pLenti-HOXA9-C-term-HA
| Vector Name | pLenti-HOXA9-C-term-HA |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat ggccaccact ggggccctgg
3121 gcaactacta cgtggactcg ttcctgctgg gcgccgacgc cgcggatgag ctgagcgttg
3181 gccgctatgc gccggggacc ctgggccagc ctccccggca ggcggcgacg ctggccgagc
3241 accccgactt cagcccgtgc agcttccagt ccaaggcgac ggtgtttggc gcctcgtgga
3301 acccagtgca cgcggcgggc gccaacgctg tacccgctgc ggtgtaccac caccatcacc
3361 accaccccta cgtgcacccc caggcgcccg tggcggcggc ggcgccggac ggcaggtaca
3421 tgcgctcctg gctggagccc acgcccggtg cgctctcctt cgcgggcttg ccctccagcc
3481 ggccttatgg cattaaacct gaaccgctgt cggccagaag gggtgactgt cccacgcttg
3541 acactcacac tttgtccctg actgactatg cttgtggttc tcctccagtt gatagagaaa
3601 aacaacccag cgaaggcgcc ttctctgaaa acaatgctga gaatgagagc ggcggagaca
3661 agccccccat cgatcccaat aacccagcag ccaactggct tcatgcgcgc tccactcgga
3721 aaaagcggtg cccctataca aaacaccaga ccctggaact ggagaaagag tttctgttca
3781 acatgtacct caccagggac cgcaggtacg aggtggctcg actgctcaac ctcaccgaga
3841 ggcaggtcaa gatctggttc cagaaccgca ggatgaaaat gaagaaaatc aacaaagacc
3901 gagcaaaaga cgagtgccca actttcttgt acaaagtggt tgatatcctc gagtacccat
3961 acgacgtccc agactacgct tgagtttaaa cacgcgtggt gtggaaagtc cccaggctcc
4021 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
4081 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
4141 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
4201 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
4261 gagctattcc agaagtagtg aggaggcttt tttggaggcc atgaccgagt acaagcccac
4321 ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta cgcaccctcg ccgccgcgtt
4381 cgccgactac cccgccacgc gccacaccgt ggacccggac cgccacatcg agcgggtcac
4441 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
4501 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
4561 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
4621 acagatggaa gggctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
4681 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
4741 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
4801 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
4861 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg gaaatcaacc
4921 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
4981 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
5041 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
5101 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
5161 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
5221 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
5281 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
5341 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
5401 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tcgccttcgc
5461 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat ggaagggcta
5521 attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct ctggttagac
5581 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
5641 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
5701 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
5761 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
5821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
5881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
5941 gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat gggtattatg
6001 ggtctgcatt aatgaatcgg ccaacgatcc cggtgtgaaa taccgcacag atgcgtaagg
6061 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
6121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
6181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
6241 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
6301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
6361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
6421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
6481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
6541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
6601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
6661 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
6721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
6781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
6841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
6901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
6961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
7021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
7081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
7141 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
7201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
7261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
7321 aacgttgttg aaaaaggatc ttcacctaga tccttttcac gtagaaagcc agtccgcaga
7381 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
7441 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
7501 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
7561 agccctgcaa agtaaactgg atggctttct cgccgccaag gatctgatgg cgcaggggat
7621 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
7681 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
7741 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
7801 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
7861 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
7921 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
7981 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
8041 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
8101 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
8161 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
8221 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
8281 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
8341 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
8401 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattttgt
8461 taaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caacatccct
8521 tataaatcaa aagaatagac cgcgataggg ttgagtgttg ttccagtttg gaacaagagt
8581 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
8641 ggcccactac gtgaaccatc acccaaatca agttttttgc ggtcgaggtg ccgtaaagct
8701 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
8761 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
8821 gcggtcacgc tgcgcgtaac caccacaccc gcgcgcttaa tgcgccgcta cagggcgcgt
8881 ccattcgcca ttcaggatcg aattaattct taattaacat catcaataat atacctt
//
LOCUS dna 8937 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3983
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 3072..4046
/sequence="ORF_5 rf(5)"
misc_binding 3112..3117
/dbxref="REBASE:ApaI"
misc_binding 3669..3674
/dbxref="REBASE:ClaI"
misc_binding 3948..3953
/dbxref="REBASE:XhoI"
Promoter 3998..4266
/gene="SV40 prom"
Rep_Origin 4165..4242
