pLenti-GIII-CMV
| Vector Name | pLenti-GIII-CMV |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | Bacterial: Kanamycin. Mammalian: Puromycin. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | The gene expression is driven by a CMV promoter, without any Tag or reporter. Original Vector: 9779bp Vector without Insert Casette: 8074bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
61 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
121 catagaagaa ccgagtttaa actccctatc agtgatagag atctccctat cagtgataga
181 gagctagcga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
241 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
301 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
361 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
421 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
481 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
541 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
601 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
661 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
721 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
781 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
841 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
901 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
961 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
1021 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
1081 gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
1141 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
1201 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
1261 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
1321 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
1381 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
1441 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
1501 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
1561 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
1621 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
1681 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
1741 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
1801 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
1861 cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatatctga ctcgagtacc
1921 catacgacgt cccagactac gcttgagttt aaacacgcgt ggtgtggaaa gtccccaggc
1981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
2041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
2101 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
2161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
2221 tctgagctat tccagaagta gtgaggaggc ttttttggag gccatgaccg agtacaagcc
2281 cacggtgcgc ctcgccaccc gcgacgacgt ccctcgggcc gtacgcaccc tcgccgccgc
2341 gttcgccgac taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt
2401 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
2461 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
2521 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
2581 gcaacagatg gaagggctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
2641 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
2701 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
2761 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
2821 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt ccggaaatca
2881 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
2941 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
3001 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
3061 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
3121 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
3181 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
3241 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
3301 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
3361 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctcgcctt
3421 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtccg gatggaaggg
3481 ctaattcact cccaacgaat acaagatctg ctttttgctt gtactgggtc tctctggtta
3541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
3601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
3661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
3721 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
3781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
3841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
3901 catgtctggc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt
3961 atgggtctgc attaatgaat cggccaacgg atgtcggtgt gaaataccgc acagatgcgt
4021 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
4681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
4741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4861 cgaaaactca cgttaaggga ttttggccat gagattatca aaaaggatct tcacctagat
4921 ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
4981 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
5041 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
5101 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
5161 gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc
5221 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
5281 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
5341 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
5401 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
5461 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
5521 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
5581 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
5641 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
5701 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
5761 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
5821 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
5881 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
5941 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
6001 ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta
6061 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
6121 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
6181 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
6241 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
6301 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
6361 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
6421 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
6481 taattaacat catcaataat ataccttttt tggattgaag ccaatatgat aatgaggggg
