iLenti-siRNA
| Vector Name | iLenti-siRNA |
| VectorType | Lentiviral vector |
| Antibiotic Information | Kanamycin for bacterial selection and puromycin for mammalian selection. |
| Sequencing Primers | 5'---CAACCCGCTCCAAGGAATCG---3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggcta gctctagata cgtagaacgc tgacgtcatc
1861 aacccgctcc aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc
1921 gccctggcag gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt
1981 cgctatgtgt tctgggaaat caccataaac gtgaaatgtc tttggatttg ggaatcttat
2041 aagttctgta tgagaccaca aaaatagtct tcgatatcat ttaaatgtcg acagaagaca
2101 gctttttttg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt
2161 tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta
2221 cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt
2281 caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat
2341 catgggaaat aggccctctt cctgcccgac cttggctcga gcaattgtag taatcaatta
2401 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
2461 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
2521 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
2581 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
2641 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
2701 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctggttt agtgaaccgt cagggtaccg atatcactag tggatccatg gccaccgagt
3001 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
3061 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
3121 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
3181 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
3241 cgggggcggt gttcgccgag atcggctcgc gcatggccga gttgagcggt tcccggctgg
3301 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
3361 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
3421 tgctccccgg agtggaggcg gccgagcgcg ctggggtgcc cgccttcctg gagacctccg
3481 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
3541 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg
3601 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
3661 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
3721 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
3781 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
3841 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
3901 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
3961 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
4021 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
4081 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
4141 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat
4201 ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct
4261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
4321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
4381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
4441 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
4501 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
4561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
4621 atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat
4681 gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa ataccgcaca
4741 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
4801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
4861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
4921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
4981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
5401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5581 agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa aggatcttca
5641 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
5701 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
5761 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
5821 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
5881 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
5941 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
6001 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
6061 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
6121 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
6181 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
6241 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
6301 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
6361 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
6421 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
6481 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
6541 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
6601 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
6661 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
6721 tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca
6781 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
6841 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
6901 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
6961 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
7021 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
7081 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
7141 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat
7201 taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca atatgataat
7261 gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt
7321 gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa
7381 agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag
7441 gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa
7501 ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatactctc
7561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
7621 tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg aaaatgtagt
7681 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
7741 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gat
LOCUS dna 7793 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 1833..1838
/dbxref="REBASE:XbaI"
misc_binding 1881..1886
/dbxref="REBASE:ApaI"
Promoter 2117..2373
/gene="hU6 prom"
misc_binding 2376..2381
/dbxref="REBASE:XhoI"
ORF 2901..3590
/sequence="ORF_2 rf(3)"
misc_binding 2964..2969
/dbxref="REBASE:KpnI"
misc_binding 2982..2987
/dbxref="REBASE:BamHI"
Marker 2994..3590
/gene="puro marker"
misc_binding 3040..3045
/dbxref="REBASE:SmaI"
misc_binding 3040..3045
/dbxref="REBASE:XmaI"
misc_binding 3320..3325
/dbxref="REBASE:StuI"
Other Gene 4200..4252
/gene="delta_U3 other"
Other Gene 4253..4433
/gene="HIV-1_5_LTR other"
Rep_Origin 4940..5559
/gene="pBR322 origin"
Promoter 5817..5866
/gene="NEOKAN prom"
misc_binding 5924..5929
/dbxref="REBASE:BclI"
ORF 5955..6749
/sequence="ORF_3 rf(3)"
Marker 5958..6746
/gene="NTP_II marker"
Rep_Origin 6854..7160
/gene="f1 origin"
Other Gene 7215..7316
/gene="RITR other"
