pLenti-E1A-E1B
| Vector Name | pLenti-E1A-E1B |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcagc caccatgaga catattatct gccacggagg tgttattacc gaagaaatgg
3121 ccgccagtct tttggaccag ctgatcgaag aggtactggc tgataatctt ccacctccta
3181 gccattttga accacctacc cttcacgaac tgtatgattt agacgtgacg gcccccgaag
3241 atcccaacga ggaggcggtt tcgcagattt ttcccgactc tgtaatgttg gcggtgcagg
3301 aagggattga cttactcact tttccgccgg cgcccggttc tccggagccg cctcaccttt
3361 cccggcagcc cgagcagccg gagcagagag ccttgggtcc ggtttctatg ccaaaccttg
3421 taccggaggt gatcgatctt acctgccacg aggctggctt tccacccagt gacgacgagg
3481 atgaagaggg tcctgtgtct gaacctgagc ctgagcccga gccagaaccg gagcctgcaa
3541 gacctacccg ccgtcctaaa atggcgcctg ctatcctgag acgcccgaca tcacctgtgt
3601 ctagagaatg caatagtagt acggatagct gtgactccgg tccttctaac acacctcctg
3661 agatacaccc ggtggtcccg ctgtgcccca ttaaaccagt tgccgtgaga gttggtgggc
3721 gtcgccaggc tgtggaatgt atcgaggact tgcttaacga gcctgggcaa cctttggact
3781 tgagctgtaa acgccccagg ccagagggca gaggaagtct tctaacatgc ggtgacgtgg
3841 aggagaatcc cggccctatg gaggcttggg agtgtttgga agatttttct gctgtgcgta
3901 acttgctgga acagagctct aacagtacct cttggttctg gaggtttctg tggggctcat
3961 cccaggcaaa gttagtctgc agaattaagg aggattacaa gtgggaattc gaagagcttt
4021 tgaagtcctg tggtgagctg tttgattctt tgaatctggg tcaccaggcg cttctccaag
4081 agaaggtcat caagactttg gatttttcca caccggggcg cgctgcggct gctgttgctt
4141 ttttgagttt tataaaggat aaatggagcg aagaaaccca tctgagcggg gggtacctgc
4201 tggattttct ggccatgcat ctgtggagag cggttgtgag acacaagaat cgcctgctac
4261 tgttgtcttc cgtccgcccg gcgataatac cgacggagga gcagcagcag cagcaggagg
4321 aagccaggcg gcggcggcag gagcagagcc catggaaccc gagagccggc ctggaccctc
4381 gggaatgact cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg
4441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
4501 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
4561 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
4621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
4681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
4741 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt
4801 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga
4861 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
4921 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
4981 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
5041 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga
5101 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
5161 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
5221 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
5281 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
5341 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
5401 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
5461 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
5521 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
5581 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
5641 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
5701 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
5761 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
5821 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
5881 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
5941 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt
6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
6181 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
6241 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
6301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
6361 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga
6421 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa
6481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
7141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
7741 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca
7801 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
7861 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
7921 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
7981 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa
8041 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
8101 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
8161 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
8221 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
8281 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
8341 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
8401 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
8461 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
8521 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
8581 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
8641 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
8701 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
8761 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
8821 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
8881 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag
8941 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt
9001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
9061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg
9121 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct
9181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
9241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta
9301 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca
9361 tcatcaataa tatacctt
