pLenti-HOXA10-RFP
| Vector Name | pLenti-HOXA10-RFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc
3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact
3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg
3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg
3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc
3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc
3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg
3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac
3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct
3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac
3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg
3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca
3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc
3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc
3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg
3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg
4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag
4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga
4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag
4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca
4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta
4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgactcgag tgaatgaatg
4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
4441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
4561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
4621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
4681 ggccactagt gtcgagaaaa ctagtcgcca ccatggtgag cgagctgatt aaggagaaca
4741 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
4801 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg
4861 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct
4921 tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca
4981 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
5041 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
5101 acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc
5161 ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc
5221 acctgatctg caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga
5281 tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga
5341 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
5401 ggcacagaga attcggaagc gggcagtgca ccaactacgc cctgctgaag ctggccggcg
5461 acgtggagag caaccccggc cccggatcca tggccaccga gtacaagccc acggtgcgcc
5521 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
5581 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
5641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
5701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
5761 agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
5821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
5881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
5941 cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
6001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
6061 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt
6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
6481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac
6661 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc
6721 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
7021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
7081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca
7141 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
7201 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata
7261 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
8521 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
8581 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
8641 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
8701 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
8761 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct
8821 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
8881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
8941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
9001 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
9061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
9121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
9181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
9241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
9301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
9361 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
9421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
9481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
9541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
9601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
9661 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc
9721 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
9781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
9841 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg
9901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
9961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
10021 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc
10081 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 10129 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4364
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3321..3326
/dbxref="REBASE:PstI"
Promoter 4382..4650
/gene="SV40 prom"
Rep_Origin 4549..4626
/gene="SV40 origin"
ORF 4713..6092
/sequence="ORF_4 rf(3)"
misc_binding 5409..5414
/dbxref="REBASE:EcoRI"
Marker 5496..6092
/gene="puro marker"
Other Gene 6702..6754
/gene="delta_U3 other"
Other Gene 6755..6935
/gene="HIV-1_5_LTR other"
Rep_Origin 7441..8060
/gene="pBR322 origin"
Promoter 8713..8762
/gene="NEOKAN prom"
misc_binding 8820..8825
/dbxref="REBASE:BclI"
ORF 8851..9645
/sequence="ORF_2 rf(1)"
Marker 8854..9642
/gene="NTP_II marker"
Rep_Origin 9750..10055
/gene="f1 origin"
BASE COUNT 2477 a 2665 c 2804 g 2183 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc
3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact
3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg
3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg
3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc
3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc
3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg
3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac
3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct
3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac
3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg
3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca
3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc
3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc
3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg
3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg
4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag
4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga
4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag
4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca
4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta
4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgactcgag tgaatgaatg
4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
4441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
4561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
4621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
4681 ggccactagt gtcgagaaaa ctagtcgcca ccatggtgag cgagctgatt aaggagaaca
4741 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
4801 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg
4861 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct
4921 tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca
4981 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
5041 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
5101 acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc
5161 ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc
5221 acctgatctg caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga
5281 tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga
5341 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
5401 ggcacagaga attcggaagc gggcagtgca ccaactacgc cctgctgaag ctggccggcg
5461 acgtggagag caaccccggc cccggatcca tggccaccga gtacaagccc acggtgcgcc
5521 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
5581 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
5641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
5701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
5761 agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
5821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
5881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
5941 cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
6001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
6061 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt
6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
6481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac
6661 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc
6721 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
7021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
7081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca
7141 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
7201 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata
7261 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
8521 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
8581 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
8641 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
8701 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
8761 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct
8821 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
8881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
8941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
9001 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
9061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
9121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
9181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
9241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
9301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
9361 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
9421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
9481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
9541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
9601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
9661 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc
9721 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
9781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
9841 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg
9901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
9961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
10021 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc
10081 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_3 rf(3)(2901,4364)>>>
|
CMV prom(2885,2966)>>>
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGTCAGCCAGAAAGGGCTATC 3120
TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACAGTCGGTCTTTCCCGATAG
3121 TGCTCCCTTCGCCAAATTATCCCACAACAATGTCATGCTCGGAGAGCCCCGCCGCGAACT 3180
ACGAGGGAAGCGGTTTAATAGGGTGTTGTTACAGTACGAGCCTCTCGGGGCGGCGCTTGA
3181 CTTTTTTGGTCGACTCGCTCATCAGCTCGGGCAGAGGCGAGGCAGGCGGCGGTGGTGGTG 3240
GAAAAAACCAGCTGAGCGAGTAGTCGAGCCCGTCTCCGCTCCGTCCGCCGCCACCACCAC
3241 GCGCGGGGGGCGGCGGCGGTGGCGGTTACTACGCCCACGGCGGGGTCTACCTGCCGCCCG 3300
CGCGCCCCCCGCCGCCGCCACCGCCAATGATGCGGGTGCCGCCCCAGATGGACGGCGGGC
PstI
|
3301 CCGCCGACCTGCCCTACGGGCTGCAGAGCTGCGGGCTCTTCCCCACGCTGGGCGGCAAGC 3360
GGCGGCTGGACGGGATGCCCGACGTCTCGACGCCCGAGAAGGGGTGCGACCCGCCGTTCG
3361 GCAATGAGGCAGCGTCGCCGGGCAGCGGTGGCGGTGGCGGGGGTCTAGGTCCCGGGGCGC 3420
CGTTACTCCGTCGCAGCGGCCCGTCGCCACCGCCACCGCCCCCAGATCCAGGGCCCCGCG
3421 ACGGCTACGGGCCCTCGCCCATAGACCTGTGGCTAGACGCGCCCCGGTCTTGCCGGATGG 3480
TGCCGATGCCCGGGAGCGGGTATCTGGACACCGATCTGCGCGGGGCCAGAACGGCCTACC
3481 AGCCGCCTGACGGGCCGCCGCCGCCGCCCCAGCAGCAGCCGCCGCCCCCGCCGCAACCAC 3540
TCGGCGGACTGCCCGGCGGCGGCGGCGGGGTCGTCGTCGGCGGCGGGGGCGGCGTTGGTG
3541 CCCAGCCAGCGCCGCAGGCCACCTCGTGCTCTTTCGCGCAGAACATCAAAGAAGAGAGCT 3600
GGGTCGGTCGCGGCGTCCGGTGGAGCACGAGAAAGCGCGTCTTGTAGTTTCTTCTCTCGA
3601 CCTACTGCCTCTACGACTCGGCGGACAAATGCCCCAAAGTCTCGGCCACCGCCGCCGAAC 3660
GGATGACGGAGATGCTGAGCCGCCTGTTTACGGGGTTTCAGAGCCGGTGGCGGCGGCTTG
3661 TGGCTCCCTTCCCGCGGGGCCCGCCGCCCGACGGCTGCGCCCTGGGCACCTCCAGCGGGG 3720
ACCGAGGGAAGGGCGCCCCGGGCGGCGGGCTGCCGACGCGGGACCCGTGGAGGTCGCCCC
3721 TGCCAGTGCCTGGCTACTTCCGCCTTTCTCAGGCCTACGGCACCGCCAAGGGCTATGGCA 3780
ACGGTCACGGACCGATGAAGGCGGAAAGAGTCCGGATGCCGTGGCGGTTCCCGATACCGT
3781 GCGGCGGCGGCGGCGCGCAGCAACTCGGGGCTGGCCCGTTCCCCGCGCAGCCCCCGGGGC 3840
CGCCGCCGCCGCCGCGCGTCGTTGAGCCCCGACCGGGCAAGGGGCGCGTCGGGGGCCCCG
3841 GCGGTTTCGATCTCCCGCCCGCGCTAGCCTCCGGCTCGGCCGATGCGGCCCGGAAGGAGC 3900
CGCCAAAGCTAGAGGGCGGGCGCGATCGGAGGCCGAGCCGGCTACGCCGGGCCTTCCTCG
3901 GAGCCCTCGATTCGCCGCCGCCCCCCACGCTGGCTTGCGGCAGCGGCGGGGGCTCGCAGG 3960
CTCGGGAGCTAAGCGGCGGCGGGGGGTGCGACCGAACGCCGTCGCCGCCCCCGAGCGTCC
3961 GCGACGAGGAGGCGCACGCGTCGTCCTCGGCCGCGGAGGAGCTCTCCCCGGCCCCTTCCG 4020
CGCTGCTCCTCCGCGTGCGCAGCAGGAGCCGGCGCCTCCTCGAGAGGGGCCGGGGAAGGC
4021 AGAGCAGCAAAGCCTCGCCGGAGAAGGATTCCCTGGGCAATTCCAAAGGTGAAAACGCAG 4080
TCTCGTCGTTTCGGAGCGGCCTCTTCCTAAGGGACCCGTTAAGGTTTCCACTTTTGCGTC
4081 CCAACTGGCTCACGGCAAAGAGTGGTCGGAAGAAGCGCTGCCCCTACACGAAGCACCAGA 4140
GGTTGACCGAGTGCCGTTTCTCACCAGCCTTCTTCGCGACGGGGATGTGCTTCGTGGTCT
4141 CACTGGAGCTGGAGAAGGAGTTTCTGTTCAATATGTACCTTACTCGAGAGCGGCGCCTAG 4200
GTGACCTCGACCTCTTCCTCAAAGACAAGTTATACATGGAATGAGCTCTCGCCGCGGATC
4201 AGATTAGCCGCAGCGTCCACCTCACGGACAGACAAGTGAAAATCTGGTTTCAGAACCGCA 4260
TCTAATCGGCGTCGCAGGTGGAGTGCCTGTCTGTTCACTTTTAGACCAAAGTCTTGGCGT
4261 GGATGAAACTGAAGAAAATGAATCGAGAAAACCGGATCCGGGAGCTCACAGCCAACTTTA 4320
CCTACTTTGACTTCTTTTACTTAGCTCTTTTGGCCTAGGCCCTCGAGTGTCGGTTGAAAT
4321 ATTTTTCCtgcccaactttcttgtacaaagtggttgatatctgactcgagtgaatgaatg 4380
TAAAAAGGACGGGTTGAAAGAACATGTTTCACCAACTATAGACTGAGCTCACTTACTTAC
SV40 prom(4382,4650)>>>
|
4381 aggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaat 4440
TCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTA
4441 tagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagc 4500
ATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCG
SV40 origin(4549,4626)>>>
|
4501 atgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgccccta 4560
TACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGAT
4561 actccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgca 4620
TGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGT
4621 gaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttgga 4680
CTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCT
ORF_4 rf(3)(4713,6092)>>>
|
4681 ggccactagtgtcgagaaaactagtcgccaccatggtgagcgagctgattaaggagaaca 4740
CCGGTGATCACAGCTCTTTTGATCAGCGGTGGTACCACTCGCTCGACTAATTCCTCTTGT
4741 tgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccg 4800
ACGTGTACTTCGACATGTACCTCCCGTGGCACTTGTTGGTGGTGAAGTTCACGTGTAGGC
4801 agggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcg 4860
TCCCGCTTCCGTTCGGGATGCTCCCGTGGGTCTGGTACTCTTAGTTCCGCCAGCTCCCGC
4861 gccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaacct 4920
CGGGAGAGGGGAAGCGGAAGCTGTAGGACCGATGGTCGAAGTACATGCCGTCGTTTTGGA
4921 tcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttca 4980
AGTAGTTGGTGTGGGTCCCGTAGGGGCTGAAGAAATTCGTCAGGAAGGGGCTCCCGAAGT
4981 catgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacacca 5040
GTACCCTCTCTCAGTGGTGTATGCTTCTGCCCCCGCACGACTGGCGATGGGTCCTGTGGT
5041 gcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatcca 5100
CGGAGGTCCTGCCGACGGAGTAGATGTTGCAGTTCTAGTCTCCCCACTTGAAGGGTAGGT
5101 acggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtacc 5160
TGCCGGGACACTACGTCTTCTTTTGTGAGCCGACCCTCCGGAGGTGGCTCTGGGACATGG
5161 ccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggcc 5220
GGCGACTGCCGCCGGACCTTCCGTCTCGGCTGTACCGGGACTTCGAGCACCCGCCCCCGG
5221 acctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaaga 5280
TGGACTAGACGTTGAACTTCTGGTGTATGTCTAGGTTCTTTGGGCGATTCTTGGAGTTCT
5281 tgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagaga 5340
ACGGGCCGCAGATGATACACCTGTCTTCTGACCTTTCTTAGTTCCTCCGGCTGTTTCTCT
5341 cctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactgg 5400
GGATGCAGCTCGTCGTGCTCCACCGACACCGGTCTATGACGCTGGAGGGATCGTTTGACC
EcoRI
|
5401 ggcacagagaattcggaagcgggcagtgcaccaactacgccctgctgaagctggccggcg 5460
CCGTGTCTCTTAAGCCTTCGCCCGTCACGTGGTTGATGCGGGACGACTTCGACCGGCCGC
puro marker(5496,6092)>>>
|
5461 acgtggagagcaaccccggccccggatccatggccaccgagtacaagcccacggtgcgcc 5520
TGCACCTCTCGTTGGGGCCGGGGCCTAGGTACCGGTGGCTCATGTTCGGGTGCCACGCGG
5521 tcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgact 5580
AGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGA
5581 accccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgc 5640
TGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACG
5641 aagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacg 5700
TTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGC
5701 gcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccg 5760
CGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGC
5761 agatcggctcgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatgg 5820
TCTAGCCGAGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACC
5821 aaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcg 5880
TTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGC
5881 tctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggagg 5940
AGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCC
5941 cggccgagcgcgctggggtgcccgccttcctggagacctccgcgccccgcaacctcccct 6000
GCCGGCTCGCGCGACCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGA
6001 tctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgca 6060
AGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGT
6061 cctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggatt 6120
GGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAA
6121 acaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtg 6180
TGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACAC
6181 gatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttct 6240
CTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGA
6241 cctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggc 6300
GGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCG
6301 aacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgcca 6360
TTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGT
6361 ccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaac 6420
GGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTG
6421 tcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaatt 6480
AGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAA
6481 ccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacct 6540
GGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGA
6541 ggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttc 6600
CCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAG
6601 cttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagac 6660
GAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTG
delta_U3 other(6702,6754)>>>
|
6661 gagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcactc 6720
CTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAG
HIV-1_5_LTR other(6755,6935)>>>
|
6721 ccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctg 6780
GGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGAC
6781 agcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgcc 6840
TCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGG
6841 ttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct 6900
AACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGA
6901 cagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattat 6960
GTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATA
6961 tcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgc 7020
AGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACG
7021 agcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttt 7080
TCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAA
7081 ttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggca 7140
AAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGT
7141 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 7200
AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT
7201 ttaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaata 7260
AATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTAT
7261 ccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggct 7320
GGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGA
7321 gcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggga 7380
CGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCT
7381 taacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggc 7440
ATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCG
pBR322 origin(7441,8060)<<<
|
7441 cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacg 7500
GCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGC
7501 ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg 7560
GAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACC
7561 aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctt 7620
TTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAA
7621 tctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggt 7680
AGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCA
7681 gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg 7740
CATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGAC
7741 cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccact 7800
GCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGA
7801 ggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagtt 7860
CCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAA
7861 cttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctct 7920
GAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGA
7921 gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccac 7980
CGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTG
7981 cgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatc 8040
GCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAG
8041 tcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacg 8100
AGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGC
8101 ttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta 8160
AATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAAT
8161 aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacca 8220
TTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGT
8221 atgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgc 8280
TACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACG
8281 ctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgc 8340
GACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACG
8341 tgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc 8400
ACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGG
8401 agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctat 8460
TCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATA
8461 taattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgt 8520
ATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACA
8521 tgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgc 8580
ACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACG
8581 tgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaag 8640
ACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTC
8641 agaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatgg 8700
TCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACC
NEOKAN prom(8713,8762)>>>
|
8701 acagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgc 8760
TGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACG
8761 aaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctct 8820
TTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGA
NTP_II marker(8854,9642)>>>
|
BclI ORF_2 rf(1)(8851,9645)>>>
| | |
8821 gatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggt 8880
CTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCA
8881 tctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggc 8940
AGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCG
8941 tgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaag 9000
ACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTC
9001 accgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctg 9060
TGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGAC
9061 gccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggac 9120
CGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTG
9121 tggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgcc 9180
ACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGG
9181 gagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacc 9240
CTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGG
9241 tgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagcc 9300
ACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGG
9301 ggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactg 9360
CCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGAC
9361 ttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgat 9420
AAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTA
9421 gcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggc 9480
CGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCG
9481 cggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaa 9540
GCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTT
9541 gagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgat 9600
CTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTA
9601 tcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaattt 9660
AGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAA
9661 ttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatc 9720
AACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAG
f1 origin(9750,10055)<<<
|
9721 aaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactatt 9780
TTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAA
9781 aaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccact 9840
TTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGA
9841 acgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcg 9900
TGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGC
9901 gaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgag 9960
CTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTC
9961 aaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcac 10020
TTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTG
10021 gctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgc 10080
CGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCG
10081 cattcaggatcgaattaattcttaattaacatcatcaataatatacctt 10129
GTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 10129 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..4364
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3321..3326
/dbxref="REBASE:PstI"
Promoter 4382..4650
/gene="SV40 prom"
Rep_Origin 4549..4626
/gene="SV40 origin"
ORF 4713..6092
/sequence="ORF_4 rf(3)"
misc_binding 5409..5414
/dbxref="REBASE:EcoRI"
Marker 5496..6092
/gene="puro marker"
Other Gene 6702..6754
/gene="delta_U3 other"
Other Gene 6755..6935
/gene="HIV-1_5_LTR other"
Rep_Origin 7441..8060
/gene="pBR322 origin"
Promoter 8713..8762
/gene="NEOKAN prom"
misc_binding 8820..8825
/dbxref="REBASE:BclI"
ORF 8851..9645
/sequence="ORF_2 rf(1)"
Marker 8854..9642
/gene="NTP_II marker"
Rep_Origin 9750..10055
/gene="f1 origin"
BASE COUNT 2477 a 2665 c 2804 g 2183 t 0 others