pLenti-HOXA10-GFP
| Vector Name | pLenti-HOXA10-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc
3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact
3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg
3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg
3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc
3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc
3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg
3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac
3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct
3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac
3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg
3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca
3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc
3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc
3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg
3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg
4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag
4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga
4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag
4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca
4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta
4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgaggatcc tctagaggtg
4381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
4441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
4501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
4561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
4621 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
4681 gtcgaggcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
4741 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
4801 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
4861 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
4921 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
4981 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
5041 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
5101 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
5161 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
5221 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
5281 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
5341 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
5401 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
5461 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
5521 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
5581 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
5641 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
5701 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
5761 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
5821 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
5881 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
5941 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
6001 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
6061 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa
6121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
6181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
6241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
6301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
6361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
6421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
6481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
6541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
6601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc
6661 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc
6721 taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag
6781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
6841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
6901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
6961 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
7021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
7081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
7141 atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta
7201 tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa
7261 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
7321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
7381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
7441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
7501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
7561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
7621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
7681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
7741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
7801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
7861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
7921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
7981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
8041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
8101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
8161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
8221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
8281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
8341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
8401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
8461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
8521 gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca
8581 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
8641 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
8701 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
8761 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg
8821 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
8881 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
8941 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
9001 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
9061 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
9121 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
9181 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
9241 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
9301 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
9361 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
9421 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
9481 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
9541 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
9601 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt
9661 gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc
9721 cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga
9781 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
9841 atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag
9901 ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
9961 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
10021 tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc
10081 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 10139 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4364
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 4183..4188
/dbxref="REBASE:XhoI"
misc_binding 4371..4376
/dbxref="REBASE:XbaI"
Promoter 4377..4645
/gene="SV40 prom"
Rep_Origin 4544..4621
/gene="SV40 origin"
ORF 4693..6102
/sequence="ORF_2 rf(1)"
Marker 5503..6102
/gene="puro marker"
Other Gene 6712..6764
/gene="delta_U3 other"
Other Gene 6765..6945
/gene="HIV-1_5_LTR other"
Rep_Origin 7451..8070
/gene="pBR322 origin"
Promoter 8723..8772
/gene="NEOKAN prom"
misc_binding 8830..8835
/dbxref="REBASE:BclI"
ORF 8861..9655
/sequence="ORF_3 rf(2)"
Marker 8864..9652
/gene="NTP_II marker"
Rep_Origin 9760..10065
/gene="f1 origin"
BASE COUNT 2431 a 2703 c 2822 g 2183 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc
3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact
3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg
3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg
3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc
3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc
3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg
3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac
3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct
3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac
3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg
3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca
3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc
3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc
3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg
3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg
4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag
4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga
4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag
4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca
4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta
4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgaggatcc tctagaggtg
4381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
4441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
4501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
4561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
4621 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
4681 gtcgaggcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
4741 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
4801 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
4861 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
4921 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
4981 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
5041 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
5101 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
5161 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
5221 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
5281 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
5341 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
5401 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
5461 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
5521 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
5581 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
5641 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
5701 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
5761 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
5821 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
5881 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
5941 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
6001 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
6061 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa
6121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
6181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
6241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
6301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
6361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
6421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
6481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
6541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
6601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc
6661 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc
6721 taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag
6781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
6841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
6901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
6961 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
7021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
7081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
7141 atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta
7201 tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa
7261 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
7321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
7381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
7441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
7501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
7561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
7621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
7681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
7741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
7801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
7861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
7921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
7981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
8041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
8101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
8161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
8221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
8281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
8341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
8401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
8461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
8521 gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca
8581 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
8641 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
8701 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
8761 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg
8821 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
8881 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
8941 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
9001 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
9061 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
9121 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
9181 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
9241 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
9301 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
9361 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
9421 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
9481 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
9541 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
9601 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt
9661 gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc
9721 cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga
9781 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
9841 atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag
9901 ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
9961 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
10021 tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc
10081 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_4 rf(3)(2901,4364)>>>
|
CMV prom(2885,2966)>>>
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGTCAGCCAGAAAGGGCTATC 3120
TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACAGTCGGTCTTTCCCGATAG
3121 TGCTCCCTTCGCCAAATTATCCCACAACAATGTCATGCTCGGAGAGCCCCGCCGCGAACT 3180
ACGAGGGAAGCGGTTTAATAGGGTGTTGTTACAGTACGAGCCTCTCGGGGCGGCGCTTGA
3181 CTTTTTTGGTCGACTCGCTCATCAGCTCGGGCAGAGGCGAGGCAGGCGGCGGTGGTGGTG 3240
GAAAAAACCAGCTGAGCGAGTAGTCGAGCCCGTCTCCGCTCCGTCCGCCGCCACCACCAC
3241 GCGCGGGGGGCGGCGGCGGTGGCGGTTACTACGCCCACGGCGGGGTCTACCTGCCGCCCG 3300
CGCGCCCCCCGCCGCCGCCACCGCCAATGATGCGGGTGCCGCCCCAGATGGACGGCGGGC
3301 CCGCCGACCTGCCCTACGGGCTGCAGAGCTGCGGGCTCTTCCCCACGCTGGGCGGCAAGC 3360
GGCGGCTGGACGGGATGCCCGACGTCTCGACGCCCGAGAAGGGGTGCGACCCGCCGTTCG
3361 GCAATGAGGCAGCGTCGCCGGGCAGCGGTGGCGGTGGCGGGGGTCTAGGTCCCGGGGCGC 3420
CGTTACTCCGTCGCAGCGGCCCGTCGCCACCGCCACCGCCCCCAGATCCAGGGCCCCGCG