6541 tggagtttgt gacgtggcgc ggggcgtggg aacggggcgg gtgacgtagt agtgtggcgg
6601 aagtgtgatg ttgcaagtgt ggcggaacac atgtaagcga cggatgtggc aaaagtgacg
6661 tttttggtgt gcgccggtgt acacaggaag tgacaatttt cgcgcggttt taggcggatg
6721 ttgtagtaaa tttgggcgta accgagtaag atttggccat tttcgcggga aaactgaata
6781 agaggaagtg aaatctgaat aattttgtgt tactcatagc gcgtaatact ctcatgagcg
6841 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
6901 gaaaagtgcc acctgacgta actataacgg tcctaaggta gcgaaaatgt agtcttatgc
6961 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
7021 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
7081 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
7141 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
7201 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
7261 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
7321 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
7381 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
7441 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
7501 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
7561 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
7621 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
7681 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
7741 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
7801 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
7861 caagcccgct gatcttcaga cctggaggag gagatatgag ggacattgga gaagtgaatt
7921 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
7981 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
8041 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
8101 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
8161 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
8221 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
8281 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
8341 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
8401 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
8461 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
8521 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
8581 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
8641 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
8701 cagatccatt cgattagtga acggatctcg acggtatcga aagcttggga ttcgaattta
8761 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
8821 cagacataca aactaaagaa ctacaaaaac aaattacaaa aattcaaaat tttcgggttt
8881 ttcgaaccta gggttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
8941 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
9001 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
9061 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
9121 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
9181 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
9241 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
9301 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaac
LOCUS dna 9339 bp
FEATURES Location/Qualifiers
Promoter 6..87
/gene="CMV prom"
Regulatory_Seq 143..182
/gene="tetO reg"
Other Gene 196..320
/gene="attR1 other"
Other Gene 220..320
/gene="attR2 other"
misc_binding 321..328
/dbxref="REBASE:NotI"
Marker 429..1088
/gene="CAT marker"
ORF 429..1088
/sequence="ORF_4 rf(3)"
misc_binding 642..647
/dbxref="REBASE:EcoRI"
Other Gene 1430..1735
/gene="ccdB other"
misc_binding 1511..1516
/dbxref="REBASE:SmaI"
misc_binding 1511..1516
/dbxref="REBASE:XmaI"
misc_binding 1764..1769
/dbxref="REBASE:PstI"
Other Gene 1776..1895
/gene="attR1 other"
Other Gene 1776..1876
/gene="attR2 other"
misc_binding 1911..1916
/dbxref="REBASE:XhoI"
Promoter 1961..2229
/gene="SV40 prom"
Rep_Origin 2128..2205
/gene="SV40 origin"
ORF 2195..2863
/sequence="ORF_1 rf(2)"
misc_binding 2208..2220
/dbxref="REBASE:SfiI"
Marker 2264..2863
/gene="puro marker"
Other Gene 3473..3525
/gene="delta_U3 other"
Other Gene 3526..3706
/gene="HIV-1_5_LTR other"
Rep_Origin 4213..4832
/gene="pBR322 origin"
Promoter 5090..5139
/gene="NEOKAN prom"
misc_binding 5197..5202
/dbxref="REBASE:BclI"
ORF 5228..6022
/sequence="ORF_2 rf(2)"
Marker 5231..6019
/gene="NTP_II marker"
Rep_Origin 6127..6433
/gene="f1 origin"
Other Gene 6488..6589
/gene="RITR other"
Other Gene 6488..6589
/gene="LITR other"
Other Gene 6669..6817
/gene="Encap other"
Other Gene 7175..7355
/gene="HIV-1_5_LTR other"
Other Gene 7466..7510
/gene="HIV-1_psi_pack other"
misc_binding 7552..7557
/dbxref="REBASE:NruI"
Regulatory_Seq 8017..8250
/gene="RRE reg"
ORF 8060..8761
/sequence="ORF_3 rf(2)"
misc_binding 9071..9076
/dbxref="REBASE:NdeI"
BASE COUNT 2400 a 2219 c 2485 g 2235 t 0 others
ORIGIN
1 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
61 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
121 catagaagaa ccgagtttaa actccctatc agtgatagag atctccctat cagtgataga
181 gagctagcga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
241 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
301 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
361 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
421 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
481 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
541 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
601 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
661 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
721 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
781 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
841 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
901 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
961 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
1021 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
1081 gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
1141 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
1201 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
1261 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
1321 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
1381 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
1441 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
1501 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
1561 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
1621 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
1681 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
