pLenti-EF1a-hTERT-Hygro
| Vector Name | pLenti-EF1a-hTERT-Hygro |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | EF1a forward sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5'--TAG TCA GCC ATG GGG CGG AGA--3' |
| Additional Information | The gene expression is driven by an EF1a promoter, without any Tag or reporter. |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagcg atatcacaag tttgtacaaa
3661 aaagttggca tgccgcgcgc tccccgctgc cgagccgtgc gctccctgct gcgcagccac
3721 taccgcgagg tgctgccgct ggccacgttc gtgcggcgcc tggggcccca gggctggcgg
3781 ctggtgcagc gcggggaccc ggcggctttc cgcgcgctgg tggcccagtg cctggtgtgc
3841 gtgccctggg acgcacggcc gccccccgcc gccccctcct tccgccaggt gtcctgcctg
3901 aaggagctgg tggcccgagt gctgcagagg ctgtgcgagc gcggcgcgaa gaacgtgctg
3961 gccttcggct tcgcgctgct ggacggggcc cgcgggggcc cccccgaggc cttcaccacc
4021 agcgtgcgca gctacctgcc caacacggtg accgacgcac tgcgggggag cggggcgtgg
4081 gggctgctgc tgcgccgcgt gggcgacgac gtgctggttc acctgctggc acgctgcgcg
4141 ctctttgtgc tggtggctcc cagctgcgcc taccaggtgt gcgggccgcc gctgtaccag
4201 ctcggcgctg ccactcaggc ccggcccccg ccacacgcta gtggaccccg aaggcgtctg
4261 ggatgcgaac gggcctggaa ccatagcgtc agggaggccg gggtccccct gggcctgcca
4321 gccccgggtg cgaggaggcg cgggggcagt gccagccgaa gtctgccgtt gcccaagagg
4381 cccaggcgtg gcgctgcccc tgagccggag cggacgcccg ttgggcaggg gtcctgggcc
4441 cacccgggca ggacgcgtgg accgagtgac cgtggtttct gtgtggtgtc acctgccaga
4501 cccgccgaag aagccacctc tttggagggt gcgctctctg gcacgcgcca ctcccaccca
4561 tccgtgggcc gccagcacca cgcgggcccc ccatccacat cgcggccacc acgtccctgg
4621 gacacgcctt gtcccccggt gtacgccgag accaagcact tcctctactc ctcaggcgac
4681 aaggagcagc tgcggccctc cttcctactc agctctctga ggcccagcct gactggcgct
4741 cggaggctcg tggagaccat ctttctgggt tccaggccct ggatgccagg gactccccgc
4801 aggttgcccc gcctgcccca gcgctactgg caaatgcggc ccctgtttct ggagctgctt
4861 gggaaccacg cgcagtgccc ctacggggtg ctcctcaaga cgcactgccc gctgcgagct
4921 gcggtcaccc cagcagccgg tgtctgtgcc cgggagaagc cccagggctc tgtggcggcc
4981 cccgaggagg aggacacaga cccccgtcgc ctggtgcagc tgctccgcca gcacagcagc
5041 ccctggcagg tgtacggctt cgtgcgggcc tgcctgcgcc ggctggtgcc cccaggcctc
5101 tggggctcca ggcacaacga acgccgcttc ctcaggaaca ccaagaagtt catctccctg
5161 gggaagcatg ccaagctctc gctgcaggag ctgacgtgga agatgagcgt gcgggactgc
5221 gcttggctgc gcaggagccc aggggttggc tgtgttccgg ccgcagagca ccgtctgcgt
5281 gaggagatcc tggccaagtt cctgcactgg ctgatgagtg tgtacgtcgt cgagctgctc
5341 aggtctttct tttatgtcac ggagaccacg tttcaaaaga acaggctctt tttctaccgg
5401 aagagtgtct ggagcaagtt gcaaagcatt ggaatcagac agcacttgaa gagggtgcag
5461 ctgcgggagc tgtcggaagc agaggtcagg cagcatcggg aagccaggcc cgccctgctg
5521 acgtccagac tccgcttcat ccccaagcct gacgggctgc ggccgattgt gaacatggac
5581 tacgtcgtgg gagccagaac gttccgcaga gaaaagaggg ccgagcgtct cacctcgagg
5641 gtgaaggcac tgttcagcgt gctcaactac gagcgggcgc ggcgccccgg cctcctgggc
5701 gcctctgtgc tgggcctgga cgatatccac agggcctggc gcaccttcgt gctgcgtgtg
5761 cgggcccagg acccgccgcc tgagctgtac tttgtcaagg tggatgtgac gggcgcgtac
5821 gacaccatcc cccaggacag gctcacggag gtcatcgcca gcatcatcaa accccagaac
5881 acgtactgcg tgcgtcggta tgccgtggtc cagaaggccg cccatgggca cgtccgcaag
5941 gccttcaaga gccacgtctc taccttgaca gacctccagc cgtacatgcg acagttcgtg
6001 gctcacctgc aggagaccag cccgctgagg gatgccgtcg tcatcgagca gagctcctcc
6061 ctgaatgagg ccagcagtgg cctcttcgac gtcttcctac gcttcatgtg ccaccacgcc
6121 gtgcgcatca ggggcaagtc ctacgtccag tgccagggga tcccgcaggg ctccatcctc
6181 tccacgctgc tctgcagcct gtgctacggc gacatggaga acaagctgtt tgcggggatt
6241 cggcgggacg ggctgctcct gcgtttggtg gatgatttct tgttggtgac acctcacctc
6301 acccacgcga aaaccttcct caggaccctg gtccgaggtg tccctgagta tggctgcgtg
6361 gtgaacttgc ggaagacagt ggtgaacttc cctgtagaag acgaggccct gggtggcacg
6421 gcttttgttc agatgccggc ccacggccta ttcccctggt gcggcctgct gctggatacc
6481 cggaccctgg aggtgcagag cgactactcc agctatgccc ggacctccat cagagccagt
6541 ctcaccttca accgcggctt caaggctggg aggaacatgc gtcgcaaact ctttggggtc
6601 ttgcggctga agtgtcacag cctgtttctg gatttgcagg tgaacagcct ccagacggtg
6661 tgcaccaaca tctacaagat cctcctgctg caggcgtaca ggtttcacgc atgtgtgctg
6721 cagctcccat ttcatcagca agtttggaag aaccccacat ttttcctgcg cgtcatctct
6781 gacacggcct ccctctgcta ctccatcctg aaagccaaga acgcagggat gtcgctgggg
