pLenti-EF1a-dCas9-KRAB
| Vector Name | pLenti-EF1a-dCas9-KRAB |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagaa ataattttgt ttaactttaa
3661 gaaggagata taccatggac aagaagtact ccattgggct cgcgatcggc acaaacagcg
3721 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
3781 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
3841 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
3901 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
3961 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
4021 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
4081 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
4141 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
4201 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
4261 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
4321 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
4381 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
4441 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
4501 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
4561 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
4621 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
4681 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
4741 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
4801 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
4861 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
4921 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
4981 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
5041 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
5101 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
5161 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
5221 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
5281 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
5341 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
5401 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
5461 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
5521 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
5581 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
5641 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
5701 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
5761 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
5821 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
5881 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
5941 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
6001 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
6061 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
6121 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
6181 acgacgtgga tgcgatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
6241 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
6301 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
6361 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
6421 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
6481 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
6541 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
6601 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
6661 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
6721 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
6781 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
6841 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
6901 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
6961 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
7021 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
7081 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
7141 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
7201 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
7261 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
7321 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
7381 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
7441 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
7501 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
7561 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
7621 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
7681 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
7741 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
7801 agaggaaggt ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
7861 cggccgcccg gacactggtg accttcaagg atgtatttgt ggacttcacc agggaggagt
7921 ggaagctgct ggacactgct cagcagatcg tgtacagaaa tgtgatgctg gagaactata
7981 agaacctggt ttccttgggt tatcagctta ctaagccaga tgtgatcctc cggttggaga
8041 agggagaaga gcccactagt ccaaaaaaga aaagaaaggt gtgagtttaa acacgcgtgg
8101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
8161 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
8221 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
8281 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
8341 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
8401 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt
8461 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga
8521 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
8581 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
8641 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
8701 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga
8761 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
8821 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
8881 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
8941 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
9001 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
9061 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
9121 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
9181 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
9241 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
9301 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
9361 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
9421 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
9481 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
9541 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
9601 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt
9661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
9721 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
9781 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
9841 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
9901 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
9961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
10021 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga
10081 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa
10141 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
10201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
10261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