1741 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
1801 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
1861 cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatatctga ctcgagtacc
1921 catacgacgt cccagactac gcttgagttt aaacacgcgt ggtgtggaaa gtccccaggc
1981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
2041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
2101 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
2161 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
2221 tctgagctat tccagaagta gtgaggaggc ttttttggag gccatgaccg agtacaagcc
2281 cacggtgcgc ctcgccaccc gcgacgacgt ccctcgggcc gtacgcaccc tcgccgccgc
2341 gttcgccgac taccccgcca cgcgccacac cgtggacccg gaccgccaca tcgagcgggt
2401 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
2461 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
2521 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
2581 gcaacagatg gaagggctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
2641 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
2701 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
2761 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
2821 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt ccggaaatca
2881 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
2941 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
3001 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
3061 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
3121 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
3181 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
3241 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
3301 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
3361 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctcgcctt
3421 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtccg gatggaaggg
3481 ctaattcact cccaacgaat acaagatctg ctttttgctt gtactgggtc tctctggtta
3541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
3601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
3661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
3721 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
3781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
3841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
3901 catgtctggc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt
3961 atgggtctgc attaatgaat cggccaacgg atgtcggtgt gaaataccgc acagatgcgt
4021 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
4681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
4741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4861 cgaaaactca cgttaaggga ttttggccat gagattatca aaaaggatct tcacctagat
4921 ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
4981 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
5041 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
5101 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
5161 gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc …
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
CMV prom(6,87)>>>
|
1 aactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagc 60
TTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCG
61 agagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctc 120
TCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAG
tetO reg(143,182)>>>
|
121 catagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgataga 180
GTATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCT
attR1 other(196,320)>>> attR2 other(220,320)<<<
| |
181 gagctagcgatatcaacaagtttgtacaaaaaagctgaacgagaaacgtaaaatgatata 240
CTCGATCGCTATAGTTGTTCAAACATGTTTTTTCGACTTGCTCTTTGCATTTTACTATAT
241 aatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaaca 300
TTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTTGT
NotI
|
301 caacatatccagtcactatggcggccgcattaggcaccccaggctttacactttatgctt 360
GTTGTATAGGTCAGTGATACCGCCGGCGTAATCCGTGGGGTCCGAAATGTGAAATACGAA
361 ccggctcgtataatgtgtggattttgagttaggatccgtcgagattttcaggagctaagg 420
GGCCGAGCATATTACACACCTAAAACTCAATCCTAGGCAGCTCTAAAAGTCCTCGATTCC
ORF_4 rf(3)(429,1088)>>>
|
CAT marker(429,1088)>>>
|
421 aagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatc 480
TTCGATTTTACCTCTTTTTTTAGTGACCTATATGGTGGCAACTATATAGGGTTACCGTAG
481 gtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttc 540
CATTTCTTGTAAAACTCCGTAAAGTCAGTCAACGAGTTACATGGATATTGGTCTGGCAAG
541 agctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccgg 600
TCGACCTATAATGCCGGAAAAATTTCTGGCATTTCTTTTTATTCGTGTTCAAAATAGGCC
EcoRI
|
601 cctttattcacattcttgcccgcctgatgaatgctcatccggaattccgtatggcaatga 660
GGAAATAAGTGTAAGAACGGGCGGACTACTTACGAGTAGGCCTTAAGGCATACCGTTACT
661 aagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagc 720
TTCTGCCACTCGACCACTATACCCTATCACAAGTGGGAACAATGTGGCAAAAGGTACTCG
721 aaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctac 780
TTTGACTTTGCAAAAGTAGCGAGACCTCACTTATGGTGCTGCTAAAGGCCGTCAAAGATG
781 acatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggt 840
TGTATATAAGCGTTCTACACCGCACAATGCCACTTTTGGACCGGATAAAGGGATTTCCCA
841 ttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatt 900
AATAACTCTTATACAAAAAGCAGAGTCGGTTAGGGACCCACTCAAAGTGGTCAAAACTAA
901 taaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattata 960
ATTTGCACCGGTTATACCTGTTGAAGAAGCGGGGGCAAAAGTGGTACCCGTTTATAATAT
961 cgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatg 1020
GCGTTCCGCTGTTCCACGACTACGGCGACCGCTAAGTCCAAGTAGTACGGCAAACACTAC
1021 gcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcg 1080
CGAAGGTACAGCCGTCTTACGAATTACTTAATGTTGTCATGACGCTACTCACCGTCCCGC
1081 gggcgtaaagatctggatccggcttactaaaagccagataacagtatgcgtatttgcgcg 1140
CCCGCATTTCTAGACCTAGGCCGAATGATTTTCGGTCTATTGTCATACGCATAAACGCGC
1141 ctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagag 1200
GACTAAAAACGCCATATTCTTATATATGACTATACATATGGGCTTCATACAGTTTTTCTC
1201 gtatgctatgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaa 1260
CATACGATACTTCGTCGCATAATGTCACTGTCAACTGTCGCTGTCGATAGTCAACGAGTT
1261 ggcatatatgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgt 1320
CCGTATATACTACAGTTATAGAGGCCAGACCATTCGTGTTGGTACGTCTTACTTCGGGCA
1321 cgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccgg 1380
GCAGACGCACGGCTTGCGACCTTTCGCCTTTTAGTCCTTCCCTACCGACTCCAGCGGGCC
ccdB other(1430,1735)>>>
|
1381 tttattgaaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaa 1440
AAATAACTTTACTTGCCGAGAAAACGACTGCTCTTGTCCCCGACCACTTTACGTCAAATT