3421 ACGGCTACGGGCCCTCGCCCATAGACCTGTGGCTAGACGCGCCCCGGTCTTGCCGGATGG 3480
TGCCGATGCCCGGGAGCGGGTATCTGGACACCGATCTGCGCGGGGCCAGAACGGCCTACC
3481 AGCCGCCTGACGGGCCGCCGCCGCCGCCCCAGCAGCAGCCGCCGCCCCCGCCGCAACCAC 3540
TCGGCGGACTGCCCGGCGGCGGCGGCGGGGTCGTCGTCGGCGGCGGGGGCGGCGTTGGTG
3541 CCCAGCCAGCGCCGCAGGCCACCTCGTGCTCTTTCGCGCAGAACATCAAAGAAGAGAGCT 3600
GGGTCGGTCGCGGCGTCCGGTGGAGCACGAGAAAGCGCGTCTTGTAGTTTCTTCTCTCGA
3601 CCTACTGCCTCTACGACTCGGCGGACAAATGCCCCAAAGTCTCGGCCACCGCCGCCGAAC 3660
GGATGACGGAGATGCTGAGCCGCCTGTTTACGGGGTTTCAGAGCCGGTGGCGGCGGCTTG
3661 TGGCTCCCTTCCCGCGGGGCCCGCCGCCCGACGGCTGCGCCCTGGGCACCTCCAGCGGGG 3720
ACCGAGGGAAGGGCGCCCCGGGCGGCGGGCTGCCGACGCGGGACCCGTGGAGGTCGCCCC
3721 TGCCAGTGCCTGGCTACTTCCGCCTTTCTCAGGCCTACGGCACCGCCAAGGGCTATGGCA 3780
ACGGTCACGGACCGATGAAGGCGGAAAGAGTCCGGATGCCGTGGCGGTTCCCGATACCGT
3781 GCGGCGGCGGCGGCGCGCAGCAACTCGGGGCTGGCCCGTTCCCCGCGCAGCCCCCGGGGC 3840
CGCCGCCGCCGCCGCGCGTCGTTGAGCCCCGACCGGGCAAGGGGCGCGTCGGGGGCCCCG
3841 GCGGTTTCGATCTCCCGCCCGCGCTAGCCTCCGGCTCGGCCGATGCGGCCCGGAAGGAGC 3900
CGCCAAAGCTAGAGGGCGGGCGCGATCGGAGGCCGAGCCGGCTACGCCGGGCCTTCCTCG
3901 GAGCCCTCGATTCGCCGCCGCCCCCCACGCTGGCTTGCGGCAGCGGCGGGGGCTCGCAGG 3960
CTCGGGAGCTAAGCGGCGGCGGGGGGTGCGACCGAACGCCGTCGCCGCCCCCGAGCGTCC
3961 GCGACGAGGAGGCGCACGCGTCGTCCTCGGCCGCGGAGGAGCTCTCCCCGGCCCCTTCCG 4020
CGCTGCTCCTCCGCGTGCGCAGCAGGAGCCGGCGCCTCCTCGAGAGGGGCCGGGGAAGGC
4021 AGAGCAGCAAAGCCTCGCCGGAGAAGGATTCCCTGGGCAATTCCAAAGGTGAAAACGCAG 4080
TCTCGTCGTTTCGGAGCGGCCTCTTCCTAAGGGACCCGTTAAGGTTTCCACTTTTGCGTC
4081 CCAACTGGCTCACGGCAAAGAGTGGTCGGAAGAAGCGCTGCCCCTACACGAAGCACCAGA 4140
GGTTGACCGAGTGCCGTTTCTCACCAGCCTTCTTCGCGACGGGGATGTGCTTCGTGGTCT
XhoI
|
4141 CACTGGAGCTGGAGAAGGAGTTTCTGTTCAATATGTACCTTACTCGAGAGCGGCGCCTAG 4200
GTGACCTCGACCTCTTCCTCAAAGACAAGTTATACATGGAATGAGCTCTCGCCGCGGATC
4201 AGATTAGCCGCAGCGTCCACCTCACGGACAGACAAGTGAAAATCTGGTTTCAGAACCGCA 4260
TCTAATCGGCGTCGCAGGTGGAGTGCCTGTCTGTTCACTTTTAGACCAAAGTCTTGGCGT
4261 GGATGAAACTGAAGAAAATGAATCGAGAAAACCGGATCCGGGAGCTCACAGCCAACTTTA 4320
CCTACTTTGACTTCTTTTACTTAGCTCTTTTGGCCTAGGCCCTCGAGTGTCGGTTGAAAT
XbaI SV40 prom(4377,4645)>>>
| |
4321 ATTTTTCCtgcccaactttcttgtacaaagtggttgatatctgaggatcctctagaggtg 4380
TAAAAAGGACGGGTTGAAAGAACATGTTTCACCAACTATAGACTCCTAGGAGATCTCCAC
4381 tggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtc 4440
ACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAG
4441 agcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgca 4500
TCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGT
SV40 origin(4544,4621)>>>
|
4501 tctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactcc 4560
AGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGG
4561 gcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggc 4620
CGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCG
4621 cgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcct 4680
GCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGA
ORF_2 rf(1)(4693,6102)>>>
|
4681 gtcgaggccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgc 4740
CAGCTCCGGTGGTACCTCTCGCTGCTCTCGCCGGACGGGCGGTACCTCTAGCTCACGGCG
4741 atcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcaccccc 4800
TAGTGGCCGTGGGACTTGCCGCACCTCAAGCTCGACCACCCGCCGCCTCTCCCGTGGGGG
4801 aagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccc 4860
TTCGTCCCGGCGTACTGGTTGTTCTACTTCTCGTGGTTTCCGCGGGACTGGAAGTCGGGG
4861 tacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggc 4920
ATGGACGACTCGGTGCACTACCCGATGCCGAAGATGGTGAAGCCGTGGATGGGGTCGCCG
4921 tacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgag 4980
ATGCTCTTGGGGAAGGACGTGCGGTAGTTGTTGCCGCCGATGTGGTTGTGGGCGTAGCTC
4981 aagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgc 5040
TTCATGCTCCTGCCGCCGCACGACGTGCACTCGAAGTCGATGGCGATGCTCCGGCCGGCG
5041 gtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcacc 5100
CACTAGCCGCTGAAGTTCCACCACCCGTGGCCGAAGGGGCTCCTGTCGCACTAGAAGTGG
5101 gacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtg 5160
CTGTTCTAGTAGGCGTCGTTGCGGTGGCACCTCGTGGACGTGGGGTACCCGCTATTGCAC
5161 ctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtg 5220
GACCACCCGTCGAAGCGGGCGTGGAAGTCGGACGCGCTGCCGCCGATGATGTCGAAGCAC
5221 gtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggc 5280
CACCTGTCGGTGTACGTGAAGTTCTCGCGGTAGGTGGGGTCGTAGGACGTCTTGCCCCCG
5281 cccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtg 5340
GGGTACAAGCGGAAGGCGGCGCACCTCCTCGACGTGTCGTTGTGGCTCGACCCGTAGCAC
5341 gagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtcc 5400
CTCATGGTCGTGCGGAAGTTCTGGGGGTAGCGGAAGCGGTCTAGGGCGCGAGTCAGCAGG
5401 aattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggcagagga 5460
TTAAGACGGCACCTGCCGTGGCGGCCTGGGCCGAGGTGGCCTAGAGCGCTCCCGTCTCCT
puro marker(5503,6102)>>>
|
5461 agtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtacaagccc 5520
TCAGAAGATTGTACGCCACTGCACCTCCTCTTAGGGCCGGGATACTGGCTCATGTTCGGG
5521 acggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcg 5580
TGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGC
5581 ttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtc 5640
AAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAG
5641 accgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtc 5700
TGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAG
5701 gcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcg 5760
CGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGC
5761 gtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcag 5820
CACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTC
5821 caacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggcc 5880
GTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGG
5881 accgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctcccc 5940
TGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGG
5941 ggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgc 6000
CCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCG
6001 aacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaa 6060
TTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTT
6061 ggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaa 6120
CCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTT
6121 cctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctcctttt 6180
GGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAA
6181 acgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggct 6240
TGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGA
6241 ttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggccc 6300
AAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGG
6301 gttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttgg 6360
CAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACC
6361 ggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgcc 6420
CCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGG
6421 acggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggc 6480
TGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCG
6481 actgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgt 6540
TGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACA
6541 gttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatcca 6600
CAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGT
6601 gcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttc 6660
CGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAG
delta_U3 other(6712,6764)>>>
|
6661 gccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggc 6720
CGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCG
HIV-1_5_LTR other(6765,6945)>>>
|
6721 taattcactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttag 6780
ATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATC
6781 accagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaat 6840
TGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTA
6841 aaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaact 6900
TTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGA
6901 agagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtc 6960
TCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAG
6961 atcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaact 7020
TAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGA
7021 tgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaata 7080
ACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTAT
7081 aagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatc 7140
TTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAG
7141 atgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtatta 7200
TACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAAT
7201 tgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaa 7260
ACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATT
7261 ggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcgg 7320
CCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCC
7321 tcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacag 7380
AGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTC
7381 aatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaacc 7440
TTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGG
pBR322 origin(7451,8070)<<<
|
7441 gtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcaca 7500
CATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGT
7501 aaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgt 7560
TTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCA
7561 ttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacc 7620
AAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGG
7621 tgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatc 7680
ACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAG
7681 tcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagc 7740
AGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCG
7741 ccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgact 7800
GGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGA
7801 tatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtg 7860
ATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCAC
7861 ctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggta 7920
GATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCAT
7921 tctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca 7980
AGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGT
7981 aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaa 8040
TTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTT
8041 aaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacg 8100
TTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGC
8101 aaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcc 8160
TTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGG
8161 ttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctg 8220
AAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGAC
8221 acagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcat 8280
TGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTA
8281 ccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctg 8340
GGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGAC
8341 gccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaa 8400
CGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTT
8401 taaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcca 8460
ATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGT
8461 tccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgc 8520
AGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACG
8521 gcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgca 8580
CGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGT
8581 gaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgc 8640
CTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCG
8641 aagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggc 8700
TTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCG
NEOKAN prom(8723,8772)>>>
|
8701 ggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgg 8760
CCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACC
8761 gaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcagggg 8820
CTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCC
NTP_II marker(8864,9652)>>>
|
BclI ORF_3 rf(2)(8861,9655)>>>
| | |
8821 atcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggatt 8880
TAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAA
8881 gcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaaca 8940
CGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGT
8941 gacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttct 9000
CTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGA
9001 ttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggct 9060
AAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGA
9061 atcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagc 9120
TAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCG
9121 gggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacct 9180
CCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGA
9181 tgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttga 9240
ACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACT
9241 tccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcg 9300
AGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGC
9301 gatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgcc 9360
CTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGG
9361 agccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgac 9420
TCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTG
9421 ccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcat 9480
GGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTA
9481 cgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtga 9540
GCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACT
9541 tattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgc 9600
ATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCG
9601 cgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaatttt 9660
GCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAA
9661 gttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcc 9720
CAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGG
f1 origin(9760,10065)<<<
|
9721 cttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaaga 9780
GAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCT
9781 gtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcg 9840
CAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGC
9841 atggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaag 9900
TACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTC
9901 ctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcga 9960
GAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCT
9961 acgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtg 10020
TGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCAC
10021 tagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgc 10080
ATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCG
10081 gtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 10139
CAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 10139 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4364
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 4183..4188
/dbxref="REBASE:XhoI"
misc_binding 4371..4376
/dbxref="REBASE:XbaI"
Promoter 4377..4645
/gene="SV40 prom"
Rep_Origin 4544..4621
/gene="SV40 origin"
ORF 4693..6102
/sequence="ORF_2 rf(1)"
Marker 5503..6102
/gene="puro marker"
Other Gene 6712..6764
/gene="delta_U3 other"
Other Gene 6765..6945
/gene="HIV-1_5_LTR other"
Rep_Origin 7451..8070
/gene="pBR322 origin"
Promoter 8723..8772
/gene="NEOKAN prom"
misc_binding 8830..8835
/dbxref="REBASE:BclI"
ORF 8861..9655
/sequence="ORF_3 rf(2)"
Marker 8864..9652
/gene="NTP_II marker"
Rep_Origin 9760..10065
/gene="f1 origin"
BASE COUNT 2431 a 2703 c 2822 g 2183 t 0 others