pLenti-EF1a-Blank
| Vector Name | pLenti-EF1a-Blank |
| VectorType | Lentiviral Blank Control Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc
1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg
2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
3001 tttcaggtgt cgtgaggaat ttcgactgct agactgcagc tcgagtaccc atacgacgtc
3061 ccagactacg cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg
3121 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
3181 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
3241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
3301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
3361 ccagaagtag tgaggaggct tttttggagg ccatgaccga gtacaagccc acggtgcgcc
3421 tcgccacccg cgacgacgtc cctcgggccg tacgcaccct cgccgccgcg ttcgccgact
3481 accccgccac gcgccacacc gtggacccgg accgccacat cgagcgggtc accgagctgc
3541 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
3601 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
3661 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
3721 aagggctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
3781 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
3841 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
3901 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
3961 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt
4021 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
4081 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
4141 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
4201 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
4261 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
4321 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
4381 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
4441 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
4501 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac
4561 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc
4621 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
4681 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
4741 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
4801 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
4861 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
4921 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
4981 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca
5041 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
5101 ttaatgaatc ggccaacgga tgtcggtgtg aaataccgca cagatgcgta aggagaaaat
5161 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
5221 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
5281 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
5341 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
5401 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
5461 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
5521 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5581 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5641 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5701 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5761 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
5821 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5881 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
5941 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
6001 gttaagggat tttggccatg agattatcaa aaaggatctt cacctagatc cttttcacgt
6061 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
6121 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
6181 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
6241 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
6301 tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga
6361 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
6421 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
6481 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
6541 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
6601 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
6661 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
6721 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
6781 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
6841 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
6901 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
6961 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
7021 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
7081 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
7141 agttcttctg aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc
7201 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
7261 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
7321 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
7381 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
7441 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
7501 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
7561 gcgccgctac agggcgcgtc cattcgccat tcaggatcga attaattctt aattaacatc
7621 atcaataata tacctttttt ggattgaagc caatatgata atgagggggt ggagtttgtg
7681 acgtggcgcg gggcgtggga acggggcggg tgacgtagta gtgtggcgga agtgtgatgt
7741 tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca aaagtgacgt ttttggtgtg
7801 cgccggtgta cacaggaagt gacaattttc gcgcggtttt aggcggatgt tgtagtaaat
7861 ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa aactgaataa gaggaagtga
7921 aatctgaata attttgtgtt actcatagcg cgtaatactc tcatgagcgg atacatattt
7981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
8041 cctgacgtaa ctataacggt cctaaggtag cgaaaatgta gtcttatgca atactcttgt
8101 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
8161 gtgcatgccg attggtggaa gtaaggtggt acgat
//
LOCUS dna 8195 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
ORF 1775..2755
/sequence="ORF_4 rf(5)"
Promoter 1838..3015
/gene="EF1a prom"
misc_binding 2450..2455
/dbxref="REBASE:ApaI"
Promoter 3090..3358
/gene="SV40 prom"
Rep_Origin 3257..3334
/gene="SV40 origin"
ORF 3324..3992
/sequence="ORF_2 rf(3)"
misc_binding 3337..3349
/dbxref="REBASE:SfiI"
Marker 3393..3992
/gene="puro marker"
Other Gene 4602..4654
/gene="delta_U3 other"
Other Gene 4655..4835
/gene="HIV-1_5_LTR other"
Rep_Origin 5342..5961
/gene="pBR322 origin"
Promoter 6219..6268
/gene="NEOKAN prom"
misc_binding 6326..6331
/dbxref="REBASE:BclI"
ORF 6357..7151
/sequence="ORF_3 rf(3)"
Marker 6360..7148
/gene="NTP_II marker"
Rep_Origin 7256..7562
/gene="f1 origin"
Other Gene 7617..7718
/gene="RITR other"
Other Gene 7617..7718
/gene="LITR other"
Other Gene 7798..7946
/gene="Encap other"
BASE COUNT 1953 a 2021 c 2335 g 1886 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc
1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg
2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
3001 tttcaggtgt cgtgaggaat ttcgactgct agactgcagc tcgagtaccc atacgacgtc
3061 ccagactacg cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg
3121 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
3181 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
3241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
3301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
3361 ccagaagtag tgaggaggct tttttggagg ccatgaccga gtacaagccc acggtgcgcc
3421 tcgccacccg cgacgacgtc cctcgggccg tacgcaccct cgccgccgcg ttcgccgact
3481 accccgccac gcgccacacc gtggacccgg accgccacat cgagcgggtc accgagctgc
3541 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
3601 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
3661 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
3721 aagggctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
3781 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
3841 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
3901 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
3961 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt
4021 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
4081 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
4141 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
4201 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
4261 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
4321 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
4381 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
4441 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
4501 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac
4561 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc
4621 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
4681 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
4741 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
4801 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
4861 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
4921 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
4981 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca
5041 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
5101 ttaatgaatc ggccaacgga tgtcggtgtg aaataccgca cagatgcgta aggagaaaat
5161 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
5221 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
5281 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