10321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
10381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
10441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
10501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
10561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
10621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
10681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
10741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
10801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
10861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
10921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
10981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
11041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
11101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
11161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
11221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
11281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
11341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
11401 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca
11461 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
11521 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
11581 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
11641 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa
11701 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
11761 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
11821 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
11881 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
11941 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
12001 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
12061 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
12121 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
12181 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
12241 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
12301 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
12361 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
12421 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
12481 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
12541 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag
12601 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt
12661 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
12721 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg
12781 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct
12841 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
12901 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta
12961 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca
13021 tcatcaataa tatacctt
LOCUS dna 13038 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(6)"
Promoter 2444..3621
/gene="EF1a prom"
misc_binding 3056..3061
/dbxref="REBASE:ApaI"
ORF 3675..8084
/sequence="ORF_3 rf(3)"
misc_binding 4152..4157
/dbxref="REBASE:NdeI"
misc_binding 5041..5046
/dbxref="REBASE:SmaI"
misc_binding 5041..5046
/dbxref="REBASE:XmaI"
misc_binding 5680..5685
/dbxref="REBASE:BamHI"
misc_binding 7497..7502
/dbxref="REBASE:EcoRI"
misc_binding 7860..7867
/dbxref="REBASE:NotI"
Promoter 8099..8367
/gene="SV40 prom"
Rep_Origin 8266..8343
/gene="SV40 origin"
ORF 8333..9001
/sequence="ORF_2 rf(2)"
misc_binding 8346..8358
/dbxref="REBASE:SfiI"
Marker 8402..9001
/gene="puro marker"
Other Gene 9611..9663
/gene="delta_U3 other"
Other Gene 9664..9844
/gene="HIV-1_5_LTR other"
Rep_Origin 10350..10969
/gene="pBR322 origin"
Promoter 11622..11671
/gene="NEOKAN prom"
ORF 11760..12554
/sequence="ORF_4 rf(3)"
Marker 11763..12551
/gene="NTP_II marker"
Rep_Origin 12659..12964
/gene="f1 origin"
BASE COUNT 3441 a 3147 c 3521 g 2929 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagaa ataattttgt ttaactttaa
3661 gaaggagata taccatggac aagaagtact ccattgggct cgcgatcggc acaaacagcg
3721 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
3781 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
3841 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
3901 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
3961 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
4021 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
4081 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
4141 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
4201 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
4261 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
4321 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
4381 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
4441 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
4501 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
4561 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
4621 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
4681 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
4741 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
4801 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
4861 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
4921 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
4981 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
5041 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
5101 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
5161 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
5221 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
5281 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
5341 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
5401 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
5461 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
5521 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
5581 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
5641 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
5701 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
5761 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
5821 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
5881 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
5941 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
6001 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
6061 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
6121 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
6181 acgacgtgga tgcgatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
6241 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
6301 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
6361 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
6421 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
6481 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
6541 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
6601 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