/gene="SV40 origin"
ORF 4232..4900
/sequence="ORF_2 rf(2)"
misc_binding 4245..4257
/dbxref="REBASE:SfiI"
Marker 4301..4900
/gene="puro marker"
Other Gene 5510..5562
/gene="delta_U3 other"
Other Gene 5563..5743
/gene="HIV-1_5_LTR other"
Rep_Origin 6249..6868
/gene="pBR322 origin"
Promoter 7521..7570
/gene="NEOKAN prom"
misc_binding 7628..7633
/dbxref="REBASE:BclI"
ORF 7659..8453
/sequence="ORF_4 rf(3)"
Marker 7662..8450
/gene="NTP_II marker"
Rep_Origin 8558..8863
/gene="f1 origin"
BASE COUNT 2232 a 2264 c 2418 g 2023 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat ggccaccact ggggccctgg
3121 gcaactacta cgtggactcg ttcctgctgg gcgccgacgc cgcggatgag ctgagcgttg
3181 gccgctatgc gccggggacc ctgggccagc ctccccggca ggcggcgacg ctggccgagc
3241 accccgactt cagcccgtgc agcttccagt ccaaggcgac ggtgtttggc gcctcgtgga
3301 acccagtgca cgcggcgggc gccaacgctg tacccgctgc ggtgtaccac caccatcacc
3361 accaccccta cgtgcacccc caggcgcccg tggcggcggc ggcgccggac ggcaggtaca
3421 tgcgctcctg gctggagccc acgcccggtg cgctctcctt cgcgggcttg ccctccagcc
3481 ggccttatgg cattaaacct gaaccgctgt cggccagaag gggtgactgt cccacgcttg
3541 acactcacac tttgtccctg actgactatg cttgtggttc tcctccagtt gatagagaaa
3601 aacaacccag cgaaggcgcc ttctctgaaa acaatgctga gaatgagagc ggcggagaca
3661 agccccccat cgatcccaat aacccagcag ccaactggct tcatgcgcgc tccactcgga
3721 aaaagcggtg cccctataca aaacaccaga ccctggaact ggagaaagag tttctgttca
3781 acatgtacct caccagggac cgcaggtacg aggtggctcg actgctcaac ctcaccgaga
3841 ggcaggtcaa gatctggttc cagaaccgca ggatgaaaat gaagaaaatc aacaaagacc
3901 gagcaaaaga cgagtgccca actttcttgt acaaagtggt tgatatcctc gagtacccat
3961 acgacgtccc agactacgct tgagtttaaa cacgcgtggt gtggaaagtc cccaggctcc
4021 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
4081 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
4141 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
4201 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
4261 gagctattcc agaagtagtg aggaggcttt tttggaggcc atgaccgagt acaagcccac
4321 ggtgcgcctc gccacccgcg acgacgtccc tcgggccgta cgcaccctcg ccgccgcgtt
4381 cgccgactac cccgccacgc gccacaccgt ggacccggac cgccacatcg agcgggtcac
4441 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
4501 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
4561 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
4621 acagatggaa gggctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
4681 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
4741 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
4801 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
4861 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acgcgttccg gaaatcaacc
4921 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
4981 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
5041 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
5101 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
5161 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
5221 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
5281 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
5341 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
5401 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tcgccttcgc
5461 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtccggat ggaagggcta
5521 attcactccc aacgaataca agatctgctt tttgcttgta ctgggtctct ctggttagac
5581 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
5641 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
5701 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
5761 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
5821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
5881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
5941 gtctggcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat gggtattatg
6001 ggtctgcatt aatgaatcgg ccaacgatcc cggtgtgaaa taccgcacag atgcgtaagg
6061 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
6121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
6181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
6241 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
6301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
6361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
6421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
6481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
6541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
6601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
6661 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
6721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
6781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
6841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
6901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
6961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
7021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
7081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
7141 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
7201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
7261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
7321 aacgttgttg aaaaaggatc ttcacctaga tccttttcac gtagaaagcc agtccgcaga
7381 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
7441 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
7501 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
7561 agccctgcaa agtaaactgg atggctttct cgccgccaag gatctgatgg cgcaggggat
7621 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
7681 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
7741 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
7801 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
7861 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
7921 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
7981 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
8041 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
8101 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
8161 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
8221 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
8281 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
8341 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
8401 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattttgt
8461 taaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caacatccct
8521 tataaatcaa aagaatagac cgcgataggg ttgagtgttg ttccagtttg gaacaagagt
8581 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
8641 ggcccactac gtgaaccatc acccaaatca agttttttgc ggtcgaggtg ccgtaaagct
8701 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
8761 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
8821 gcggtcacgc tgcgcgtaac caccacaccc gcgcgcttaa tgcgccgcta cagggcgcgt
8881 ccattcgcca ttcaggatcg aattaattct taattaacat catcaataat atacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 TTTTGGATTGAAGCCAATATGATAATGAGGGGGTGGAGTTTGTGACGTGGCGCGGGGCGT 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 GGGAACGGGGCGGGTGACGTAGTAGTGTGGCGGAAGTGTGATGTTGCAAGTGTGGCGGAA 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 CACATGTAAGCGACGGATGTGGCAAAAGTGACGTTTTTGGTGTGCGCCGGTGTACACAGG 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 AAGTGACAATTTTCGCGCGGTTTTAGGCGGATGTTGTAGTAAATTTGGGCGTAACCGAGT 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 AAGATTTGGCCATTTTCGCGGGAAAACTGAATAAGAGGAAGTGAAATCTGAATAATTTTG 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 TGTTACTCATAGCGCGTAATACGGCAGACCTCAGCGCTAGATTATTGAAGCATTTATCAG 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 GGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGG 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 GTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTAACTATAACGGTCCTAAGGTA 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 GCGAAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAG 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 CAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGG 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 TACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACT 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 GAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCT 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 CTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTT 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 AAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGAC 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 TCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGC 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 GCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTC 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 GGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAA 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 TTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGG 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 GGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATA 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 AATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCC 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 TGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGA 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 CAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATC 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 AAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACA 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 AAAGTAAGACCACCGCACAGCAAGCCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAG 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 GGACATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTA 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 GCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGA 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 GCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACG 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 CTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTG 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 AGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTC 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 CAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGG 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 GGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAAT 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 AAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAAC 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 AATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAAT 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 GAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACA 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 AATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGA 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 ATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCG 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 TTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAA 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 GGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGA 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 AAGCTTGGGATTCGAATTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAG 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 AATAGTAGACATAATAGCAACAGACATACAAACTAAAGAACTACAAAAACAAATTACAAA 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 AATTCAAAATTTTCGGGTTTTTCGAACCTAGGGTTCCGCGTTACATAACTTACGGTAAAT 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 GGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTT 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 CCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAA 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 ACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTC 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 AATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCT 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 ACTTGGCAGTACATCTACGTTTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTG 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 ACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACA 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_3 rf(3)(2901,3983)>>>
|
CMV prom(2885,2966)>>>
| |
2881 ACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 GAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCC 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 ATAGAAGAACCGAGTTTAAACTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAG 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
ORF_5 rf(5)(3072,4046)<<< ApaI
| |
3061 AGCTAGCGATATCAACAAGTTTGTACAAAAAAGTTGGCATGGCCACCACTGGGGCCCTGG 3120
TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACCGGTGGTGACCCCGGGACC
3121 GCAACTACTACGTGGACTCGTTCCTGCTGGGCGCCGACGCCGCGGATGAGCTGAGCGTTG 3180
CGTTGATGATGCACCTGAGCAAGGACGACCCGCGGCTGCGGCGCCTACTCGACTCGCAAC
3181 GCCGCTATGCGCCGGGGACCCTGGGCCAGCCTCCCCGGCAGGCGGCGACGCTGGCCGAGC 3240
CGGCGATACGCGGCCCCTGGGACCCGGTCGGAGGGGCCGTCCGCCGCTGCGACCGGCTCG
3241 ACCCCGACTTCAGCCCGTGCAGCTTCCAGTCCAAGGCGACGGTGTTTGGCGCCTCGTGGA 3300
TGGGGCTGAAGTCGGGCACGTCGAAGGTCAGGTTCCGCTGCCACAAACCGCGGAGCACCT
3301 ACCCAGTGCACGCGGCGGGCGCCAACGCTGTACCCGCTGCGGTGTACCACCACCATCACC 3360
TGGGTCACGTGCGCCGCCCGCGGTTGCGACATGGGCGACGCCACATGGTGGTGGTAGTGG
