al. "Ribonucleoprotein purification and characterization using RNA Mango." RNA. 2017 Oct;23(10):1592-1599. DOI: 10.1261/rna.062166.117.
  • Trachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferré-D'Amaré AR. et al. "Structural basis for high-affinity fluorophore binding and activation by RNA Mango." Nat Chem Biol. 2017 Jul;13(7):807-813. DOI: 10.1038/nchembio.2392.
  • Cawte, Adam. et al. "Live Cell Imaging of Genomic Loci using Fluorescent RNA Aptamers." Biophysical Journal. 2017 Feb, Volume 112,Issue 3,69a. DOI: 10.1016/j.bpj.2016.11.416.
  • Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al. "Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers." RNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24. PubMed : 27777365.
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  • Dolgosheina EV, Jeng SC, Panchapakesan SS, Cojocaru R, Chen PS, Wilson PD, Hawkins N, Wiggins PA, Unrau PJ. et al. "RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking." ACS Chem Biol. 2014 Oct 17;9(10):2412-20. DOI: 10.1021/cb500499x. Epub 2014 Aug 21.
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