5341 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
5401 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
5461 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
5521 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5581 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5641 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5701 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5761 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
5821 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5881 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
5941 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
6001 gttaagggat tttggccatg agattatcaa aaaggatctt cacctagatc cttttcacgt
6061 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
6121 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
6181 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
6241 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
6301 tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga
6361 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
6421 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
6481 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
6541 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
6601 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
6661 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
6721 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
6781 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
6841 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
6901 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
6961 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
7021 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
7081 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
7141 agttcttctg aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc
7201 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
7261 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
7321 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
7381 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
7441 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
7501 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
7561 gcgccgctac agggcgcgtc cattcgccat tcaggatcga attaattctt aattaacatc
7621 atcaataata tacctttttt ggattgaagc caatatgata atgagggggt ggagtttgtg
7681 acgtggcgcg gggcgtggga acggggcggg tgacgtagta gtgtggcgga agtgtgatgt
7741 tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca aaagtgacgt ttttggtgtg
7801 cgccggtgta cacaggaagt gacaattttc gcgcggtttt aggcggatgt tgtagtaaat
7861 ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa aactgaataa gaggaagtga
7921 aatctgaata attttgtgtt actcatagcg cgtaatactc tcatgagcgg atacatattt
7981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
8041 cctgacgtaa ctataacggt cctaaggtag cgaaaatgta gtcttatgca atactcttgt
8101 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
8161 gtgcatgccg attggtggaa gtaaggtggt acgat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
ORF_4 rf(5)(1775,2755)<<<
|
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
EF1a prom(1838,3015)>>>
|
1801 aaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtcagtgggc 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGTCACCCG
1861 agagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccgg 1920
TCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCC
1921 tgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcct 1980
ACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGA
1981 ttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttt 2040
AAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAA
2041 tcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctgg 2100
AGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACC
2101 cctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcagtacgtg 2160
GGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCAC
2161 attcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa 2220
TAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATT
2221 ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtg 2280
CCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCAC
2281 cgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaa 2340
GCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATT
2341 aatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggc 2400
TTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCG
ApaI
|
2401 caagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcg 2460
GTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGC
2461 tcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgg 2520
AGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCC
2521 gggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgcc 2580
CCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGG
2581 ccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg 2640
GGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGC
2641 cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcg 2700
GAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGC
2701 ggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgac 2760
CCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTG
2761 tccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacg 2820
AGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGC
2821 tcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtg 2880
AGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCAC
2881 gagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcccttttt 2940
CTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAA
2941 gagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttcca 3000
CTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGT
3001 tttcaggtgtcgtgaggaatttcgactgctagactgcagctcgagtacccatacgacgtc 3060
AAAGTCCACAGCACTCCTTAAAGCTGACGATCTGACGTCGAGCTCATGGGTATGCTGCAG
SV40 prom(3090,3358)>>>
|
3061 ccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcagg 3120
GGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCC
3121 cagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccagg 3180
GTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCC
3181 ctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtccc 3240
GAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGG
SV40 origin(3257,3334)>>>
|
3241 gcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccca 3300
CGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGT
SfiI
|
ORF_2 rf(3)(3324,3992)>>>
| |
3301 tggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctatt 3360
ACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAA
puro marker(3393,3992)>>>
|
3361 ccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcc 3420
GGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGG
3421 tcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgact 3480
AGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGA
3481 accccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgc 3540
TGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACG
3541 aagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacg 3600
TTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGC
3601 gcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccg 3660
CGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGC
3661 agatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatgg 3720
TCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACC
3721 aagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcg 3780
TTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGC
3781 tctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggagg 3840
AGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCC
3841 cggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcccct 3900
GCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGA
3901 tctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgca 3960
AGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGT
3961 cctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggatt 4020
GGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAA
4021 acaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtg 4080
TGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACAC
4081 gatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttct 4140
CTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGA
4141 cctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggc 4200
GGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCG
4201 aacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgcca 4260
TTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGT
4261 ccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaac 4320
GGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTG
4321 tcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaatt 4380
AGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAA
4381 ccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacct 4440
GGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGA
4441 ggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttc 4500
CCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAG
4501 cttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagac 4560
GAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTG
delta_U3 other(4602,4654)>>>
|
4561 gagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcactc 4620
CTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAG
HIV-1_5_LTR other(4655,4835)>>>
|
4621 ccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctg 4680
GGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGAC
4681 agcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcc 4740
TCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGG
4741 ttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct 4800
AACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGA
4801 cagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattat 4860
GTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATA
4861 tcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgc 4920
AGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACG
4921 agcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttt 4980
TCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAA
4981 ttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggca 5040
AAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGT
5041 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 5100
AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT
5101 ttaatgaatcggccaacggatgtcggtgtgaaataccgcacagatgcgtaaggagaaaat 5160
AATTACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTA
5161 accgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggc 5220
TGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCG
5221 tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg 5280
ACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCC
5281 ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg 5340
TATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCC
pBR322 origin(5342,5961)<<<
|
5341 ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac 5400
GGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTG
5401 gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg 5460
CGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGAC
5461 gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct 5520
CTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGA
5521 ttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcgg 5580
AAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCC
5581 tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct 5640
ACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGA
5641 gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac 5700
CGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTG
5701 tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt 5760
ACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCA
5761 tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc 5820
AGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAG
5821 tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca 5880
ACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGT
5881 ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat 5940
GGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTA
5941 ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac 6000
GAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTG
6001 gttaagggattttggccatgagattatcaaaaaggatcttcacctagatccttttcacgt 6060
CAATTCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCA
6061 agaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatct 6120
TCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGA
6121 ggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggc 6180
CCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCG
NEOKAN prom(6219,6268)>>>
|
6181 gatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgc 6240
CTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCG
6241 cctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaagga 6300
GGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCCT
BclI ORF_3 rf(3)(6357,7151)>>>
| |
6301 tctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatga 6360
AGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACT
NTP_II marker(6360,7148)>>>
|
6361 ttgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggct 6420
AACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGA
6421 atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgc 6480
TACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCG
6481 aggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaag 6540
TCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTC
6541 acgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcg 6600
TGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGC
6601 acgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatc 6660
TGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAG
6661 tcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggc 6720
AGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCG
6721 ggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcg 6780
CCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGC
6781 agcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagc 6840
TCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCG
6841 atcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcg 6900
TAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGC
6901 aggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggcc 6960
TCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGG
6961 gcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatag 7020
CGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATC
7021 cgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcg 7080
GCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGC
7081 tgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacg 7140
ACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGC
7141 agttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggcc 7200
TCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGG
f1 origin(7256,7562)<<<
|
7201 gaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgtt 7260
CTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAA
7261 ccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaa 7320
GGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTT
7321 accgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttgggg 7380
TGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCC
7381 tcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttga 7440
AGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACT
7441 cggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgct 7500
GCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGA
7501 agggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaat 7560
TCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTA
LITR other(7617,7718)>>>
|
RITR other(7617,7718)<<<
|
7561 gcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatc 7620
CGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAG
7621 atcaataatataccttttttggattgaagccaatatgataatgagggggtggagtttgtg 7680
TAGTTATTATATGGAAAAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACAC
7681 acgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgt 7740
TGCACCGCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACA
Encap other(7798,7946)>>>
|
7741 tgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtg 7800
ACGTTCACACCGCCTTGTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACAC
7801 cgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaat 7860
GCGGCCACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTA
7861 ttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtga 7920
AACCCGCATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACT
7921 aatctgaataattttgtgttactcatagcgcgtaatactctcatgagcggatacatattt 7980
TTAGACTTATTAAAACACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAA
7981 gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgcca 8040
CTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGT
8041 cctgacgtaactataacggtcctaaggtagcgaaaatgtagtcttatgcaatactcttgt 8100
GGACTGCATTGATATTGCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAACA
8101 agtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcacc 8160
TCAGAACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGG
8161 gtgcatgccgattggtggaagtaaggtggtacgat 8195
CACGTACGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 8195 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
ORF 1775..2755
/sequence="ORF_4 rf(5)"
Promoter 1838..3015
/gene="EF1a prom"
misc_binding 2450..2455
/dbxref="REBASE:ApaI"
Promoter 3090..3358
/gene="SV40 prom"
Rep_Origin 3257..3334
/gene="SV40 origin"
ORF 3324..3992
/sequence="ORF_2 rf(3)"
misc_binding 3337..3349
/dbxref="REBASE:SfiI"
Marker 3393..3992
/gene="puro marker"
Other Gene 4602..4654
/gene="delta_U3 other"
Other Gene 4655..4835
/gene="HIV-1_5_LTR other"
Rep_Origin 5342..5961
/gene="pBR322 origin"
Promoter 6219..6268
/gene="NEOKAN prom"
misc_binding 6326..6331
/dbxref="REBASE:BclI"
ORF 6357..7151
/sequence="ORF_3 rf(3)"
Marker 6360..7148
/gene="NTP_II marker"
Rep_Origin 7256..7562
/gene="f1 origin"
Other Gene 7617..7718
/gene="RITR other"
Other Gene 7617..7718
/gene="LITR other"
Other Gene 7798..7946
/gene="Encap other"
BASE COUNT 1953 a 2021 c 2335 g 1886 t 0 others