Other Gene 7215..7316
/gene="LITR other"
Other Gene 7396..7544
/gene="Encap other"
BASE COUNT 1986 a 1863 c 2110 g 1834 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggcta gctctagata cgtagaacgc tgacgtcatc
1861 aacccgctcc aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc
1921 gccctggcag gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt
1981 cgctatgtgt tctgggaaat caccataaac gtgaaatgtc tttggatttg ggaatcttat
2041 aagttctgta tgagaccaca aaaatagtct tcgatatcat ttaaatgtcg acagaagaca
2101 gctttttttg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt
2161 tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta
2221 cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt
2281 caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat
2341 catgggaaat aggccctctt cctgcccgac cttggctcga gcaattgtag taatcaatta
2401 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
2461 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
2521 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
2581 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
2641 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
2701 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctggttt agtgaaccgt cagggtaccg atatcactag tggatccatg gccaccgagt
3001 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
3061 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
3121 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
3181 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
3241 cgggggcggt gttcgccgag atcggctcgc gcatggccga gttgagcggt tcccggctgg
3301 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
3361 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
3421 tgctccccgg agtggaggcg gccgagcgcg ctggggtgcc cgccttcctg gagacctccg
3481 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
3541 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg
3601 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
3661 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
3721 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
3781 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
3841 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
3901 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
3961 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
4021 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
4081 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
4141 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat
4201 ggaagggcta attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct
4261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
4321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
4381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
4441 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
4501 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
4561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
4621 atcttatcat gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat
4681 gggtattatg ggtctgcatt aatgaatcgg ccaacggatg tcggtgtgaa ataccgcaca
4741 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
4801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
4861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
4921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
4981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
5401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5581 agtggaacga aaactcacgt taagggattt tggccatgag attatcaaaa aggatcttca
5641 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
5701 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
5761 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
5821 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
5881 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
5941 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
6001 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
6061 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
6121 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
6181 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
6241 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
6301 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
6361 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
6421 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
6481 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
6541 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
6601 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
6661 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
6721 tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca
6781 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
6841 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
6901 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
6961 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
7021 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
7081 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
7141 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat
7201 taattcttaa ttaacatcat caataatata ccttttttgg attgaagcca atatgataat
7261 gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt
7321 gtggcggaag tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa
7381 agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag
7441 gcggatgttg tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa
7501 ctgaataaga ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatactctc
7561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
7621 tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg aaaatgtagt
7681 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
7741 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gat
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
XbaI
|
1801 aaattttcgggtttttcgaacctagggctagctctagatacgtagaacgctgacgtcatc 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCGATCGAGATCTATGCATCTTGCGACTGCAGTAG
ApaI
|
1861 aacccgctccaaggaatcgcgggcccagtgtcactaggcgggaacacccagcgcgcgtgc 1920
TTGGGCGAGGTTCCTTAGCGCCCGGGTCACAGTGATCCGCCCTTGTGGGTCGCGCGCACG
1921 gccctggcaggaagatggctgtgagggacaggggagtggcgccctgcaatatttgcatgt 1980
CGGGACCGTCCTTCTACCGACACTCCCTGTCCCCTCACCGCGGGACGTTATAAACGTACA
1981 cgctatgtgttctgggaaatcaccataaacgtgaaatgtctttggatttgggaatcttat 2040
GCGATACACAAGACCCTTTAGTGGTATTTGCACTTTACAGAAACCTAAACCCTTAGAATA
2041 aagttctgtatgagaccacaaaaatagtcttcgatatcatttaaatgtcgacagaagaca 2100
TTCAAGACATACTCTGGTGTTTTTATCAGAAGCTATAGTAAATTTACAGCTGTCTTCTGT
hU6 prom(2117,2373)<<<
|
2101 gctttttttggtgtttcgtcctttccacaagatatataaagccaagaaatcgaaatactt 2160
CGAAAAAAACCACAAAGCAGGAAAGGTGTTCTATATATTTCGGTTCTTTAGCTTTATGAA
2161 tcaagttacggtaagcatatgatagtccattttaaaacataattttaaaactgcaaacta 2220