6661 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
6721 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
6781 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
6841 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
6901 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
6961 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
7021 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
7081 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
7141 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
7201 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
7261 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
7321 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
7381 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
7441 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
7501 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
7561 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
7621 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
7681 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
7741 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
7801 agaggaaggt ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
7861 cggccgcccg gacactggtg accttcaagg atgtatttgt ggacttcacc agggaggagt
7921 ggaagctgct ggacactgct cagcagatcg tgtacagaaa tgtgatgctg gagaactata
7981 agaacctggt ttccttgggt tatcagctta ctaagccaga tgtgatcctc cggttggaga
8041 agggagaaga gcccactagt ccaaaaaaga aaagaaaggt gtgagtttaa acacgcgtgg
8101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
8161 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
8221 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
8281 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
8341 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
8401 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt
8461 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga
8521 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
8581 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
8641 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
8701 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga
8761 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
8821 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
8881 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
8941 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
9001 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
9061 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
9121 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
9181 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
9241 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
9301 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
9361 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
9421 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
9481 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
9541 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
9601 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt
9661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
9721 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
9781 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
9841 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
9901 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
9961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
10021 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga
10081 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa
10141 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
10201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
10261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
10321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
10381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
10441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
10501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
10561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
10621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
10681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
10741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
10801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
10861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
10921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
10981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
11041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
11101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
11161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
11221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
11281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
11341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
11401 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca
11461 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
11521 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
11581 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
11641 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa
11701 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
11761 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
11821 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
11881 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
11941 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
12001 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
12061 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
12121 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
12181 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
12241 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
12301 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
12361 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
12421 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
12481 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
12541 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag
12601 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt
12661 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
12721 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg
12781 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct
12841 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
12901 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta
12961 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca
13021 tcatcaataa tatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
ORF_5 rf(6)(2381,3361)<<<
|
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
EF1a prom(2444,3621)>>>
|
2401 aattcaaaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtca 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGT
2461 gtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattg 2520
CACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAC
2521 aaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggct 2580
TTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGA
2581 ccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgt 2640
GGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCA
2641 tctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgg 2700
AGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCC
2701 gcctggcctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcag 2760
CGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTC
2761 tacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgc 2820
ATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACG
2821 gcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgc 2880
CGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCG
2881 cgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagcc 2940
GCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGG
2941 atttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaat 3000
TAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTA
3001 gcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcc 3060
CGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGG
ApaI
|
3061 cgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatc 3120
GCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAG
3121 ggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgt 3180
CCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACA
3181 atcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaaga 3240
TAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCT
3241 tggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagag 3300
ACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTC
3301 cgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttca 3360
GCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGT
3361 tgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttgg 3420
ACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACC
3421 agtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgag 3480
TCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTC
3481 tgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcc 3540
ACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGG
3541 ctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttttttt 3600
GAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAA
3601 cttccatttcaggtgtcgtgaggaatttcgactgctagaaataattttgtttaactttaa 3660
GAAGGTAAAGTCCACAGCACTCCTTAAAGCTGACGATCTTTATTAAAACAAATTGAAATT
ORF_3 rf(3)(3675,8084)>>>
|
3661 gaaggagatataccatggacaagaagtactccattgggctcgcgatcggcacaaacagcg 3720
CTTCCTCTATATGGTACCTGTTCTTCATGAGGTAACCCGAGCGCTAGCCGTGTTTGTCGC
3721 tcggctgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgg 3780
AGCCGACCCGGCAGTAATGCCTGCTCATGTTCCACGGCTCGTTTTTTAAGTTTCAAGACC
3781 gcaataccgatcgccacagcataaagaagaacctcattggcgccctcctgttcgactccg 3840
CGTTATGGCTAGCGGTGTCGTATTTCTTCTTGGAGTAACCGCGGGAGGACAAGCTGAGGC
3841 gggagacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaa 3900
CCCTCTGCCGGCTTCGGTGCGCCGAGTTTTCTTGTCGTGCCGCGTCTATATGGGCGTCTT
3901 agaatcggatctgctacctgcaggagatctttagtaatgagatggctaaggtggatgact 3960
TCTTAGCCTAGACGATGGACGTCCTCTAGAAATCATTACTCTACCGATTCCACCTACTGA
3961 ctttcttccataggctggaggagtcctttttggtggaggaggataaaaagcacgagcgcc 4020
GAAAGAAGGTATCCGACCTCCTCAGGAAAAACCACCTCCTCCTATTTTTCGTGCTCGCGG
4021 acccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatat 4080
TGGGTTAGAAACCGTTATAGCACCTGCTCCACCGCATGGTACTTTTCATGGGTTGGTATA
4081 atcatctgaggaagaagcttgtagacagtactgataaggctgacttgcggttgatctatc 4140
TAGTAGACTCCTTCTTCGAACATCTGTCATGACTATTCCGACTGAACGCCAACTAGATAG
NdeI
|
4141 tcgcgctggcgcatatgatcaaatttcggggacacttcctcatcgagggggacctgaacc 4200
AGCGCGACCGCGTATACTAGTTTAAAGCCCCTGTGAAGGAGTAGCTCCCCCTGGACTTGG
4201 cagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttt 4260
GTCTGTTGTCGCTACAGCTGTTTGAGAAATAGGTTGACCAAGTCTGAATGTTAGTCGAAA
4261 tcgaagagaacccgatcaacgcatccggagttgacgccaaagcaatcctgagcgctaggc 4320
AGCTTCTCTTGGGCTAGTTGCGTAGGCCTCAACTGCGGTTTCGTTAGGACTCGCGATCCG
4321 tgtccaaatcccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacg 4380
ACAGGTTTAGGGCCGCCGAGCTTTTGGAGTAGCGTGTCGAGGGACCCCTCTTCTTCTTGC
4381 gcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaact 4440
CGGACAAACCATTAGAATAGCGGGACAGTGAGCCCGACTGGGGGTTGAAATTTAGATTGA
4441 tcgacctggccgaagatgccaagcttcaactgagcaaagacacctacgatgatgatctcg 4500
AGCTGGACCGGCTTCTACGGTTCGAAGTTGACTCGTTTCTGTGGATGCTACTACTAGAGC
4501 acaatctgctggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacc 4560
TGTTAGACGACCGGGTCTAGCCGCTGGTCATGCGTCTGGAAAAAAACCGCCGTTTCTTGG
4561 tgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctc 4620
ACAGTCTGCGGTAAGACGACTCACTATAAGACGCTCACTTGTGCCTCTAGTGGTTTCGAG
4621 cgctgagcgctagtatgatcaagcgctatgatgagcaccaccaagacttgactttgctga 4680
GCGACTCGCGATCATACTAGTTCGCGATACTACTCGTGGTGGTTCTGAACTGAAACGACT
4681 aggcccttgtcagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtcta 4740
TCCGGGAACAGTCTGTCGTTGACGGACTCTTCATGTTCCTTTAAAAGAAGCTAGTCAGAT
4741 aaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaattta 4800
TTTTACCGATGCGGCCTATGTAACTGCCGCCTCGTTCGGTCCTCCTTAAAATGTTTAAAT
4801 ttaagcccatcttggaaaaaatggacggcaccgaggagctgctggtaaagcttaacagag 4860
AATTCGGGTAGAACCTTTTTTACCTGCCGTGGCTCCTCGACGACCATTTCGAATTGTCTC
4861 aagatctgttgcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacc 4920
TTCTAGACAACGCGTTTGTCGCGTGAAAGCTGTTACCTTCGTAGGGGGTGGTCTAAGTGG
4921 tgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagata 4980
ACCCGCTTGACGTGCGATAGGAGTCCGCCGTTCTCCTAAAGATGGGGAAAAACTTTCTAT
4981 acagggaaaagattgagaaaatcctcacatttcggataccctactatgtaggccccctcg 5040
TGTCCCTTTTCTAACTCTTTTAGGAGTGTAAAGCCTATGGGATGATACATCCGGGGGAGC
SmaI
|
XmaI
| |
5041 cccggggaaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccct 5100
GGGCCCCTTTAAGGTCTAAGCGCACCTACTGAGCGTTTAGTCTTCTCTGGTAGTGAGGGA
5101 ggaacttcgaggaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatga 5160
CCTTGAAGCTCCTTCAGCACCTATTCCCCCGGAGACGGGTCAGGAAGTAGCTTTCCTACT
5161 ctaactttgataaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacg 5220
GATTGAAACTATTTTTAGACGGATTGCTTTTCCACGAAGGATTTGTGAGAGACGACATGC
5221 agtacttcacagtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaa 5280
TCATGAAGTGTCAAATATTGCTCGAGTGGTTCCAGTTTATGCAGTGTCTTCCCTACTCTT
5281 agccagcattcctgtctggagagcagaagaaagctatcgtggacctcctcttcaagacga 5340
TCGGTCGTAAGGACAGACCTCTCGTCTTCTTTCGATAGCACCTGGAGGAGAAGTTCTGCT
5341 accggaaagttaccgtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcg 5400
TGGCCTTTCAATGGCACTTTGTCGAGTTTCTTCTGATAAAGTTTTTCTAACTTACAAAGC
5401 actctgttgaaatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacg 5460
TGAGACAACTTTAGTCGCCTCACCTCCTAGCGAAGTTGCGTAGGGACCCTTGCATAGTGC
5461 atctcctgaaaatcattaaagacaaggacttcctggacaatgaggagaacgaggacattc 5520
TAGAGGACTTTTAGTAATTTCTGTTCCTGAAGGACCTGTTACTCCTCTTGCTCCTGTAAG
5521 ttgaggacattgtcctcacccttacgttgtttgaagatagggagatgattgaagaacgct 5580
AACTCCTGTAACAGGAGTGGGAATGCAACAAACTTCTATCCCTCTACTAACTTCTTGCGA
5581 tgaaaacttacgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgat 5640
ACTTTTGAATGCGAGTAGAGAAGCTGCTGTTTCAGTACTTTGTCGAGTTCTCCGCGGCTA
BamHI
|
5641 atacaggatgggggcggctgtcaagaaaactgatcaatgggatccgagacaagcagagtg 5700
TATGTCCTACCCCCGCCGACAGTTCTTTTGACTAGTTACCCTAGGCTCTGTTCGTCTCAC
5701 gaaagacaatcctggattttcttaagtccgatggatttgccaaccggaacttcatgcagt 5760
CTTTCTGTTAGGACCTAAAAGAATTCAGGCTACCTAAACGGTTGGCCTTGAAGTACGTCA
5761 tgatccatgatgactctctcacctttaaggaggacatccagaaagcacaagtttctggcc 5820
ACTAGGTACTACTGAGAGAGTGGAAATTCCTCCTGTAGGTCTTTCGTGTTCAAAGACCGG
5821 agggggacagtcttcacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagg 5880
TCCCCCTGTCAGAAGTGCTCGTGTAGCGATTAGAACGTCCATCGGGTCGATAGTTTTTCC
5881 gaatactgcagaccgttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagc 5940
CTTATGACGTCTGGCAATTCCAGCACCTACTTGAGCAGTTTCATTACCCTTCCGTATTCG
5941 ccgagaatatcgttatcgagatggcccgagagaaccaaactacccagaagggacagaaga 6000
GGCTCTTATAGCAATAGCTCTACCGGGCTCTCTTGGTTTGATGGGTCTTCCCTGTCTTCT
6001 acagtagggaaaggatgaagaggattgaagagggtataaaagaactggggtcccaaatcc 6060
TGTCATCCCTTTCCTACTTCTCCTAACTTCTCCCATATTTTCTTGACCCCAGGGTTTAGG
6061 ttaaggaacacccagttgaaaacacccagcttcagaatgagaagctctacctgtactacc 6120
AATTCCTTGTGGGTCAACTTTTGTGGGTCGAAGTCTTACTCTTCGAGATGGACATGATGG
6121 tgcagaacggcagggacatgtacgtggatcaggaactggacatcaatcggctctccgact 6180
ACGTCTTGCCGTCCCTGTACATGCACCTAGTCCTTGACCTGTAGTTAGCCGAGAGGCTGA
6181 acgacgtggatgcgatcgtgccccagtcttttctcaaagatgattctattgataataaag 6240
TGCTGCACCTACGCTAGCACGGGGTCAGAAAAGAGTTTCTACTAAGATAACTATTATTTC
6241 tgttgacaagatccgataaaaatagagggaagagtgataacgtcccctcagaagaagttg 6300
ACAACTGTTCTAGGCTATTTTTATCTCCCTTCTCACTATTGCAGGGGAGTCTTCTTCAAC
6301 tcaagaaaatgaaaaattattggcggcagctgctgaacgccaaactgatcacacaacgga 6360
AGTTCTTTTACTTTTTAATAACCGCCGTCGACGACTTGCGGTTTGACTAGTGTGTTGCCT
6361 agttcgataatctgactaaggctgaacgaggtggcctgtctgagttggataaagccggct 6420
TCAAGCTATTAGACTGATTCCGACTTGCTCCACCGGACAGACTCAACCTATTTCGGCCGA
6421 tcatcaaaaggcagcttgttgagacacgccagatcaccaagcacgtggcccaaattctcg 6480
AGTAGTTTTCCGTCGAACAACTCTGTGCGGTCTAGTGGTTCGTGCACCGGGTTTAAGAGC
6481 attcacgcatgaacaccaagtacgatgaaaatgacaaactgattcgagaggtgaaagtta 6540
TAAGTGCGTACTTGTGGTTCATGCTACTTTTACTGTTTGACTAAGCTCTCCACTTTCAAT
6541 ttactctgaagtctaagctggtctcagatttcagaaaggactttcagttttataaggtga 6600
AATGAGACTTCAGATTCGACCAGAGTCTAAAGTCTTTCCTGAAAGTCAAAATATTCCACT
6601 gagagatcaacaattaccaccatgcgcatgatgcctacctgaatgcagtggtaggcactg 6660
CTCTCTAGTTGTTAATGGTGGTACGCGTACTACGGATGGACTTACGTCACCATCCGTGAC
6661 cacttatcaaaaaatatcccaagcttgaatctgaatttgtttacggagactataaagtgt 6720
GTGAATAGTTTTTTATAGGGTTCGAACTTAGACTTAAACAAATGCCTCTGATATTTCACA
6721 acgatgttaggaaaatgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagt 6780
TGCTACAATCCTTTTACTAGCGTTTCAGACTCGTCCTTTATCCGTTCCGGTGGCGATTCA
6781 acttcttttacagcaatattatgaattttttcaagaccgagattacactggccaatggag 6840
TGAAGAAAATGTCGTTATAATACTTAAAAAAGTTCTGGCTCTAATGTGACCGGTTACCTC
6841 agattcggaagcgaccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggaca 6900
TCTAAGCCTTCGCTGGTGAATAGCTTTGTTTGCCTCTTTGTCCTCTTTAGCACACCCTGT
6901 agggtagggatttcgcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgtta 6960
TCCCATCCCTAAAGCGCTGTCAGGCCTTCCAGGACAGGTACGGCGTCCACTTGTAGCAAT
6961 aaaagaccgaagtacagaccggaggcttctccaaggaaagtatcctcccgaaaaggaaca 7020
TTTTCTGGCTTCATGTCTGGCCTCCGAAGAGGTTCCTTTCATAGGAGGGCTTTTCCTTGT
7021 gcgacaagctgatcgcacgcaaaaaagattgggaccccaagaaatacggcggattcgatt 7080
CGCTGTTCGACTAGCGTGCGTTTTTTCTAACCCTGGGGTTCTTTATGCCGCCTAAGCTAA
7081 ctcctacagtcgcttacagtgtactggttgtggccaaagtggagaaagggaagtctaaaa 7140
GAGGATGTCAGCGAATGTCACATGACCAACACCGGTTTCACCTCTTTCCCTTCAGATTTT
7141 aactcaaaagcgtcaaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaa 7200
TTGAGTTTTCGCAGTTCCTTGACGACCCGTAGTGTTAGTACCTCGCTAGTTCGAAGCTTT
7201 aaaaccccatcgactttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatca 7260
TTTTGGGGTAGCTGAAAGAGCTCCGCTTTCCTATATTTCTCCAGTTTTTTCTGGAGTAGT
7261 ttaagcttcccaagtactctctctttgagcttgaaaacggccggaaacgaatgctcgcta 7320
AATTCGAAGGGTTCATGAGAGAGAAACTCGAACTTTTGCCGGCCTTTGCTTACGAGCGAT
7321 gtgcgggcgagctgcagaaaggtaacgagctggcactgccctctaaatacgttaatttct 7380
CACGCCCGCTCGACGTCTTTCCATTGCTCGACCGTGACGGGAGATTTATGCAATTAAAGA
7381 tgtatctggccagccactatgaaaagctcaaagggtctcccgaagataatgagcagaagc 7440
ACATAGACCGGTCGGTGATACTTTTCGAGTTTCCCAGAGGGCTTCTATTACTCGTCTTCG
EcoRI
|
7441 agctgttcgtggaacaacacaaacactaccttgatgagatcatcgagcaaataagcgaat 7500
TCGACAAGCACCTTGTTGTGTTTGTGATGGAACTACTCTAGTAGCTCGTTTATTCGCTTA
7501 tctccaaaagagtgatcctcgccgacgctaacctcgataaggtgctttctgcttacaata 7560
AGAGGTTTTCTCACTAGGAGCGGCTGCGATTGGAGCTATTCCACGAAAGACGAATGTTAT
7561 agcacagggataagcccatcagggagcaggcagaaaacattatccacttgtttactctga 7620
TCGTGTCCCTATTCGGGTAGTCCCTCGTCCGTCTTTTGTAATAGGTGAACAAATGAGACT
7621 ccaacttgggcgcgcctgcagccttcaagtacttcgacaccaccatagacagaaagcggt 7680
GGTTGAACCCGCGCGGACGTCGGAAGTTCATGAAGCTGTGGTGGTATCTGTCTTTCGCCA
7681 acacctctacaaaggaggtcctggacgccacactgattcatcagtcaattacggggctct 7740
TGTGGAGATGTTTCCTCCAGGACCTGCGGTGTGACTAAGTAGTCAGTTAATGCCCCGAGA
7741 atgaaacaagaatcgacctctctcagctcggtggagacagcagggctgaccccaagaaga 7800
TACTTTGTTCTTAGCTGGAGAGAGTCGAGCCACCTCTGTCGTCCCGACTGGGGTTCTTCT
7801 agaggaaggtggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccg 7860
TCTCCTTCCACCGGGGTTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGTCGGC
NotI
|
7861 cggccgcccggacactggtgaccttcaaggatgtatttgtggacttcaccagggaggagt 7920
GCCGGCGGGCCTGTGACCACTGGAAGTTCCTACATAAACACCTGAAGTGGTCCCTCCTCA
7921 ggaagctgctggacactgctcagcagatcgtgtacagaaatgtgatgctggagaactata 7980
CCTTCGACGACCTGTGACGAGTCGTCTAGCACATGTCTTTACACTACGACCTCTTGATAT
7981 agaacctggtttccttgggttatcagcttactaagccagatgtgatcctccggttggaga 8040
TCTTGGACCAAAGGAACCCAATAGTCGAATGATTCGGTCTACACTAGGAGGCCAACCTCT
SV40 prom(8099,8367)>>>
|
8041 agggagaagagcccactagtccaaaaaagaaaagaaaggtgtgagtttaaacacgcgtgg 8100
TCCCTCTTCTCGGGTGATCAGGTTTTTTCTTTTCTTTCCACACTCAAATTTGTGCGCACC
8101 tgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattag 8160
ACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATC
8161 tcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatg 8220
AGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTAC
SV40 origin(8266,8343)>>>
|
8221 catctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaact 8280
GTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGA
ORF_2 rf(2)(8333,9001)>>>
|
8281 ccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagag 8340
GGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTC
SfiI
|
8341 gccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggc 8400
CGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCG
puro marker(8402,9001)>>>
|
8401 catgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgt 8460
GTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCA
8461 acgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccgga 8520
TGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCT
8521 ccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcga 8580
GGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCT
8581 catcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccgga 8640
GTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCT
8641 gagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcgg 8700
CTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCC
8701 ttcccggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaagga 8760
AAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCT
8761 gcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctggg 8820
CGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCC
8821 cagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcct 8880
GTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGA
8881 ggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgc 8940
CCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCG
8941 cgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctg 9000
GCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGAC
9001 aacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattct 9060
TTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGA
9061 taactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgc 9120
ATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACG
9121 tattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctct 9180
ATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGA
9181 ttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctga 9240
AATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACT
9241 cgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgc 9300
GCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCG
9301 tttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggac 9360
AAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTG
9361 aggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctt 9420
TCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAA
9421 tccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgt 9480
AGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCA
9481 cccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcc 9540
GGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGG
9541 tcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccg 9600
AGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGC
delta_U3 other(9611,9663)>>>
|
9601 cctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgt 9660
GGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACA
HIV-1_5_LTR other(9664,9844)>>>
|
9661 actgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaac 9720
TGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTG
9721 ccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctg 9780
GGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGAC
9781 ttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctct 9840
AACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGA
9841 agcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaata 9900
TCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTAT
9901 tcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagc 9960
AGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCG
9961 atcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaa 10020
TAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTT
10021 ctcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatga 10080
GAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACT
10081 aatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaa 10140
TTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTT
10141 ataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctc 10200
TATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAG
10201 actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg 10260
TGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGC
10261 gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc 10320
CATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCG
pBR322 origin(10350,10969)<<<
|
10321 cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc 10380
GTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCG
10381 ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga 10440
GGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCT
10441 ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc 10500
GATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGG
10501 ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcat 10560
GACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTA
10561 agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg 10620
TCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACAC
10621 cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc 10680
GTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGG
10681 aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga 10740
TTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCT
10741 gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact 10800
CGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGA
10801 agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt 10860
TCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAA
10861 ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag 10920
CCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTC
10921 cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg 10980
GTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCC
10981 tctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa 11040
AGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTT
11041 aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata 11100
TCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATAT
11101 tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcg 11160
ATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGC
11161 atctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgata 11220
TAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTAT
11221 cgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccg 11280
GCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGC
11281 gctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcct 11340
CGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGA
11341 gcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagt 11400
CGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCA
11401 tcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttca 11460
AGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGT
11461 cgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggcta 11520
GCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGAT
11521 tctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacat 11580
AGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTA
NEOKAN prom(11622,11671)>>>
|
11581 ggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctgggg 11640
CCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCC
11641 cgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaa 11700
GCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTT
11701 ggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgca 11760
CCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGT
NTP_II marker(11763,12551)>>>
|
ORF_4 rf(3)(11760,12554)>>>
| |
11761 tgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcg 11820
ACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGC
11821 gctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcag 11880
CGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTC
11881 cgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgc 11940
GCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACG
11941 aagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgc 12000
TTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACG
12001 tcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcagg 12060
AGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCC
12061 atctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgc 12120
TAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACG
12121 ggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgca 12180
CCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGT
12181 tcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaag 12240
AGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTC
12241 agcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacg 12300
TCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGC
12301 gcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatg 12360
CGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTAC
12361 gccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggaca 12420
CGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGT
12421 tagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcc 12480
ATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGG
12481 tcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttg 12540
AGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAAC
12541 acgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaatag 12600
TGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATC
f1 origin(12659,12964)<<<
|
12601 gccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgtt 12660
CGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAA
12661 gttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcga 12720
CAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCT
12721 aaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttg 12780
TTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAAC
12781 cggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagct 12840
GCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGA
12841 tgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggc 12900
ACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCG
12901 gctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgctta 12960
CGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAAT
12961 atgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaaca 13020
TACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGT
13021 tcatcaataatatacctt 13038
AGTAGTTATTATATGGAA
LOCUS dna 13038 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(6)"
Promoter 2444..3621
/gene="EF1a prom"
misc_binding 3056..3061
/dbxref="REBASE:ApaI"
ORF 3675..8084
/sequence="ORF_3 rf(3)"
misc_binding 4152..4157
/dbxref="REBASE:NdeI"
misc_binding 5041..5046
/dbxref="REBASE:SmaI"
misc_binding 5041..5046
/dbxref="REBASE:XmaI"
misc_binding 5680..5685
/dbxref="REBASE:BamHI"
misc_binding 7497..7502
/dbxref="REBASE:EcoRI"
misc_binding 7860..7867
/dbxref="REBASE:NotI"
Promoter 8099..8367
/gene="SV40 prom"
Rep_Origin 8266..8343
/gene="SV40 origin"
ORF 8333..9001
/sequence="ORF_2 rf(2)"
misc_binding 8346..8358
/dbxref="REBASE:SfiI"
Marker 8402..9001
/gene="puro marker"
Other Gene 9611..9663
/gene="delta_U3 other"
Other Gene 9664..9844
/gene="HIV-1_5_LTR other"
Rep_Origin 10350..10969
/gene="pBR322 origin"
Promoter 11622..11671
/gene="NEOKAN prom"
ORF 11760..12554
/sequence="ORF_4 rf(3)"
Marker 11763..12551
/gene="NTP_II marker"
Rep_Origin 12659..12964
/gene="f1 origin"
BASE COUNT 3441 a 3147 c 3521 g 2929 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagaa ataattttgt ttaactttaa
3661 gaaggagata taccatggac aagaagtact ccattgggct cgcgatcggc acaaacagcg
3721 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
3781 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
3841 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
3901 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
3961 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
4021 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
4081 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
4141 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
4201 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
4261 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
4321 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
4381 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
4441 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
4501 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
4561 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
4621 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
4681 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
4741 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
4801 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
4861 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
4921 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
4981 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
5041 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
5101 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
5161 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
5221 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
5281 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
5341 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
5401 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
5461 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
5521 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
5581 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
5641 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
5701 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
5761 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
5821 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
5881 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
5941 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
6001 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
6061 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
6121 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
6181 acgacgtgga tgcgatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
6241 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
6301 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
6361 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
6421 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
6481 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
6541 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
6601 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
6661 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
6721 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
6781 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
6841 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
6901 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
6961 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
7021 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
7081 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
7141 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
7201 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
7261 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
7321 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
7381 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
7441 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
7501 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
7561 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
7621 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
7681 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
7741 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
7801 agaggaaggt ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
7861 cggccgcccg gacactggtg accttcaagg atgtatttgt ggacttcacc agggaggagt
7921 ggaagctgct ggacactgct cagcagatcg tgtacagaaa tgtgatgctg gagaactata
7981 agaacctggt ttccttgggt tatcagctta ctaagccaga tgtgatcctc cggttggaga
8041 agggagaaga gcccactagt ccaaaaaaga aaagaaaggt gtgagtttaa acacgcgtgg
8101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
8161 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
8221 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
8281 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
8341 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
8401 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt
8461 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga
8521 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
8581 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
8641 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
8701 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga
8761 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
8821 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
8881 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
8941 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
9001 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
9061 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
9121 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
9181 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
9241 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
9301 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
9361 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
9421 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
9481 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
9541 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
9601 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt
9661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
9721 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
9781 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
9841 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
9901 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
9961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
10021 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga
10081 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa
10141 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
10201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
10261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
10321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
10381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
10441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
10501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
10561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
10621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
10681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
10741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
10801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
10861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
10921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
10981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
11041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
11101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
11161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
11221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
11281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
11341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
11401 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca
11461 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
11521 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
11581 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
11641 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa
11701 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
11761 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
11821 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
11881 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
11941 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
12001 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
12061 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
12121 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
12181 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
12241 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
12301 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
12361 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
12421 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
12481 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
12541 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag
12601 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt
12661 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
12721 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg
12781 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct
12841 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
12901 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta
12961 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca
13021 tcatcaataa tatacctt
//