pAAV-G-MSCV-2A-Luc-Blank
| Vector Name | pAAV-G-MSCV-2A-Luc-Blank |
| VectorType | AAV Plasmid |
| Antibiotic Information | Bacterial: Kanamycin |
| Sequencing Primers | MSCV sequencing primer 5'--GCC CAC AAC CCC TCA CTT--3' |
| Additional Information | The blank expression is driven by a MSCV promoter, with a Luciferase reporter (not fusion). Vector size: 6189bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggtg
661 aaagacccca cctgtaggtt tggcaagtta gcttaagtaa cgccattttg caaggcatgg
721 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
781 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
841 agatggtccc cagatgcggt cccgccctca gcagtttcta gcgaaccatc agatgtttcc
901 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagtttgc
961 ttcttgcttc tgtttgtgtg cttctgctcc ctgagctcaa taaaagagcc cacaacccct
1021 cacttggtgg gccagtcctc tgatagactg tgtcccctgg atacccgtat gctagcgata
1081 tcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctgaat
1141 tcatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
1201 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
1261 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
1321 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
1381 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
1441 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
1501 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
1561 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
1621 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
1681 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
1741 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
1801 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
1861 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
1921 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
1981 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
2041 tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca
2101 gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct
2161 acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg
2221 gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta
2281 agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg
2341 gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca
2401 gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga
2461 gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc
2521 aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc
2581 tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg
2641 actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg
2701 acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca
2761 ttaaggccaa gaagggcggc aagatcgccg tgtaactcga gtacccatac gacgtcccag
2821 actacgctac gcgtcttaag gcgatcgcag acatgataag atacattgat gagtttggac
2881 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
2941 ctttatttgt aaccattata agctgcaata aacaagttaa taacaacaat tccattcatt
3001 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
3061 aatgtggtag tcgaaattcc cgataaggat cttcctagag catggctacg tagataagta
3121 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc
3181 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
3241 tgcccgggcg gcctcagtga gcgagcgagc gcgcagggat cccggtgtga aataccgcac
3301 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
3361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
3661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
3721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
3781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
3841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
3901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
3961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
4021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
4081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
4141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
4201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
4261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
4321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
4381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
4441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
4501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
4561 aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag
4621 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
4681 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
4741 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
4801 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat
4861 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
4921 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
4981 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
5041 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
5101 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
5161 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
5221 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
5281 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
5341 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
5401 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
5461 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
5521 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
5581 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
5641 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
5701 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
5761 ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt
5821 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
5881 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg
5941 tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
6001 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
6061 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc
6121 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata
6181 atatacctt
LOCUS dna 6189 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 993..998
/dbxref="REBASE:SacI"
misc_binding 1137..1142
/dbxref="REBASE:EcoRI"
ORF 1143..2795
/sequence="ORF_1 rf(3)"
misc_binding 1169..1174
/dbxref="REBASE:ApaI"
misc_binding 2796..2801
/dbxref="REBASE:XhoI"
misc_binding 3277..3282
/dbxref="REBASE:BamHI"
Rep_Origin 3501..4120
/gene="pBR322 origin"
Promoter 4773..4822
/gene="NEOKAN prom"
ORF 4911..5705
/sequence="ORF_2 rf(3)"
Marker 4914..5702
/gene="NTP_II marker"
misc_binding 5440..5445
/dbxref="REBASE:SphI"
misc_binding 5471..5476
/dbxref="REBASE:NcoI"
Rep_Origin 5810..6115