AGTTCAATGCCATTCGTATACTATCAGGTAAAATTTTGTATTAAAATTTTGACGTTTGAT
2221 cccaagaaattattactttctacgtcacgtattttgtactaatatctttgtgtttacagt 2280
GGGTTCTTTAATAATGAAAGATGCAGTGCATAAAACATGATTATAGAAACACAAATGTCA
2281 caaattaattctaattatctctctaacagccttgtatcgtatatgcaaatatgaaggaat 2340
GTTTAATTAAGATTAATAGAGAGATTGTCGGAACATAGCATATACGTTTATACTTCCTTA
XhoI
|
2341 catgggaaataggccctcttcctgcccgaccttggctcgagcaattgtagtaatcaatta 2400
GTACCCTTTATCCGGGAGAAGGACGGGCTGGAACCGAGCTCGTTAACATCATTAGTTAAT
2401 cggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatg 2460
GCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTGAATGCCATTTAC
2461 gcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttc 2520
CGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAG
2521 ccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaa 2580
GGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTT
2581 ctgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtca 2640
GACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGT
2641 atgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttccta 2700
TACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGAT
2701 cttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagt 2760
GAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_2 rf(3)(2901,3590)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
KpnI BamHI puro marker(2994,3590)>>>
| | |
2941 gagctggtttagtgaaccgtcagggtaccgatatcactagtggatccatggccaccgagt 3000
CTCGACCAAATCACTTGGCAGTCCCATGGCTATAGTGATCACCTAGGTACCGGTGGCTCA
SmaI
|
XmaI
| |
3001 acaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcg 3060
TGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGAGC
3061 ccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcg 3120
GGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTAGC
3121 agcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaagg 3180
TCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCC
3181 tgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaag 3240
ACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTC
3241 cgggggcggtgttcgccgagatcggctcgcgcatggccgagttgagcggttcccggctgg 3300
GCCCCCGCCACAAGCGGCTCTAGCCGAGCGCGTACCGGCTCAACTCGCCAAGGGCCGACC
StuI
|
3301 ccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggt 3360
GGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCA
3361 tcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcg 3420
AGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGC
3421 tgctccccggagtggaggcggccgagcgcgctggggtgcccgccttcctggagacctccg 3480
ACGAGGGGCCTCACCTCCGCCGGCTCGCGCGACCCCACGGGCGGAAGGACCTCTGGAGGC
3481 cgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgagg 3540
GCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCC
3541 tgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccg 3600
ACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGC
3601 gaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttg 3660
CTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAAC
3661 ctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttccc 3720
GAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGG
3721 gtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagt 3780
CATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCA
3781 tgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaaccccca 3840
ACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGT
3841 ctggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctcc 3900
GACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGG
3901 ctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggc 3960
GATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCG
3961 tgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgc 4020
ACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACG
4021 tcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccc 4080
AGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGG
4081 tcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtc 4140
AGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAG
4141 tcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggat 4200
AGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTA
delta_U3 other(4200,4252)>>> HIV-1_5_LTR other(4253,4433)>>>
| |
4201 ggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactgggtctct 4260
CCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGA
4261 ctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaa 4320
GACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATT
4321 gcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactc 4380
CGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAG
4381 tggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtag 4440
ACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATC
4441 ttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtga 4500
AAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACT
4501 gaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaattt 4560
CTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAA
4561 cacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgt 4620
GTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACA
4621 atcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattat 4680
TAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATA
4681 gggtattatgggtctgcattaatgaatcggccaacggatgtcggtgtgaaataccgcaca 4740
CCCATAATACCCAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGT
4741 gatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgc 4800
CTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCG
4801 tgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt 4860
ACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCA
4861 tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaagg 4920
ATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCC
pBR322 origin(4940,5559)<<<
|
4921 ccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacg 4980
GGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGC
4981 agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagat 5040
TCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTA
5041 accaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctta 5100
TGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAAT
5101 ccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgct 5160
GGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGA
5161 gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacccc 5220
CATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGG
5221 ccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaa 5280
GGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATT
5281 gacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatg 5340
CTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATAC
5341 taggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacag 5400
ATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTC
5401 tatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctt 5460
ATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAA
5461 gatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagatta 5520
CTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAAT
5521 cgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc 5580
GCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAG
5581 agtggaacgaaaactcacgttaagggattttggccatgagattatcaaaaaggatcttca 5640
TCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGT
5641 cctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatg 5700
GGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTAC
5701 tcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagctt 5760
AGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAA
NEOKAN prom(5817,5866)>>>
|
5761 gcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccgg 5820
CGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCC
5821 aattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatgg 5880
TTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACC
BclI
|
5881 ctttcttgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggat 5940
GAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTA
NTP_II marker(5958,6746)>>>
|
ORF_3 rf(3)(5955,6749)>>>
| |
5941 gaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttggg 6000
CTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCC
6001 tggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccg 6060
ACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGC
6061 tgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtg 6120
ACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCAC
6121 ccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttc 6180
GGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAG
6181 cttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcg 6240
GAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGC
6241 aagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatca 6300
TTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGT
6301 tggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccacc 6360
ACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGG
6361 aagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcagg 6420
TTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCC
6421 atgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaagg 6480
TACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCC
6481 cgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaata 6540
GCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTAT
6541 tcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcgg 6600
AGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCC
6601 accgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaat 6660
TGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTA
6661 gggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgcct 6720
CCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGA
6721 tctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctca 6780
AGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGT
6781 ttttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgag 6840
AAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTC
f1 origin(6854,7160)<<<
|
6841 atagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactcc 6900
TATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGG
6901 aacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccc 6960
TTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGG
6961 taatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagc 7020
ATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCG
7021 ccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaa 7080
GGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTT
7081 gcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccacc 7140
CGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGG
7141 acacccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaat 7200
TGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTA
RITR other(7215,7316)<<<
|
LITR other(7215,7316)>>>
|
7201 taattcttaattaacatcatcaataatataccttttttggattgaagccaatatgataat 7260
ATTAAGAATTAATTGTAGTAGTTATTATATGGAAAAAACCTAACTTCGGTTATACTATTA
7261 gagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagt 7320
CTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCA
7321 gtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaa 7380
CACCGCCTTCACACTACAACGTTCACACCGCCTTGTGTACATTCGCTGCCTACACCGTTT
Encap other(7396,7544)>>>
|
7381 agtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttag 7440
TCACTGCAAAAACCACACGCGGCCACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATC
7441 gcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcgggaaaa 7500
CGCCTACAACATCATTTAAACCCGCATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTT
7501 ctgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatactctc 7560
GACTTATTCTCCTTCACTTTAGACTTATTAAAACACAATGAGTATCGCGCATTATGAGAG
7561 atgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca 7620
TACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGT
7621 tttccccgaaaagtgccacctgacgtaactataacggtcctaaggtagcgaaaatgtagt 7680
AAAGGGGCTTTTCACGGTGGACTGCATTGATATTGCCAGGATTCCATCGCTTTTACATCA
7681 cttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttac 7740
GAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATG
7741 aaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgat 7793
TTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 7793 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 1833..1838
/dbxref="REBASE:XbaI"
misc_binding 1881..1886
/dbxref="REBASE:ApaI"
Promoter 2117..2373
/gene="hU6 prom"
misc_binding 2376..2381
/dbxref="REBASE:XhoI"
ORF 2901..3590
/sequence="ORF_2 rf(3)"
misc_binding 2964..2969
/dbxref="REBASE:KpnI"
misc_binding 2982..2987
/dbxref="REBASE:BamHI"
Marker 2994..3590
/gene="puro marker"
misc_binding 3040..3045
/dbxref="REBASE:SmaI"
misc_binding 3040..3045
/dbxref="REBASE:XmaI"
misc_binding 3320..3325
/dbxref="REBASE:StuI"
Other Gene 4200..4252
/gene="delta_U3 other"
Other Gene 4253..4433
/gene="HIV-1_5_LTR other"
Rep_Origin 4940..5559
/gene="pBR322 origin"
Promoter 5817..5866
/gene="NEOKAN prom"
misc_binding 5924..5929
/dbxref="REBASE:BclI"
ORF 5955..6749
/sequence="ORF_3 rf(3)"
Marker 5958..6746
/gene="NTP_II marker"
Rep_Origin 6854..7160
/gene="f1 origin"
Other Gene 7215..7316
/gene="RITR other"
Other Gene 7215..7316
/gene="LITR other"
Other Gene 7396..7544
/gene="Encap other"
BASE COUNT 1986 a 1863 c 2110 g 1834 t 0 others
ORIGIN