6841 gccaagggcg ccgccggccc tctgccctcc gaggccgtgc agtggctgtg ccaccaagca
6901 ttcctgctca agctgactcg acaccgtgtc acctacgtgc cactcctggg gtcactcagg
6961 acagcccaga cgcagctgag tcggaagctc ccggggacga cgctgactgc cctggaggcc
7021 gcagccaacc cggcactgcc ctcagacttc aagaccatcc tggactgccc aactttcttg
7081 tacaaagtgg tggatatctg aggatcctct agactgcagc tcgagtaccc atacgacgtc
7141 ccagactacg cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg
7201 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
7261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
7321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
7381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
7441 ccagaagtag tgaggaggct tttttggagg ccatgaaaaa gcctgaactc accgcgacgt
7501 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
7561 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
7621 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
7681 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
7741 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
7801 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
7861 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
7921 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
7981 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
8041 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
8101 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
8161 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
8221 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
8281 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
8341 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
8401 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
8461 accgacgccc cagcactcgt ccgagggcaa aggaatagac gcgttccgga aatcaacctc
8521 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
8581 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
8641 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
8701 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
8761 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
8821 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
8881 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
8941 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
9001 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc
9061 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat
9121 tcactcccaa cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca
9181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
9241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
9301 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
9361 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
9421 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
9481 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
9541 ctggcatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg
9601 tctgcattaa tgaatcggcc aacgatcccg gtgtgaaata ccgcacagat gcgtaaggag
9661 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
9721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
9781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
9841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
9901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
9961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
10021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
10081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
10141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
10201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
10261 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
10321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
10381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
10441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
10501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
10561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
10621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
10861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
10921 cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa
10981 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
11041 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
11101 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
