pAAV-ScrambledsiRNA-GFP-hGH-amp
| Vector Name | pAAV-ScrambledsiRNA-GFP-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Amp/Puro |
| Sequencing Primers | U6 promoter 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggtacgtc caaggtcggg caggaagagg gcctatttcc
181 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
241 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
301 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
361 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacaaa aagggtgaac
421 tcacgtcaga attttttggt accctttttt tggtgtttcg tggtctcata cagaacttat
481 aagattccca aatccaaaga catttcacgt ttatggtgat ttcccagaac acatagcgac
541 atgcaaatat tgcagggcgc cactcccctg tccctcacag ccatcttcct gccagggcgc
601 acgcgcgctg ggtgttcccg cctagtgaca ctgggcccgc gattccttgg agcgggttga
661 tgacgtcagc gttctcaatt gtagtaatca attacggggt cattagttca tagcccatat
721 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
781 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
841 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
901 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
961 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
1021 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
1081 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
1141 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
1201 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagggt
1261 accggatccc ttaagtgaac aactagtgcc accatggtga gcaagggcga ggagctgttc
1321 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
1381 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
1441 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
1501 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
1561 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
1621 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
1681 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
1741 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
1801 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
1861 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
1921 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
1981 atcactctcg gcatggacga gctgtacaag gaattcggaa gcgggcagtg caccaactac
2041 gccctgctga agctggccgg cgacgtggag agcaaccccg gccccggatc catggccacc
2101 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
2161 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
2221 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
2281 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
2341 gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag cggttcccgg
2401 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
2461 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
2521 gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt cctggagacc
2581 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
2641 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt
2701 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
2761 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
2821 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaga
2881 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
2941 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
3001 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
3061 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
3121 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttcac
3181 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
3241 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
3301 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
3361 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
3421 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
3481 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
3541 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
3601 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
3661 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
3721 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
3781 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
3841 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
3901 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
3961 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
4021 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagccg ggagctgcat gtgtcagagg
4081 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
4141 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
4201 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
4261 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
4321 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
4381 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
4441 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
4501 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
4561 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
4621 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
4681 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
4741 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
4801 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
4861 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
4921 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
4981 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
5041 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
5101 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
5161 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
5221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
5281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
5461 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
5521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
5581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
5641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
5701 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
5761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
5821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
5881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
5941 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
LOCUS dna 5989 bp
FEATURES Location/Qualifiers
Promoter 152..407
/gene="hU6 prom"
misc_binding 633..638
/dbxref="REBASE:ApaI"
ORF 1243..2709
/sequence="ORF_3 rf(5)"
Reporter 1294..2010
/gene="EGFP reporter"
ORF 1294..2694
/sequence="ORF_1 rf(1)"
Marker 2098..2694
/gene="puro marker"
misc_binding 2203..2208
/dbxref="REBASE:HincII"
misc_binding 2203..2208
/dbxref="REBASE:AccI"
misc_binding 2424..2429
/dbxref="REBASE:StuI"
Terminator 2701..3177
/gene="hGH_PA term"
misc_binding 3013..3018
/dbxref="REBASE:SphI"
Rep_Origin 3432..3738
/gene="f1 origin"
Promoter 4239..4267
/gene="amp prom"
Marker 4309..5169
/gene="amp marker"
ORF 4309..5169
/sequence="ORF_2 rf(1)"
Rep_Origin 5324..5943
/gene="pBR322 origin"
misc_binding 5570..5578
/dbxref="REBASE:AlwNI"
BASE COUNT 1353 a 1665 c 1590 g 1381 t 0 others
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggtacgtc caaggtcggg caggaagagg gcctatttcc
181 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
241 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
301 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
361 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacaaa aagggtgaac
421 tcacgtcaga attttttggt accctttttt tggtgtttcg tggtctcata cagaacttat
481 aagattccca aatccaaaga catttcacgt ttatggtgat ttcccagaac acatagcgac
541 atgcaaatat tgcagggcgc cactcccctg tccctcacag ccatcttcct gccagggcgc
601 acgcgcgctg ggtgttcccg cctagtgaca ctgggcccgc gattccttgg agcgggttga
661 tgacgtcagc gttctcaatt gtagtaatca attacggggt cattagttca tagcccatat
721 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
781 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
841 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
901 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
961 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
1021 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
1081 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
1141 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
1201 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagggt
1261 accggatccc ttaagtgaac aactagtgcc accatggtga gcaagggcga ggagctgttc
1321 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
1381 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
1441 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
1501 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
1561 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
1621 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
1681 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
1741 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
1801 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
1861 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
1921 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
1981 atcactctcg gcatggacga gctgtacaag gaattcggaa gcgggcagtg caccaactac
2041 gccctgctga agctggccgg cgacgtggag agcaaccccg gccccggatc catggccacc
2101 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
2161 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
2221 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
2281 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
2341 gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag cggttcccgg
2401 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
2461 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
2521 gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt cctggagacc
2581 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
2641 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt
2701 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
2761 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
2821 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaga
2881 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
2941 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
3001 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
3061 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
3121 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttcac
3181 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
3241 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
3301 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
3361 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
3421 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
3481 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
3541 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
3601 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
3661 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
3721 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
3781 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
3841 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
3901 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
3961 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
4021 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagccg ggagctgcat gtgtcagagg
4081 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
4141 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
4201 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
4261 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
4321 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
4381 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
4441 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
4501 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
4561 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
4621 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
4681 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
4741 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
4801 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
4861 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
4921 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
4981 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
5041 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
5101 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
5161 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
5221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
5281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
5461 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
5521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
5581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
5641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
5701 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
5761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
5821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
5881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
5941 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
LOCUS dna 5989 bp
FEATURES Location/Qualifiers
Promoter 152..407
/gene="hU6 prom"
misc_binding 633..638
/dbxref="REBASE:ApaI"
ORF 1243..2709
/sequence="ORF_3 rf(5)"
Reporter 1294..2010
/gene="EGFP reporter"
ORF 1294..2694
/sequence="ORF_1 rf(1)"
Marker 2098..2694
/gene="puro marker"
misc_binding 2203..2208
/dbxref="REBASE:HincII"
misc_binding 2203..2208
/dbxref="REBASE:AccI"
misc_binding 2424..2429
/dbxref="REBASE:StuI"
Terminator 2701..3177
/gene="hGH_PA term"
misc_binding 3013..3018
/dbxref="REBASE:SphI"
Rep_Origin 3432..3738
/gene="f1 origin"
Promoter 4239..4267
/gene="amp prom"
Marker 4309..5169
/gene="amp marker"
ORF 4309..5169
/sequence="ORF_2 rf(1)"
Rep_Origin 5324..5943
/gene="pBR322 origin"
misc_binding 5570..5578
/dbxref="REBASE:AlwNI"
BASE COUNT 1353 a 1665 c 1590 g 1381 t 0 others