3361 ACCACCCCTACGTGCACCCCCAGGCGCCCGTGGCGGCGGCGGCGCCGGACGGCAGGTACA 3420
TGGTGGGGATGCACGTGGGGGTCCGCGGGCACCGCCGCCGCCGCGGCCTGCCGTCCATGT
3421 TGCGCTCCTGGCTGGAGCCCACGCCCGGTGCGCTCTCCTTCGCGGGCTTGCCCTCCAGCC 3480
ACGCGAGGACCGACCTCGGGTGCGGGCCACGCGAGAGGAAGCGCCCGAACGGGAGGTCGG
3481 GGCCTTATGGCATTAAACCTGAACCGCTGTCGGCCAGAAGGGGTGACTGTCCCACGCTTG 3540
CCGGAATACCGTAATTTGGACTTGGCGACAGCCGGTCTTCCCCACTGACAGGGTGCGAAC
3541 ACACTCACACTTTGTCCCTGACTGACTATGCTTGTGGTTCTCCTCCAGTTGATAGAGAAA 3600
TGTGAGTGTGAAACAGGGACTGACTGATACGAACACCAAGAGGAGGTCAACTATCTCTTT
3601 AACAACCCAGCGAAGGCGCCTTCTCTGAAAACAATGCTGAGAATGAGAGCGGCGGAGACA 3660
TTGTTGGGTCGCTTCCGCGGAAGAGACTTTTGTTACGACTCTTACTCTCGCCGCCTCTGT
ClaI
|
3661 AGCCCCCCATCGATCCCAATAACCCAGCAGCCAACTGGCTTCATGCGCGCTCCACTCGGA 3720
TCGGGGGGTAGCTAGGGTTATTGGGTCGTCGGTTGACCGAAGTACGCGCGAGGTGAGCCT
3721 AAAAGCGGTGCCCCTATACAAAACACCAGACCCTGGAACTGGAGAAAGAGTTTCTGTTCA 3780
TTTTCGCCACGGGGATATGTTTTGTGGTCTGGGACCTTGACCTCTTTCTCAAAGACAAGT
3781 ACATGTACCTCACCAGGGACCGCAGGTACGAGGTGGCTCGACTGCTCAACCTCACCGAGA 3840
TGTACATGGAGTGGTCCCTGGCGTCCATGCTCCACCGAGCTGACGAGTTGGAGTGGCTCT
3841 GGCAGGTCAAGATCTGGTTCCAGAACCGCAGGATGAAAATGAAGAAAATCAACAAAGACC 3900
CCGTCCAGTTCTAGACCAAGGTCTTGGCGTCCTACTTTTACTTCTTTTAGTTGTTTCTGG
XhoI
|
3901 GAGCAAAAGACGAGTGCCCAACTTTCTTGTACAAAGTGGTTGATATCCTCGAGTACCCAT 3960
CTCGTTTTCTGCTCACGGGTTGAAAGAACATGTTTCACCAACTATAGGAGCTCATGGGTA
SV40 prom(3998,4266)>>>
|
3961 ACGACGTCCCAGACTACGCTTGAGTTTAAACACGCGTGGTGTGGAAAGTCCCCAGGCTCC 4020
TGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGG
4021 CCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAG 4080
GGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTC
4081 TCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACC 4140
AGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGG
SV40 origin(4165,4242)>>>
|
4141 ATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCT 4200
TATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGA
SfiI
|
ORF_2 rf(2)(4232,4900)>>>
| |
4201 CCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCT 4260
GGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGA
puro marker(4301,4900)>>>
|
4261 GAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCATGACCGAGTACAAGCCCAC 4320
CTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTG
4321 GGTGCGCCTCGCCACCCGCGACGACGTCCCTCGGGCCGTACGCACCCTCGCCGCCGCGTT 4380
CCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAA
4381 CGCCGACTACCCCGCCACGCGCCACACCGTGGACCCGGACCGCCACATCGAGCGGGTCAC 4440
GCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTG
4441 CGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGC 4500
GCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCG
4501 GGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGT 4560
CCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCA
4561 GTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCA 4620
CAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGT
4621 ACAGATGGAAGGGCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCAC 4680
TGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTG
4681 CGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGG 4740
GCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCC
4741 AGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAA 4800
TCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTT
4801 CCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGG 4860
GGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCC
4861 ACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAACGCGTTCCGGAAATCAACC 4920
TGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGG
4921 TCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTAC 4980
AGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATG
4981 GCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTT 5040
CGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAA
5041 CATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGT 5100
GTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCA
5101 TGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGG 5160
ACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCC
5161 CATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCAC 5220
GTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTG
5221 GGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCAC 5280
CCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTG
5281 TGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGT 5340
ACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACA
5341 TGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGC 5400
ACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCG
5401 GGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTCGCCTTCGC 5460
CCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCG
delta_U3 other(5510,5562)>>>
|
5461 CCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCCGGATGGAAGGGCTA 5520
GGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGAT
HIV-1_5_LTR other(5563,5743)>>>
|
5521 ATTCACTCCCAACGAATACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGAC 5580
TAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTG
5581 CAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAA 5640
GTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATT
5641 AGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAG 5700
TCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATC
5701 AGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTAGTAGTTCATGTCAT 5760
TCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTA
5761 CTTATTATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTG 5820
GAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAAC
5821 TTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAA 5880
AAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTT
5881 GCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCAT 5940
CGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTA
5941 GTCTGGCATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCATTATGGGTATTATG 6000
CAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATAC
6001 GGTCTGCATTAATGAATCGGCCAACGATCCCGGTGTGAAATACCGCACAGATGCGTAAGG 6060
CCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCC
6061 AGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTC 6120
TCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAG
6121 GTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAA 6180
CAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTT
6181 TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGT 6240
AGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCA
pBR322 origin(6249,6868)<<<
|
6241 AAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA 6300
TTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTT
6301 AATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTT 6360
TTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAA
6361 CCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTG 6420
GGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGAC
6421 TCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTC 6480
AGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAG
6481 AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCC 6540
TCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGG
6541 GACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTA 6600
CTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAAT
6601 TCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCT 6660
AGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGA
6661 ACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATC 6720
TGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAG
6721 TGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAA 6780
ACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTT
6781 CAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAA 6840
GTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTT
6841 AAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAA 6900
TTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTT
6901 AACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTT 6960
TTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAA
6961 TTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGAC 7020
AATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTG
7021 AGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCC 7080
TCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGG
7081 ATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGC 7140
TATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCG
7141 CCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATA 7200
GGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTAT
7201 AACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATC 7260
TTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAG
7261 CAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGC 7320
GTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCG
7321 AACGTTGTTGAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGA 7380
TTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCT
7381 AACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAA 7440
TTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTT
7441 GCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGG 7500
CGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCC
NEOKAN prom(7521,7570)>>>
|
7501 TTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGA 7560
AAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCT
7561 AGCCCTGCAAAGTAAACTGGATGGCTTTCTCGCCGCCAAGGATCTGATGGCGCAGGGGAT 7620
TCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTA
NTP_II marker(7662,8450)>>>
|
BclI ORF_4 rf(3)(7659,8453)>>>
| | |
7621 CAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGC 7680
GTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACG
7681 ACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGA 7740
TGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCT
7741 CAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTT 7800
GTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAA
7801 TTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTAT 7860
AACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATA
7861 CGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGG 7920
GCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCC
7921 GAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTG 7980
CTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAAC
7981 CTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATC 8040
GAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAG
8041 CGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGA 8100
GCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCT
8101 TGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAG 8160
ACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTC
8161 CCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCC 8220
GGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGG
8221 ATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCG 8280
TACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGC
8281 ACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATA 8340
TGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTAT
8341 TTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCG 8400
AACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGC
8401 CTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTTTGT 8460
GAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACA
8461 TAAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAACATCCCT 8520
ATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGA
f1 origin(8558,8863)<<<
|
8521 TATAAATCAAAAGAATAGACCGCGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGT 8580
ATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCA
8581 CCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGAT 8640
GGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTA
8641 GGCCCACTACGTGAACCATCACCCAAATCAAGTTTTTTGCGGTCGAGGTGCCGTAAAGCT 8700
CCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGA
8701 CTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAAC 8760
GATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTG
8761 GTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTA 8820
CACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACAT
8821 GCGGTCACGCTGCGCGTAACCACCACACCCGCGCGCTTAATGCGCCGCTACAGGGCGCGT 8880
CGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCA
8881 CCATTCGCCATTCAGGATCGAATTAATTCTTAATTAACATCATCAATAATATACCTT 8937
GGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 8937 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3983
/sequence="ORF_3 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 3072..4046
/sequence="ORF_5 rf(5)"
misc_binding 3112..3117
/dbxref="REBASE:ApaI"
misc_binding 3669..3674
/dbxref="REBASE:ClaI"
misc_binding 3948..3953
/dbxref="REBASE:XhoI"
Promoter 3998..4266
/gene="SV40 prom"
Rep_Origin 4165..4242
/gene="SV40 origin"
ORF 4232..4900
/sequence="ORF_2 rf(2)"
misc_binding 4245..4257
/dbxref="REBASE:SfiI"
Marker 4301..4900
/gene="puro marker"
Other Gene 5510..5562
/gene="delta_U3 other"
Other Gene 5563..5743
/gene="HIV-1_5_LTR other"
Rep_Origin 6249..6868
/gene="pBR322 origin"
Promoter 7521..7570
/gene="NEOKAN prom"
misc_binding 7628..7633
/dbxref="REBASE:BclI"
ORF 7659..8453
/sequence="ORF_4 rf(3)"
Marker 7662..8450
/gene="NTP_II marker"
Rep_Origin 8558..8863
/gene="f1 origin"
BASE COUNT 2232 a 2264 c 2418 g 2023 t 0 others