11161 ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca
11221 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
11281 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
11341 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
11401 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
11461 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
11521 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
11581 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
11641 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
11701 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
11761 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
11821 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
11881 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
11941 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
12001 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattttgtta
12061 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca acatccctta
12121 taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga acaagagtcc
12181 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
12241 cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct
12301 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
12361 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
12421 ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca gggcgcgtcc
12481 attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat acctt
LOCUS dna 12535 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(4)"
Promoter 2444..3621
/gene="EF1a prom"
ORF 3433..4284
/sequence="ORF_7 rf(5)"
ORF 3670..7101
/sequence="ORF_2 rf(1)"
ORF 4705..5925
/sequence="ORF_6 rf(5)"
misc_binding 7108..7113
/dbxref="REBASE:XbaI"
Promoter 7170..7438
/gene="SV40 prom"
Rep_Origin 7337..7414
/gene="SV40 origin"
ORF 7398..8231
/sequence="ORF_8 rf(6)"
ORF 7404..8498
/sequence="ORF_4 rf(3)"
misc_binding 7417..7429
/dbxref="REBASE:SfiI"
Marker 7478..8495
/gene="hygroB marker"
misc_binding 7716..7721
/dbxref="REBASE:EcoRI"
misc_binding 7922..7927
/dbxref="REBASE:NdeI"
Other Gene 9108..9160
/gene="delta_U3 other"
Other Gene 9161..9341
/gene="HIV-1_5_LTR other"
Rep_Origin 9847..10466
/gene="pBR322 origin"
Promoter 11119..11168
/gene="NEOKAN prom"
misc_binding 11226..11231
/dbxref="REBASE:BclI"
ORF 11257..12051
/sequence="ORF_3 rf(1)"
Marker 11260..12048
/gene="NTP_II marker"
Rep_Origin 12156..12461
/gene="f1 origin"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
ORF_5 rf(4)(2381,3361)<<<
|
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
EF1a prom(2444,3621)>>>
|
2401 aattcaaaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtca 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGT
2461 gtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattg 2520
CACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAAC
2521 aaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggct 2580
TTGGCCACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGA
2581 ccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgt 2640
GGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCA
2641 tctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgg 2700
AGAAAAAGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCC
2701 gcctggcctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcag 2760
CGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTC
2761 tacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgc 2820
ATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACG
2821 gcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgc 2880
CGAATTCCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCG
2881 cgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagcc 2940
GCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGG
2941 atttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaat 3000
TAAATTTTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTA
3001 gcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcc 3060
CGCCCGGTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGG
3061 cgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatc 3120
GCACGCAGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAG
3121 ggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgt 3180
CCTGCCCCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACA
3181 atcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaaga 3240
TAGCGGGGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCT
3241 tggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagag 3300
ACCGGCGAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTC
3301 cgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttca 3360
GCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGT
3361 tgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttgg 3420
ACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACC
ORF_7 rf(5)(3433,4284)<<<
|
3421 agtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgag 3480
TCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTC
3481 tgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcc 3540
ACCCACCTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGG
3541 ctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttttttt 3600
GAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAA
3601 cttccatttcaggtgtcgtgaggaatttcgactgctagcgatatcacaagtttgtacaaa 3660
GAAGGTAAAGTCCACAGCACTCCTTAAAGCTGACGATCGCTATAGTGTTCAAACATGTTT
ORF_2 rf(1)(3670,7101)>>>
|
3661 aaagttggcatgccgcgcgctccccgctgccgagccgtgcgctccctgctgcgcagccac 3720
TTTCAACCGTACGGCGCGCGAGGGGCGACGGCTCGGCACGCGAGGGACGACGCGTCGGTG
3721 taccgcgaggtgctgccgctggccacgttcgtgcggcgcctggggccccagggctggcgg 3780
ATGGCGCTCCACGACGGCGACCGGTGCAAGCACGCCGCGGACCCCGGGGTCCCGACCGCC
3781 ctggtgcagcgcggggacccggcggctttccgcgcgctggtggcccagtgcctggtgtgc 3840
GACCACGTCGCGCCCCTGGGCCGCCGAAAGGCGCGCGACCACCGGGTCACGGACCACACG
3841 gtgccctgggacgcacggccgccccccgccgccccctccttccgccaggtgtcctgcctg 3900
CACGGGACCCTGCGTGCCGGCGGGGGGCGGCGGGGGAGGAAGGCGGTCCACAGGACGGAC
3901 aaggagctggtggcccgagtgctgcagaggctgtgcgagcgcggcgcgaagaacgtgctg 3960
TTCCTCGACCACCGGGCTCACGACGTCTCCGACACGCTCGCGCCGCGCTTCTTGCACGAC
3961 gccttcggcttcgcgctgctggacggggcccgcgggggcccccccgaggccttcaccacc 4020
CGGAAGCCGAAGCGCGACGACCTGCCCCGGGCGCCCCCGGGGGGGCTCCGGAAGTGGTGG
4021 agcgtgcgcagctacctgcccaacacggtgaccgacgcactgcgggggagcggggcgtgg 4080
TCGCACGCGTCGATGGACGGGTTGTGCCACTGGCTGCGTGACGCCCCCTCGCCCCGCACC
4081 gggctgctgctgcgccgcgtgggcgacgacgtgctggttcacctgctggcacgctgcgcg 4140
CCCGACGACGACGCGGCGCACCCGCTGCTGCACGACCAAGTGGACGACCGTGCGACGCGC
4141 ctctttgtgctggtggctcccagctgcgcctaccaggtgtgcgggccgccgctgtaccag 4200
GAGAAACACGACCACCGAGGGTCGACGCGGATGGTCCACACGCCCGGCGGCGACATGGTC
4201 ctcggcgctgccactcaggcccggcccccgccacacgctagtggaccccgaaggcgtctg 4260
GAGCCGCGACGGTGAGTCCGGGCCGGGGGCGGTGTGCGATCACCTGGGGCTTCCGCAGAC
4261 ggatgcgaacgggcctggaaccatagcgtcagggaggccggggtccccctgggcctgcca 4320
CCTACGCTTGCCCGGACCTTGGTATCGCAGTCCCTCCGGCCCCAGGGGGACCCGGACGGT
4321 gccccgggtgcgaggaggcgcgggggcagtgccagccgaagtctgccgttgcccaagagg 4380
CGGGGCCCACGCTCCTCCGCGCCCCCGTCACGGTCGGCTTCAGACGGCAACGGGTTCTCC
4381 cccaggcgtggcgctgcccctgagccggagcggacgcccgttgggcaggggtcctgggcc 4440
GGGTCCGCACCGCGACGGGGACTCGGCCTCGCCTGCGGGCAACCCGTCCCCAGGACCCGG
4441 cacccgggcaggacgcgtggaccgagtgaccgtggtttctgtgtggtgtcacctgccaga 4500
GTGGGCCCGTCCTGCGCACCTGGCTCACTGGCACCAAAGACACACCACAGTGGACGGTCT
4501 cccgccgaagaagccacctctttggagggtgcgctctctggcacgcgccactcccaccca 4560
GGGCGGCTTCTTCGGTGGAGAAACCTCCCACGCGAGAGACCGTGCGCGGTGAGGGTGGGT
4561 tccgtgggccgccagcaccacgcgggccccccatccacatcgcggccaccacgtccctgg 4620
AGGCACCCGGCGGTCGTGGTGCGCCCGGGGGGTAGGTGTAGCGCCGGTGGTGCAGGGACC
4621 gacacgccttgtcccccggtgtacgccgagaccaagcacttcctctactcctcaggcgac 4680
CTGTGCGGAACAGGGGGCCACATGCGGCTCTGGTTCGTGAAGGAGATGAGGAGTCCGCTG
ORF_6 rf(5)(4705,5925)<<<
|
4681 aaggagcagctgcggccctccttcctactcagctctctgaggcccagcctgactggcgct 4740
TTCCTCGTCGACGCCGGGAGGAAGGATGAGTCGAGAGACTCCGGGTCGGACTGACCGCGA
4741 cggaggctcgtggagaccatctttctgggttccaggccctggatgccagggactccccgc 4800
GCCTCCGAGCACCTCTGGTAGAAAGACCCAAGGTCCGGGACCTACGGTCCCTGAGGGGCG
4801 aggttgccccgcctgccccagcgctactggcaaatgcggcccctgtttctggagctgctt 4860
TCCAACGGGGCGGACGGGGTCGCGATGACCGTTTACGCCGGGGACAAAGACCTCGACGAA
4861 gggaaccacgcgcagtgcccctacggggtgctcctcaagacgcactgcccgctgcgagct 4920
CCCTTGGTGCGCGTCACGGGGATGCCCCACGAGGAGTTCTGCGTGACGGGCGACGCTCGA
4921 gcggtcaccccagcagccggtgtctgtgcccgggagaagccccagggctctgtggcggcc 4980
CGCCAGTGGGGTCGTCGGCCACAGACACGGGCCCTCTTCGGGGTCCCGAGACACCGCCGG
4981 cccgaggaggaggacacagacccccgtcgcctggtgcagctgctccgccagcacagcagc 5040