/gene="f1 origin"
BASE COUNT 1519 a 1614 c 1702 g 1354 t 0 others
ORIGIN
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG
481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540
TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC
541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600
TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT
601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggtg 660
TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCAC
661 aaagaccccacctgtaggtttggcaagttagcttaagtaacgccattttgcaaggcatgg 720
TTTCTGGGGTGGACATCCAAACCGTTCAATCGAATTCATTGCGGTAAAACGTTCCGTACC
721 aaaatacataactgagaatagagaagttcagatcaaggttaggaacagagagacagcaga 780
TTTTATGTATTGACTCTTATCTCTTCAAGTCTAGTTCCAATCCTTGTCTCTCTGTCGTCT
781 atatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaac 840
TATACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCCGAGTCCCGGTTCTTG
841 agatggtccccagatgcggtcccgccctcagcagtttctagcgaaccatcagatgtttcc 900
TCTACCAGGGGTCTACGCCAGGGCGGGAGTCGTCAAAGATCGCTTGGTAGTCTACAAAGG
901 agggtgccccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagtttgc 960
TCCCACGGGGTTCCTGGACTTTACTGGGACACGGAATAAACTTGATTGGTTAGTCAAACG
SacI
|
961 ttcttgcttctgtttgtgtgcttctgctccctgagctcaataaaagagcccacaacccct 1020
AAGAACGAAGACAAACACACGAAGACGAGGGACTCGAGTTATTTTCTCGGGTGTTGGGGA
1021 cacttggtgggccagtcctctgatagactgtgtcccctggatacccgtatgctagcgata 1080
GTGAACCACCCGGTCAGGAGACTATCTGACACAGGGGACCTATGGGCATACGATCGCTAT
EcoRI
|
1081 tcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctgaat 1140
AGCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACCTCCTCTTAGGGCCGGGACTTA
ORF_1 rf(3)(1143,2795)>>> ApaI
| |
1141 tcatggaagatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacg 1200
AGTACCTTCTACGGTTTTTGTAATTCTTCCCGGGTCGCGGTAAGATGGGTGAGCTTCTGC
1201 ggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccggcacca 1260
CCTGGCGGCCGCTCGTCGACGTGTTTCGGTACTTCGCGATGCGGGACCACGGGCCGTGGT
1261 tcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtacttcgagatga 1320
AGCGGAAATGGCTGCGTGTATAGCTCCACCTGTAATGGATGCGGCTCATGAAGCTCTACT
1321 gcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtgg 1380
CGCAAGCCGACCGTCTTCGATACTTCGCGATACCCGACTTATGTTTGGTAGCCTAGCACC
1381 tgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtg 1440
ACACGTCGCTCTTATCGAACGTCAAGAAGTACGGGCACAACCCACGGGACAAGTAGCCAC
1441 tggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggca 1500
ACCGACACCGGGGTCGATTGCTGTAGATGTTGCTCGCGCTCGACGACTTGTCGTACCCGT
1501 tcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgc 1560
AGTCGGTCGGGTGGCAGCATAAGCACTCGTTCTTTCCCGACGTTTTCTAGGAGTTGCACG
1561 aaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagg 1620
TTTTCTTCGATGGCTAGTATGTTTTCTAGTAGTAGTACCTATCGTTCTGGCTGATGGTCC
1621 gcttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacg 1680
CGAAGGTTTCGTACATGTGGAAGCACTGAAGGGTAAACGGTGGGCCGAAGTTGCTCATGC
1681 acttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatcatgaacagtagtg 1740
TGAAGCACGGGCTCTCGAAGCTGGCCCTGTTTTGGTAGCGGGACTAGTACTTGTCATCAC
1741 gcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattca 1800
CGTCATGGCCTAACGGGTTCCCGCATCGGGATGGCGTGGCGTGGCGAACACAGGCTAAGT
1801 gtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcg 1860
CAGTACGGGCGCTGGGGTAGAAGCCGTTGGTCTAGTAGGGGCTGTGGCGATAGGAGTCGC
1861 tggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggct 1920
ACCACGGTAAAGTGGTGCCGAAGCCGTACAAGTGGTGCGACCCGATGAACTAGACGCCGA
1921 ttcgggtcgtgctcatgtaccgcttcgaggaggagctattcttgcgcagcttgcaagact 1980
AAGCCCAGCACGAGTACATGGCGAAGCTCCTCCTCGATAAGAACGCGTCGAACGTTCTGA
1981 ataagattcaatctgccctgctggtgcccacactatttagcttcttcgctaagagcactc 2040
TATTCTAAGTTAGACGGGACGACCACGGGTGTGATAAATCGAAGAAGCGATTCTCGTGAG
2041 tcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgctca 2100
AGTAGCTGTTCATGCTGGATTCGTTGAACGTGCTCTAGCGGTCGCCGCCCCGCGGCGAGT
2101 gcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggct 2160
CGTTCCTCCATCCACTCCGGCACCGGTTTGCGAAGGTGGATGGTCCGTAGGCGGTCCCGA
2161 acggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagcctg 2220
TGCCGGACTGTCTTTGTTGGTCGCGGTAAGACTAGTGGGGGCTTCCCCTGCTGTTCGGAC
2221 gcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggta 2280
CGCGTCATCCGTTCCACCACGGGAAGAAGCTCCGATTCCACCACCTGAACCTGTGGCCAT
2281 agacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcg 2340
TCTGTGACCCACACTTGGTCGCGCCGCTCGACACGCAGGCACCGGGGTACTAGTACTCGC
2341 gctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcaca 2400
CGATGCAATTGTTGGGGCTCCGATGTTTGCGAGAGTAGCTGTTCCTGCCGACCGACGTGT
2401 gcggcgacatcgcctactgggacgaggacgagcacttcttcatcgtggaccggctgaaga 2460
CGCCGCTGTAGCGGATGACCCTGCTCCTGCTCGTGAAGAAGTAGCACCTGGCCGACTTCT
2461 gcctgatcaaatacaagggctaccaggtagccccagccgaactggagagcatcctgctgc 2520
CGGACTAGTTTATGTTCCCGATGGTCCATCGGGGTCGGCTTGACCTCTCGTAGGACGACG
2521 aacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgccggcgagc 2580
TTGTGGGGTTGTAGAAGCTGCGGCCCCAGCGGCCGGACGGGCTGCTGCTACGGCCGCTCG
2581 tgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtgg 2640
ACGGGCGGCGTCAGCAGCACGACCTTGTGCCATTTTGGTACTGGCTCTTCCTCTAGCACC
2641 actatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtgg 2700
TGATACACCGGTCGGTCCAATGTTGGCGGTTCTTCGACGCGCCACCACAACACAAGCACC
2701 acgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctca 2760
TGCTCCACGGATTTCCTGACTGGCCGTTCAACCTGCGGGCGTTCTAGGCGCTCTAAGAGT
XhoI
|
2761 ttaaggccaagaagggcggcaagatcgccgtgtaactcgagtacccatacgacgtcccag 2820
AATTCCGGTTCTTCCCGCCGTTCTAGCGGCACATTGAGCTCATGGGTATGCTGCAGGGTC
2821 actacgctacgcgtcttaaggcgatcgcagacatgataagatacattgatgagtttggac 2880
TGATGCGATGCGCAGAATTCCGCTAGCGTCTGTACTATTCTATGTAACTACTCAAACCTG
2881 aaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattg 2940
TTTGGTGTTGATCTTACGTCACTTTTTTTACGAAATAAACACTTTAAACACTACGATAAC
2941 ctttatttgtaaccattataagctgcaataaacaagttaataacaacaattccattcatt 3000
GAAATAAACATTGGTAATATTCGACGTTATTTGTTCAATTATTGTTGTTAAGGTAAGTAA
3001 ttatgtttcaggttcagggggagatgtgggaggttttttaaagcaagtaaaacctctaca 3060
AATACAAAGTCCAAGTCCCCCTCTACACCCTCCAAAAAATTTCGTTCATTTTGGAGATGT
3061 aatgtggtagtcgaaattcccgataaggatcttcctagagcatggctacgtagataagta 3120
TTACACCATCAGCTTTAAGGGCTATTCCTAGAAGGATCTCGTACCGATGCATCTATTCAT
3121 gcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctc 3180
CGTACCGCCCAATTAGTAATTGATGTTCCTTGGGGATCACTACCTCAACCGGTGAGGGAG
3181 tctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctt 3240
AGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAA
BamHI
|
3241 tgcccgggcggcctcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgcac 3300
ACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCGTG
3301 agatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcg 3360
TCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGC
3361 ctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacgg 3420
GACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCC
3421 ttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaag 3480
AATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTC
pBR322 origin(3501,4120)<<<
|
3481 gccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgac 3540
CGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTG
3541 gagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaaga 3600
CTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCT
3601 taccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctt 3660
ATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAA
3661 accggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgc 3720
TGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCG
3721 tgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccc 3780
ACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGG
3781 cccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggta 3840
GGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCAT
3841 agacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtat 3900
TCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATA
3901 gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggaca 3960
CATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGT
3961 gtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctct 4020
CATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGA
4021 tgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagatt 4080
ACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAA
4081 acgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgct 4140
TGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGA
4141 cagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttc 4200
GTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAG
4201 acctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaa 4260
TGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATT
4261 acttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtcta 4320
TGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGAT
4321 tttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggc 4380
AAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCG
4381 ttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagat 4440
AATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTA
4441 ttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttta 4500
AATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAAT
4501 tccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtt 4560
AGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAA
4561 aatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaag 4620
TTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTC
4621 ccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaa 4680
GGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTT
4681 gggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagc 4740
CCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCG
NEOKAN prom(4773,4822)>>>
|
4741 tagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctg 4800
ATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGAC
4801 gtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgat 4860
CATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTA
NTP_II marker(4914,5702)>>>
|
ORF_2 rf(3)(4911,5705)>>>
| |
4861 ggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaac 4920
CCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTG
4921 aagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgact 4980
TTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGA
4981 gggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggc 5040
CCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCG
5041 gcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgagg 5100
CGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCC
5101 cagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttg 5160
GTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAAC
5161 tcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgt 5220
AGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACA
5221 catctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgc 5280
GTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACG
5281 atacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgag 5340
TATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTC
5341 cacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcagg 5400
GTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCC
SphI
|
5401 ggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatc 5460
CCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAG
NcoI
|
5461 tcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctttt 5520
AGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAA
5521 ctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttgg 5580
GACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACC
5581 ctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgcttt 5640
GATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAA
5641 acggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttct 5700
TGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGA
5701 tctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatc 5760
AGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAG
f1 origin(5810,6115)<<<
|
5761 ggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtt 5820
CCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAA
5821 tggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtc 5880
ACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAG
5881 tatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgagg 5940
ATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCC
5941 tgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacgggga 6000
ACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCT
6001 aagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcg 6060
TTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGC
6061 ctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgc 6120
GACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCG
6121 tacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaata 6180
ATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTAT
6181 atatacctt 6189
TATATGGAA