1441 ggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatat 1500
CCAAATGTGGATATTTTCTCTCTCGGCAATAGCAGACAAACACCTACATGTCTCACTATA
SmaI
|
XmaI
| |
1501 tattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcaga 1560
ATAACTGTGCGGGCCCGCTGCCTACCACTAGGGGGACCGGTCACGTGCAGACGACAGTCT
1561 taaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgat 1620
ATTTCAGAGGGCACTTGAAATGGGCCACCACGTATAGCCCCTACTTTCGACCGCGTACTA
1621 gaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcag 1680
CTGGTGGCTATACCGGTCACACGGCCAGAGGCAATAGCCCCTTCTTCACCGACTAGAGTC
1681 ccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtc 1740
GGTGGCGCTTTTACTGTAGTTTTTGCGGTAATTGGACTACAAGACCCCTTATATTTACAG
attR2 other(1776,1876)>>>
|
PstI attR1 other(1776,1895)<<<
| |
1741 aggctcccttatacacagccagtctgcaggtcgaccatagtgactggatatgttgtgttt 1800
TCCGAGGGAATATGTGTCGGTCAGACGTCCAGCTGGTATCACTGACCTATACAACACAAA
1801 tacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatat 1860
ATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATATA
XhoI
|
1861 cattttacgtttctcgttcagctttcttgtacaaagtggttgatatctgactcgagtacc 1920
GTAAAATGCAAAGAGCAAGTCGAAAGAACATGTTTCACCAACTATAGACTGAGCTCATGG
SV40 prom(1961,2229)>>>
|
1921 catacgacgtcccagactacgcttgagtttaaacacgcgtggtgtggaaagtccccaggc 1980
GTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCG
1981 tccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtgga 2040
AGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCT
2041 aagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagca 2100
TTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGT
SV40 origin(2128,2205)>>>
|
2101 accatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccat 2160
TGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTA
SfiI
|
ORF_1 rf(2)(2195,2863)>>>
| |
2161 tctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcc 2220
AGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGG
puro marker(2264,2863)>>>
|
2221 tctgagctattccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcc 2280
AGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGG
2281 cacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgc 2340
GTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCG
2341 gttcgccgactaccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggt 2400
CAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCA
2401 caccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggt 2460
GTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCA
2461 cgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggc 2520
GCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCG
2521 ggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgca 2580
CCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGT
2581 gcaacagatggaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggc 2640
CGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCG
2641 caccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccc 2700
GTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGG
2701 cggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccg 2760
GCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGC
2761 caacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccga 2820
GTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCT
2821 aggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatca 2880
TCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGT
2881 acctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttt 2940
TGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAA
2941 tacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggc 3000
ATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCG
3001 tttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcc 3060
AAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGG
3061 cgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttg 3120
GCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAAC
3121 gggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgc 3180
CCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACG
3181 cacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttggg 3240
GTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCC
3241 cactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctg 3300
GTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGAC
3301 tgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatcc 3360
ACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGG
3361 agcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgcctt 3420
TCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAA
delta_U3 other(3473,3525)>>>
|
3421 cgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaaggg 3480
GCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCC
HIV-1…
LOCUS dna 9339 bp
FEATURES Location/Qualifiers
Promoter 6..87
/gene="CMV prom"
Regulatory_Seq 143..182
/gene="tetO reg"
Other Gene 196..320
/gene="attR1 other"
Other Gene 220..320
/gene="attR2 other"
misc_binding 321..328
/dbxref="REBASE:NotI"
Marker 429..1088
/gene="CAT marker"
ORF 429..1088
/sequence="ORF_4 rf(3)"
misc_binding 642..647
/dbxref="REBASE:EcoRI"
Other Gene 1430..1735
/gene="ccdB other"
misc_binding 1511..1516
/dbxref="REBASE:SmaI"
misc_binding 1511..1516
/dbxref="REBASE:XmaI"
misc_binding 1764..1769
/dbxref="REBASE:PstI"
Other Gene 1776..1895
/gene="attR1 other"
Other Gene 1776..1876
/gene="attR2 other"
misc_binding 1911..1916
/dbxref="REBASE:XhoI"
Promoter 1961..2229
/gene="SV40 prom"
Rep_Origin 2128..2205
/gene="SV40 origin"
ORF 2195..2863
/sequence="ORF_1 rf(2)"
misc_binding 2208..2220
/dbxref="REBASE:SfiI"
Marker 2264..2863
/gene="puro marker"
Other Gene 3473..3525
/gene="delta_U3 other"
Other Gene 3526..3706
/gene="HIV-1_5_LTR other"
Rep_Origin 4213..4832
/gene="pBR322 origin"
Promoter 5090..5139
/gene="NEOKAN prom"
misc_binding 5197..5202
/dbxref="REBASE:BclI"
ORF 5228..6022
/sequence="ORF_2 rf(2)"
Marker 5231..6019
/gene="NTP_II marker"
Rep_Origin 6127..6433
/gene="f1 origin"
Other Gene 6488..6589
/gene="RITR other"
Other Gene 6488..6589
/gene="LITR other"
Other Gene 6669..6817
/gene="Encap other"
Other Gene 7175..7355
/gene="HIV-1_5_LTR other"
Other Gene 7466..7510
/gene="HIV-1_psi_pack other"
misc_binding 7552..7557
/dbxref="REBASE:NruI"
Regulatory_Seq 8017..8250
/gene="RRE reg"
ORF 8060..8761
/sequence="ORF_3 rf(2)"
misc_binding 9071..9076
/dbxref="REBASE:NdeI"
BASE COUNT 2400 a 2219 c 2485 g 2235 t 0 others