GGGCTCCTCCTCCTGTGTCTGGGGGCAGCGGACCACGTCGACGAGGCGGTCGTGTCGTCG
5041 ccctggcaggtgtacggcttcgtgcgggcctgcctgcgccggctggtgcccccaggcctc 5100
GGGACCGTCCACATGCCGAAGCACGCCCGGACGGACGCGGCCGACCACGGGGGTCCGGAG
5101 tggggctccaggcacaacgaacgccgcttcctcaggaacaccaagaagttcatctccctg 5160
ACCCCGAGGTCCGTGTTGCTTGCGGCGAAGGAGTCCTTGTGGTTCTTCAAGTAGAGGGAC
5161 gggaagcatgccaagctctcgctgcaggagctgacgtggaagatgagcgtgcgggactgc 5220
CCCTTCGTACGGTTCGAGAGCGACGTCCTCGACTGCACCTTCTACTCGCACGCCCTGACG
5221 gcttggctgcgcaggagcccaggggttggctgtgttccggccgcagagcaccgtctgcgt 5280
CGAACCGACGCGTCCTCGGGTCCCCAACCGACACAAGGCCGGCGTCTCGTGGCAGACGCA
5281 gaggagatcctggccaagttcctgcactggctgatgagtgtgtacgtcgtcgagctgctc 5340
CTCCTCTAGGACCGGTTCAAGGACGTGACCGACTACTCACACATGCAGCAGCTCGACGAG
5341 aggtctttcttttatgtcacggagaccacgtttcaaaagaacaggctctttttctaccgg 5400
TCCAGAAAGAAAATACAGTGCCTCTGGTGCAAAGTTTTCTTGTCCGAGAAAAAGATGGCC
5401 aagagtgtctggagcaagttgcaaagcattggaatcagacagcacttgaagagggtgcag 5460
TTCTCACAGACCTCGTTCAACGTTTCGTAACCTTAGTCTGTCGTGAACTTCTCCCACGTC
5461 ctgcgggagctgtcggaagcagaggtcaggcagcatcgggaagccaggcccgccctgctg 5520
GACGCCCTCGACAGCCTTCGTCTCCAGTCCGTCGTAGCCCTTCGGTCCGGGCGGGACGAC
5521 acgtccagactccgcttcatccccaagcctgacgggctgcggccgattgtgaacatggac 5580
TGCAGGTCTGAGGCGAAGTAGGGGTTCGGACTGCCCGACGCCGGCTAACACTTGTACCTG
5581 tacgtcgtgggagccagaacgttccgcagagaaaagagggccgagcgtctcacctcgagg 5640
ATGCAGCACCCTCGGTCTTGCAAGGCGTCTCTTTTCTCCCGGCTCGCAGAGTGGAGCTCC
5641 gtgaaggcactgttcagcgtgctcaactacgagcgggcgcggcgccccggcctcctgggc 5700
CACTTCCGTGACAAGTCGCACGAGTTGATGCTCGCCCGCGCCGCGGGGCCGGAGGACCCG
5701 gcctctgtgctgggcctggacgatatccacagggcctggcgcaccttcgtgctgcgtgtg 5760
CGGAGACACGACCCGGACCTGCTATAGGTGTCCCGGACCGCGTGGAAGCACGACGCACAC
5761 cgggcccaggacccgccgcctgagctgtactttgtcaaggtggatgtgacgggcgcgtac 5820
GCCCGGGTCCTGGGCGGCGGACTCGACATGAAACAGTTCCACCTACACTGCCCGCGCATG
5821 gacaccatcccccaggacaggctcacggaggtcatcgccagcatcatcaaaccccagaac 5880
CTGTGGTAGGGGGTCCTGTCCGAGTGCCTCCAGTAGCGGTCGTAGTAGTTTGGGGTCTTG
5881 acgtactgcgtgcgtcggtatgccgtggtccagaaggccgcccatgggcacgtccgcaag 5940
TGCATGACGCACGCAGCCATACGGCACCAGGTCTTCCGGCGGGTACCCGTGCAGGCGTTC
5941 gccttcaagagccacgtctctaccttgacagacctccagccgtacatgcgacagttcgtg 6000
CGGAAGTTCTCGGTGCAGAGATGGAACTGTCTGGAGGTCGGCATGTACGCTGTCAAGCAC
6001 gctcacctgcaggagaccagcccgctgagggatgccgtcgtcatcgagcagagctcctcc 6060
CGAGTGGACGTCCTCTGGTCGGGCGACTCCCTACGGCAGCAGTAGCTCGTCTCGAGGAGG
6061 ctgaatgaggccagcagtggcctcttcgacgtcttcctacgcttcatgtgccaccacgcc 6120
GACTTACTCCGGTCGTCACCGGAGAAGCTGCAGAAGGATGCGAAGTACACGGTGGTGCGG
6121 gtgcgcatcaggggcaagtcctacgtccagtgccaggggatcccgcagggctccatcctc 6180
CACGCGTAGTCCCCGTTCAGGATGCAGGTCACGGTCCCCTAGGGCGTCCCGAGGTAGGAG
6181 tccacgctgctctgcagcctgtgctacggcgacatggagaacaagctgtttgcggggatt 6240
AGGTGCGACGAGACGTCGGACACGATGCCGCTGTACCTCTTGTTCGACAAACGCCCCTAA
6241 cggcgggacgggctgctcctgcgtttggtggatgatttcttgttggtgacacctcacctc 6300
GCCGCCCTGCCCGACGAGGACGCAAACCACCTACTAAAGAACAACCACTGTGGAGTGGAG
6301 acccacgcgaaaaccttcctcaggaccctggtccgaggtgtccctgagtatggctgcgtg 6360
TGGGTGCGCTTTTGGAAGGAGTCCTGGGACCAGGCTCCACAGGGACTCATACCGACGCAC
6361 gtgaacttgcggaagacagtggtgaacttccctgtagaagacgaggccctgggtggcacg 6420
CACTTGAACGCCTTCTGTCACCACTTGAAGGGACATCTTCTGCTCCGGGACCCACCGTGC
6421 gcttttgttcagatgccggcccacggcctattcccctggtgcggcctgctgctggatacc 6480
CGAAAACAAGTCTACGGCCGGGTGCCGGATAAGGGGACCACGCCGGACGACGACCTATGG
6481 cggaccctggaggtgcagagcgactactccagctatgcccggacctccatcagagccagt 6540
GCCTGGGACCTCCACGTCTCGCTGATGAGGTCGATACGGGCCTGGAGGTAGTCTCGGTCA
6541 ctcaccttcaaccgcggcttcaaggctgggaggaacatgcgtcgcaaactctttggggtc 6600
GAGTGGAAGTTGGCGCCGAAGTTCCGACCCTCCTTGTACGCAGCGTTTGAGAAACCCCAG
6601 ttgcggctgaagtgtcacagcctgtttctggatttgcaggtgaacagcctccagacggtg 6660
AACGCCGACTTCACAGTGTCGGACAAAGACCTAAACGTCCACTTGTCGGAGGTCTGCCAC
6661 tgcaccaacatctacaagatcctcctgctgcaggcgtacaggtttcacgcatgtgtgctg 6720
ACGTGGTTGTAGATGTTCTAGGAGGACGACGTCCGCATGTCCAAAGTGCGTACACACGAC
6721 cagctcccatttcatcagcaagtttggaagaaccccacatttttcctgcgcgtcatctct 6780
GTCGAGGGTAAAGTAGTCGTTCAAACCTTCTTGGGGTGTAAAAAGGACGCGCAGTAGAGA
6781 gacacggcctccctctgctactccatcctgaaagccaagaacgcagggatgtcgctgggg 6840
CTGTGCCGGAGGGAGACGATGAGGTAGGACTTTCGGTTCTTGCGTCCCTACAGCGACCCC
6841 gccaagggcgccgccggccctctgccctccgaggccgtgcagtggctgtgccaccaagca 6900
CGGTTCCCGCGGCGGCCGGGAGACGGGAGGCTCCGGCACGTCACCGACACGGTGGTTCGT
6901 ttcctgctcaagctgactcgacaccgtgtcacctacgtgccactcctggggtcactcagg 6960