LOCUS dna 9378 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4388
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3432..3437
/dbxref="REBASE:ClaI"
misc_binding 3600..3605
/dbxref="REBASE:XbaI"
misc_binding 3977..3982
/dbxref="REBASE:PstI"
misc_binding 4005..4010
/dbxref="REBASE:EcoRI"
misc_binding 4192..4197
/dbxref="REBASE:KpnI"
misc_binding 4389..4394
/dbxref="REBASE:XhoI"
Promoter 4439..4707
/gene="SV40 prom"
Rep_Origin 4606..4683
/gene="SV40 origin"
ORF 4673..5341
/sequence="ORF_2 rf(2)"
misc_binding 4686..4698
/dbxref="REBASE:SfiI"
Marker 4742..5341
/gene="puro marker"
Other Gene 5951..6003
/gene="delta_U3 other"
Other Gene 6004..6184
/gene="HIV-1_5_LTR other"
Rep_Origin 6690..7309
/gene="pBR322 origin"
Promoter 7962..8011
/gene="NEOKAN prom"
misc_binding 8069..8074
/dbxref="REBASE:BclI"
ORF 8100..8894
/sequence="ORF_4 rf(3)"
Marker 8103..8891
/gene="NTP_II marker"
Rep_Origin 8999..9304
/gene="f1 origin"
BASE COUNT 2322 a 2322 c 2560 g 2174 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcagc caccatgaga catattatct gccacggagg tgttattacc gaagaaatgg
3121 ccgccagtct tttggaccag ctgatcgaag aggtactggc tgataatctt ccacctccta
3181 gccattttga accacctacc cttcacgaac tgtatgattt agacgtgacg gcccccgaag
3241 atcccaacga ggaggcggtt tcgcagattt ttcccgactc tgtaatgttg gcggtgcagg
3301 aagggattga cttactcact tttccgccgg cgcccggttc tccggagccg cctcaccttt
3361 cccggcagcc cgagcagccg gagcagagag ccttgggtcc ggtttctatg ccaaaccttg
3421 taccggaggt gatcgatctt acctgccacg aggctggctt tccacccagt gacgacgagg
3481 atgaagaggg tcctgtgtct gaacctgagc ctgagcccga gccagaaccg gagcctgcaa
3541 gacctacccg ccgtcctaaa atggcgcctg ctatcctgag acgcccgaca tcacctgtgt
3601 ctagagaatg caatagtagt acggatagct gtgactccgg tccttctaac acacctcctg
3661 agatacaccc ggtggtcccg ctgtgcccca ttaaaccagt tgccgtgaga gttggtgggc
3721 gtcgccaggc tgtggaatgt atcgaggact tgcttaacga gcctgggcaa cctttggact
3781 tgagctgtaa acgccccagg ccagagggca gaggaagtct tctaacatgc ggtgacgtgg
3841 aggagaatcc cggccctatg gaggcttggg agtgtttgga agatttttct gctgtgcgta
3901 acttgctgga acagagctct aacagtacct cttggttctg gaggtttctg tggggctcat
3961 cccaggcaaa gttagtctgc agaattaagg aggattacaa gtgggaattc gaagagcttt
4021 tgaagtcctg tggtgagctg tttgattctt tgaatctggg tcaccaggcg cttctccaag
4081 agaaggtcat caagactttg gatttttcca caccggggcg cgctgcggct gctgttgctt
4141 ttttgagttt tataaaggat aaatggagcg aagaaaccca tctgagcggg gggtacctgc
4201 tggattttct ggccatgcat ctgtggagag cggttgtgag acacaagaat cgcctgctac
4261 tgttgtcttc cgtccgcccg gcgataatac cgacggagga gcagcagcag cagcaggagg
4321 aagccaggcg gcggcggcag gagcagagcc catggaaccc gagagccggc ctggaccctc
4381 gggaatgact cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg
4441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
4501 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
4561 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
4621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
4681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
4741 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt
4801 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga
4861 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
4921 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
4981 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
5041 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga
5101 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
5161 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
5221 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
5281 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
5341 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
5401 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
5461 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
5521 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
5581 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
5641 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
5701 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
5761 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
5821 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
5881 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
5941 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt
6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
6181 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
6241 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
6301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
6361 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga
6421 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa
6481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
7141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
7741 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca
7801 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
7861 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
7921 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
7981 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa
8041 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
8101 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
8161 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
8221 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
8281 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
8341 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
8401 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
8461 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
8521 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
8581 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
8641 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
8701 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
8761 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
8821 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
8881 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag
8941 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt
9001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
9061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg
9121 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct
9181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
9241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta
9301 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca
9361 tcatcaataa tatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_3 rf(3)(2901,4388)>>>
|
CMV prom(2885,2966)>>>
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
3061 agctagcaGCCACCATGAGACATATTATCTGCCACGGAGGTGTTATTACCGAAGAAATGG 3120
TCGATCGTCGGTGGTACTCTGTATAATAGACGGTGCCTCCACAATAATGGCTTCTTTACC
3121 CCGCCAGTCTTTTGGACCAGCTGATCGAAGAGGTACTGGCTGATAATCTTCCACCTCCTA 3180
GGCGGTCAGAAAACCTGGTCGACTAGCTTCTCCATGACCGACTATTAGAAGGTGGAGGAT
3181 GCCATTTTGAACCACCTACCCTTCACGAACTGTATGATTTAGACGTGACGGCCCCCGAAG 3240
CGGTAAAACTTGGTGGATGGGAAGTGCTTGACATACTAAATCTGCACTGCCGGGGGCTTC
3241 ATCCCAACGAGGAGGCGGTTTCGCAGATTTTTCCCGACTCTGTAATGTTGGCGGTGCAGG 3300
TAGGGTTGCTCCTCCGCCAAAGCGTCTAAAAAGGGCTGAGACATTACAACCGCCACGTCC
3301 AAGGGATTGACTTACTCACTTTTCCGCCGGCGCCCGGTTCTCCGGAGCCGCCTCACCTTT 3360
TTCCCTAACTGAATGAGTGAAAAGGCGGCCGCGGGCCAAGAGGCCTCGGCGGAGTGGAAA
3361 CCCGGCAGCCCGAGCAGCCGGAGCAGAGAGCCTTGGGTCCGGTTTCTATGCCAAACCTTG 3420
GGGCCGTCGGGCTCGTCGGCCTCGTCTCTCGGAACCCAGGCCAAAGATACGGTTTGGAAC
ClaI
|
3421 TACCGGAGGTGATCGATCTTACCTGCCACGAGGCTGGCTTTCCACCCAGTGACGACGAGG 3480
ATGGCCTCCACTAGCTAGAATGGACGGTGCTCCGACCGAAAGGTGGGTCACTGCTGCTCC
3481 ATGAAGAGGGTCCTGTGTCTGAACCTGAGCCTGAGCCCGAGCCAGAACCGGAGCCTGCAA 3540
TACTTCTCCCAGGACACAGACTTGGACTCGGACTCGGGCTCGGTCTTGGCCTCGGACGTT
3541 GACCTACCCGCCGTCCTAAAATGGCGCCTGCTATCCTGAGACGCCCGACATCACCTGTGT 3600
CTGGATGGGCGGCAGGATTTTACCGCGGACGATAGGACTCTGCGGGCTGTAGTGGACACA
XbaI
|
3601 CTAGAGAATGCAATAGTAGTACGGATAGCTGTGACTCCGGTCCTTCTAACACACCTCCTG 3660
GATCTCTTACGTTATCATCATGCCTATCGACACTGAGGCCAGGAAGATTGTGTGGAGGAC
3661 AGATACACCCGGTGGTCCCGCTGTGCCCCATTAAACCAGTTGCCGTGAGAGTTGGTGGGC 3720
TCTATGTGGGCCACCAGGGCGACACGGGGTAATTTGGTCAACGGCACTCTCAACCACCCG
3721 GTCGCCAGGCTGTGGAATGTATCGAGGACTTGCTTAACGAGCCTGGGCAACCTTTGGACT 3780
CAGCGGTCCGACACCTTACATAGCTCCTGAACGAATTGCTCGGACCCGTTGGAAACCTGA
3781 TGAGCTGTAAACGCCCCAGGCCAGagggcagaggaagtcttctaacatgcggtgacgtgg 3840
ACTCGACATTTGCGGGGTCCGGTCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACC
3841 aggagaatcccggccctatggaggcttgggagtgtttggaagatttttctgctgtgcgta 3900
TCCTCTTAGGGCCGGGATACCTCCGAACCCTCACAAACCTTCTAAAAAGACGACACGCAT
3901 acttgctggaacagagctctaacagtacctcttggttctggaggtttctgtggggctcat 3960
TGAACGACCTTGTCTCGAGATTGTCATGGAGAACCAAGACCTCCAAAGACACCCCGAGTA
PstI EcoRI
| |
3961 cccaggcaaagttagtctgcagaattaaggaggattacaagtgggaattcgaagagcttt 4020
GGGTCCGTTTCAATCAGACGTCTTAATTCCTCCTAATGTTCACCCTTAAGCTTCTCGAAA
4021 tgaagtcctgtggtgagctgtttgattctttgaatctgggtcaccaggcgcttctccaag 4080
ACTTCAGGACACCACTCGACAAACTAAGAAACTTAGACCCAGTGGTCCGCGAAGAGGTTC
4081 agaaggtcatcaagactttggatttttccacaccggggcgcgctgcggctgctgttgctt 4140
TCTTCCAGTAGTTCTGAAACCTAAAAAGGTGTGGCCCCGCGCGACGCCGACGACAACGAA
KpnI
|
4141 ttttgagttttataaaggataaatggagcgaagaaacccatctgagcggggggtacctgc 4200
AAAACTCAAAATATTTCCTATTTACCTCGCTTCTTTGGGTAGACTCGCCCCCCATGGACG
4201 tggattttctggccatgcatctgtggagagcggttgtgagacacaagaatcgcctgctac 4260
ACCTAAAAGACCGGTACGTAGACACCTCTCGCCAACACTCTGTGTTCTTAGCGGACGATG
4261 tgttgtcttccgtccgcccggcgataataccgacggaggagcagcagcagcagcaggagg 4320
ACAACAGAAGGCAGGCGGGCCGCTATTATGGCTGCCTCCTCGTCGTCGTCGTCGTCCTCC
4321 aagccaggcggcggcggcaggagcagagcccatggaacccgagagccggcctggaccctc 4380
TTCGGTCCGCCGCCGCCGTCCTCGTCTCGGGTACCTTGGGCTCTCGGCCGGACCTGGGAG
XhoI SV40 prom(4439,4707)>>>
| |
4381 gggaatgactcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtgg 4440
CCCTTACTGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACC
4441 tgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattag 4500
ACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATC
4501 tcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatg 4560
AGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTAC
SV40 origin(4606,4683)>>>
|
4561 catctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaact 4620
GTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGA
ORF_2 rf(2)(4673,5341)>>>
|
4621 ccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagag 4680
GGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTC
SfiI
|
4681 gccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggc 4740
CGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCG
puro marker(4742,5341)>>>
|
4741 catgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgt 4800
GTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCA
4801 acgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccgga 4860
TGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCT
4861 ccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcga 4920
GGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCT
4921 catcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccgga 4980
GTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCT
4981 gagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcgg 5040
CTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCC
5041 ttcccggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaagga 5100
AAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCT
5101 gcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctggg 5160
CGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCC
5161 cagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcct 5220
GTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGA
5221 ggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgc 5280
CCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCG
5281 cgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctg 5340
GCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGAC
5341 aacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattct 5400
TTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGA
5401 taactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgc 5460
ATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACG
5461 tattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctct 5520
ATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGA
5521 ttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctga 5580
AATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACT
5581 cgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgc 5640
GCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCG
5641 tttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggac 5700
AAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTG
5701 aggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctt 5760
TCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAA
5761 tccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgt 5820
AGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCA
5821 cccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcc 5880
GGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGG
5881 tcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccg 5940
AGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGC
delta_U3 other(5951,6003)>>>
|
5941 cctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgt 6000
GGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACA
HIV-1_5_LTR other(6004,6184)>>>
|
6001 actgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaac 6060
TGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTG
6061 ccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctg 6120
GGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGAC
6121 ttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctct 6180
AACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGA
6181 agcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaata 6240
TCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTAT
6241 tcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagc 6300
AGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCG
6301 atcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaa 6360
TAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTT
6361 ctcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatga 6420
GAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACT
6421 aatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaa 6480
TTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTT
6481 ataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctc 6540
TATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAG
6541 actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg 6600
TGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGC
6601 gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc 6660
CATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCG
pBR322 origin(6690,7309)<<<
|
6661 cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc 6720
GTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCG
6721 ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga 6780
GGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCT
6781 ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc 6840
GATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGG
6841 ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcat 6900
GACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTA
6901 agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg 6960
TCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACAC
6961 cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc 7020
GTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGG
7021 aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga 7080
TTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCT
7081 gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact 7140
CGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGA
7141 agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt 7200
TCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAA
7201 ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag 7260
CCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTC
7261 cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg 7320
GTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCC
7321 tctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa 7380
AGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTT
7381 aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata 7440
TCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATAT
7441 tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcg 7500
ATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGC
7501 atctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgata 7560
TAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTAT
7561 cgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccg 7620
GCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGC
7621 gctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcct 7680
CGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGA
7681 gcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagt 7740
CGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCA
7741 tcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttca 7800
AGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGT
7801 cgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggcta 7860
GCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGAT
7861 tctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacat 7920
AGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTA
NEOKAN prom(7962,8011)>>>
|
7921 ggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctgggg 7980
CCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCC
7981 cgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaa 8040
GCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTT
BclI
|
8041 ggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgca 8100
CCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGT
NTP_II marker(8103,8891)>>>
|
ORF_4 rf(3)(8100,8894)>>>
| |
8101 tgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcg 8160
ACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGC
8161 gctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcag 8220
CGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTC
8221 cgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgc 8280
GCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACG
8281 aagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgc 8340
TTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACG
8341 tcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcagg 8400
AGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCC
8401 atctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgc 8460
TAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACG
8461 ggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgca 8520
CCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGT
8521 tcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaag 8580
AGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTC
8581 agcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacg 8640
TCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGC
8641 gcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatg 8700
CGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTAC
8701 gccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggaca 8760
CGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGT
8761 tagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcc 8820
ATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGG
8821 tcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttg 8880
AGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAAC
8881 acgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaatag 8940
TGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATC
f1 origin(8999,9304)<<<
|
8941 gccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgtt 9000
CGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAA
9001 gttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcga 9060
CAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCT
9061 aaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttg 9120
TTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAAC
9121 cggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagct 9180
GCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGA
9181 tgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggc 9240
ACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCG
9241 gctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgctta 9300
CGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAAT
9301 atgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaaca 9360
TACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGT
9361 tcatcaataatatacctt 9378
AGTAGTTATTATATGGAA
LOCUS dna 9378 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4388
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3432..3437
/dbxref="REBASE:ClaI"
misc_binding 3600..3605
/dbxref="REBASE:XbaI"
misc_binding 3977..3982
/dbxref="REBASE:PstI"
misc_binding 4005..4010
/dbxref="REBASE:EcoRI"
misc_binding 4192..4197
/dbxref="REBASE:KpnI"
misc_binding 4389..4394
/dbxref="REBASE:XhoI"
Promoter 4439..4707
/gene="SV40 prom"
Rep_Origin 4606..4683
/gene="SV40 origin"
ORF 4673..5341
/sequence="ORF_2 rf(2)"
misc_binding 4686..4698
/dbxref="REBASE:SfiI"
Marker 4742..5341
/gene="puro marker"
Other Gene 5951..6003
/gene="delta_U3 other"
Other Gene 6004..6184
/gene="HIV-1_5_LTR other"
Rep_Origin 6690..7309
/gene="pBR322 origin"
Promoter 7962..8011
/gene="NEOKAN prom"
misc_binding 8069..8074
/dbxref="REBASE:BclI"
ORF 8100..8894
/sequence="ORF_4 rf(3)"
Marker 8103..8891
/gene="NTP_II marker"
Rep_Origin 8999..9304
/gene="f1 origin"
BASE COUNT 2322 a 2322 c 2560 g 2174 t 0 others