AAGGACGAGTTCGACTGAGCTGTGGCACAGTGGATGCACGGTGAGGACCCCAGTGAGTCC
6961 acagcccagacgcagctgagtcggaagctcccggggacgacgctgactgccctggaggcc 7020
TGTCGGGTCTGCGTCGACTCAGCCTTCGAGGGCCCCTGCTGCGACTGACGGGACCTCCGG
7021 gcagccaacccggcactgccctcagacttcaagaccatcctggactgcccaactttcttg 7080
CGTCGGTTGGGCCGTGACGGGAGTCTGAAGTTCTGGTAGGACCTGACGGGTTGAAAGAAC
XbaI
|
7081 tacaaagtggtggatatctgaggatcctctagactgcagctcgagtacccatacgacgtc 7140
ATGTTTCACCACCTATAGACTCCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAG
SV40 prom(7170,7438)>>>
|
7141 ccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcagg 7200
GGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCC
7201 cagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccagg 7260
GTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCC
7261 ctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtccc 7320
GAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGG
SV40 origin(7337,7414)>>>
|
7321 gcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccca 7380
CGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGT
ORF_4 rf(3)(7404,8498)>>>
|
ORF_8 rf(6)(7398,8231)<<< SfiI
| | |
7381 tggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctatt 7440
ACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAA
hygroB marker(7478,8495)>>>
|
7441 ccagaagtagtgaggaggcttttttggaggccatgaaaaagcctgaactcaccgcgacgt 7500
GGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTTTTTCGGACTTGAGTGGCGCTGCA
7501 ctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcgg 7560
GACAGCTCTTCAAAGACTAGCTTTTCAAGCTGTCGCAGAGGCTGGACTACGTCGAGAGCC
7561 agggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcggg 7620
TCCCGCTTCTTAGAGCACGAAAGTCGAAGCTACATCCTCCCGCACCTATACAGGACGCCC
7621 taaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcgg 7680
ATTTATCGACGCGGCTACCAAAGATGTTTCTAGCAATACAAATAGCCGTGAAACGTAGCC
EcoRI
|
7681 ccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctatt 7740
GGCGCGAGGGCTAAGGCCTTCACGAACTGTAACCCCTTAAGTCGCTCTCGGACTGGATAA
7741 gcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccg 7800
CGTAGAGGGCGGCACGTGTCCCACAGTGCAACGTTCTGGACGGACTTTGGCTTGACGGGC
7801 ctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccaga 7860
GACAAGACGTCGGCCAGCGCCTCCGGTACCTACGCTAGCGACGCCGGCTAGAATCGGTCT
7861 cgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatt 7920
GCTCGCCCAAGCCGGGTAAGCCTGGCGTTCCTTAGCCAGTTATGTGATGTACCGCACTAA
NdeI
|
7921 tcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccg 7980
AGTATACGCGCTAACGACTAGGGGTACACATAGTGACCGTTTGACACTACCTGCTGTGGC
7981 tcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccg 8040
AGTCACGCAGGCAGCGCGTCCGAGAGCTACTCGACTACGAAACCCGGCTCCTGACGGGGC
8041 aagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggcc 8100
TTCAGGCCGTGGAGCACGTGCGCCTAAAGCCGAGGTTGTTACAGGACTGCCTGTTACCGG
8101 gcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcg 8160
CGTATTGTCGCCAGTAACTGACCTCGCTCCGCTACAAGCCCCTAAGGGTTATGCTCCAGC
8161 ccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcg 8220
GGTTGTAGAAGAAGACCTCCGGCACCAACCGAACATACCTCGTCGTCTGCGCGATGAAGC
8221 agcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattg 8280
TCGCCTCCGTAGGCCTCGAACGTCCTAGCGGCGCCGAGGCCCGCATATACGAGGCGTAAC
8281 gtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgc 8340
CAGAACTGGTTGAGATAGTCTCGAACCAACTGCCGTTAAAGCTACTACGTCGAACCCGCG
8341 agggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcg 8400
TCCCAGCTACGCTGCGTTAGCAGGCTAGGCCTCGGCCCTGACAGCCCGCATGTGTTTAGC
8401 cccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaa 8460
GGGCGTCTTCGCGCCGGCAGACCTGGCTACCGACACATCTTCATGAGCGGCTATCACCTT
8461 accgacgccccagcactcgtccgagggcaaaggaatagacgcgttccggaaatcaacctc 8520
TGGCTGCGGGGTCGTGAGCAGGCTCCCGTTTCCTTATCTGCGCAAGGCCTTTAGTTGGAG
8521 tggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgc 8580
ACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCG
8581 tatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttca 8640
ATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGT
8641 ttttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttg 8700
AAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAAC
8701 tcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggca 8760
AGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGT
8761 ttgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacgg 8820
AACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCC
8821 cggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactg 8880
GCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGAC
8881 acaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttg 8940
TGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAAC
8941 ccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcgg 9000
GGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCC
9001 accttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccc 9060
TGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGG
delta_U3 other(9108,9160)>>>
|
9061 tcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaat 9120
AGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTA
HIV-1_5_LTR other(9161,9341)>>>
|
9121 tcactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagacca 9180
AGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGT
9181 gatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaag 9240
CTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTC
9241 cttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagag 9300
GAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTC
9301 atccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatct 9360
TAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGA
9361 tattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtt 9420
ATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAA
9421 tattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagc 9480
ATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCG
9481 atttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgt 9540
TAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACA
9541 ctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatggg 9600
GACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCC
9601 tctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggag 9660
AGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTC
9661 aaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgt 9720
TTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCA
9721 tcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatc 9780
AGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAG
9781 aggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa 9840
TCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATT
pBR322 origin(9847,10466)<<<
|
9841 aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaa 9900
TTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTT
9901 tcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcc 9960
AGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGG
9961 ccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtc 10020
GGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAG
10021 cgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcag 10080
GCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTC
10081 ttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccga 10140
AAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCT
10141 ccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatc 10200
GGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAG
10201 gccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctac 10260
CGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATG
10261 agagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctg 10320
TCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGAC
10321 cgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaaca 10380
GCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGT
10381 aaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaa 10440
TTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTT
10441 aggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaa 10500
TCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTT
10501 ctcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttt 10560
GAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAA
10561 aaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacag 10620
TTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTC
10621 ttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccat 10680
AATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTA
10681 agttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccc 10740
TCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGG
10741 cagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaa 10800
GTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTT
10801 ccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcca 10860
GGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGT
10861 gtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaa 10920
CAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTT
10921 cgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaa 10980
GCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTT
10981 cggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagc 11040
GCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCG
11041 gcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggtt 11100
CGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAA
NEOKAN prom(11119,11168)>>>
|
11101 ttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaag 11160
AATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTC
11161 ccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatca 11220
GGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGT
NTP_II marker(11260,12048)>>>
|
BclI ORF_3 rf(1)(11257,12051)>>>
| | |
11221 agctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcac 11280
TCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTG
11281 gcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagaca 11340
CGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGT
11341 atcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttcttttt 11400
TAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAA
11401 gtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcg 11460
CAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGC
11461 tggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggga 11520
ACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCT
11521 agggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgct 11580
TCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGA
11581 cctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccg 11640
GGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGC
11641 gctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatg 11700
CGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTAC
11701 gaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagcc 11760
CTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGG
11761 gaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccat 11820
CTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTA
11821 ggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgac 11880
CCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTG
11881 tgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatatt 11940
ACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAA
11941 gctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgct 12000
CGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGA
12001 cccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgtta 12060
GGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAAT
12061 aaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatccctta 12120
TTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAAT
f1 origin(12156,12461)<<<
|
12121 taaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtcc 12180
ATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGG
12181 actattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatgg 12240
TGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACC
12241 cccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctct 12300
GGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGA
12301 aaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgt 12360
TTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCA
12361 ggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagc 12420
CCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCG
12421 ggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtcc 12480
CCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGG
12481 attcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 12535
TAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA