pLenti-HOXA9-GFP
| Vector Name | pLenti-HOXA9-GFP |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat ggccaccact ggggccctgg
3121 gcaactacta cgtggactcg ttcctgctgg gcgccgacgc cgcggatgag ctgagcgttg
3181 gccgctatgc gccggggacc ctgggccagc ctccccggca ggcggcgacg ctggccgagc
3241 accccgactt cagcccgtgc agcttccagt ccaaggcgac ggtgtttggc gcctcgtgga
3301 acccagtgca cgcggcgggc gccaacgctg tacccgctgc ggtgtaccac caccatcacc
3361 accaccccta cgtgcacccc caggcgcccg tggcggcggc ggcgccggac ggcaggtaca
3421 tgcgctcctg gctggagccc acgcccggtg cgctctcctt cgcgggcttg ccctccagcc
3481 ggccttatgg cattaaacct gaaccgctgt cggccagaag gggtgactgt cccacgcttg
3541 acactcacac tttgtccctg actgactatg cttgtggttc tcctccagtt gatagagaaa
3601 aacaacccag cgaaggcgcc ttctctgaaa acaatgctga gaatgagagc ggcggagaca
3661 agccccccat cgatcccaat aacccagcag ccaactggct tcatgcgcgc tccactcgga
3721 aaaagcggtg cccctataca aaacaccaga ccctggaact ggagaaagag tttctgttca
3781 acatgtacct caccagggac cgcaggtacg aggtggctcg actgctcaac ctcaccgaga
3841 ggcaggtcaa gatctggttc cagaaccgca ggatgaaaat gaagaaaatc aacaaagacc
3901 gagcaaaaga cgagtgccca actttcttgt acaaagtggt tgatatctga ggatcctcta
3961 gaggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
4021 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
4081 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
4141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
4201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
4261 aggcctgtcg aggccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
4321 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
4381 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
4441 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
4501 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
4561 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
4621 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
4681 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
4741 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
4801 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
4861 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
4921 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
4981 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
5041 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
5101 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
5161 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
5221 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
5281 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
5341 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
5401 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
5461 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
5521 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
5581 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
5641 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga
5701 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
5761 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
5821 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
5881 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
5941 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
6001 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
6061 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
6121 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
6181 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
6241 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gtccggatgg
6301 aagggctaat tcactcccaa cgaatacaag atctgctttt tgcttgtact gggtctctct
6361 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
6421 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
6481 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
6541 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
6601 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
6661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
6721 cttatcatgt ctggcatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg
6781 gtattatggg tctgcattaa tgaatcggcc aacgatcccg gtgtgaaata ccgcacagat
6841 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
6901 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
6961 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
7021 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
7081 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
7141 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
7201 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
7261 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
7321 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
7381 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
7441 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
7501 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
7561 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
7621 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
7681 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
7741 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
7801 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
7861 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
7921 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
7981 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
8041 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
8101 gtttgcgcaa cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag
8161 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
8221 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
8281 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
8341 ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg
8401 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
8461 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
8521 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
8581 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
8641 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
8701 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
8761 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
8821 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
8881 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
8941 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
9001 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
9061 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
9121 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
9181 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
9241 aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
9301 acatccctta taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga
9361 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
9421 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc
9481 gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
9541 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
9601 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca
9661 gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat
9721 acctt
//
LOCUS dna 9725 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3950
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 3072..3704
/sequence="ORF_5 rf(4)"
misc_binding 3669..3674
/dbxref="REBASE:ClaI"
misc_binding 3951..3956
/dbxref="REBASE:BamHI"
misc_binding 3957..3962
/dbxref="REBASE:XbaI"
Promoter 3963..4231
/gene="SV40 prom"
Rep_Origin 4130..4207
/gene="SV40 origin"
misc_binding 4210..4222
/dbxref="REBASE:SfiI"
ORF 4279..5688
/sequence="ORF_2 rf(1)"
misc_binding 4852..4857
/dbxref="REBASE:PstI"
Marker 5089..5688
/gene="puro marker"
Other Gene 6298..6350
/gene="delta_U3 other"
Other Gene 6351..6531
/gene="HIV-1_5_LTR other"
Rep_Origin 7037..7656
/gene="pBR322 origin"
Promoter 8309..8358
/gene="NEOKAN prom"
misc_binding 8416..8421
/dbxref="REBASE:BclI"
ORF 8447..9241
/sequence="ORF_3 rf(2)"
Marker 8450..9238
/gene="NTP_II marker"
Rep_Origin 9346..9651
/gene="f1 origin"
BASE COUNT 2392 a 2541 c 2656 g 2136 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat ggccaccact ggggccctgg
3121 gcaactacta cgtggactcg ttcctgctgg gcgccgacgc cgcggatgag ctgagcgttg
3181 gccgctatgc gccggggacc ctgggccagc ctccccggca ggcggcgacg ctggccgagc
3241 accccgactt cagcccgtgc agcttccagt ccaaggcgac ggtgtttggc gcctcgtgga
3301 acccagtgca cgcggcgggc gccaacgctg tacccgctgc ggtgtaccac caccatcacc
3361 accaccccta cgtgcacccc caggcgcccg tggcggcggc ggcgccggac ggcaggtaca
3421 tgcgctcctg gctggagccc acgcccggtg cgctctcctt cgcgggcttg ccctccagcc
3481 ggccttatgg cattaaacct gaaccgctgt cggccagaag gggtgactgt cccacgcttg
3541 acactcacac tttgtccctg actgactatg cttgtggttc tcctccagtt gatagagaaa
3601 aacaacccag cgaaggcgcc ttctctgaaa acaatgctga gaatgagagc ggcggagaca
3661 agccccccat cgatcccaat aacccagcag ccaactggct tcatgcgcgc tccactcgga
3721 aaaagcggtg cccctataca aaacaccaga ccctggaact ggagaaagag tttctgttca
3781 acatgtacct caccagggac cgcaggtacg aggtggctcg actgctcaac ctcaccgaga
3841 ggcaggtcaa gatctggttc cagaaccgca ggatgaaaat gaagaaaatc aacaaagacc
3901 gagcaaaaga cgagtgccca actttcttgt acaaagtggt tgatatctga ggatcctcta
3961 gaggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
4021 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
4081 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
4141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
4201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
4261 aggcctgtcg aggccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
4321 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
4381 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
4441 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
4501 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
4561 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
4621 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
4681 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
4741 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
4801 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
4861 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
4921 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
4981 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
5041 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
5101 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
5161 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
5221 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
5281 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
5341 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
5401 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
5461 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
5521 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
5581 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
5641 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga
5701 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
5761 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
5821 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
5881 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
5941 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
6001 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
6061 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
6121 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
6181 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
6241 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gtccggatgg
6301 aagggctaat tcactcccaa cgaatacaag atctgctttt tgcttgtact gggtctctct
6361 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
6421 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
6481 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
6541 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
6601 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
6661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
6721 cttatcatgt ctggcatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg
6781 gtattatggg tctgcattaa tgaatcggcc aacgatcccg gtgtgaaata ccgcacagat
6841 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
6901 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
6961 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
7021 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
7081 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
7141 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
7201 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
7261 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
7321 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
7381 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
7441 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
7501 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
7561 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
7621 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
7681 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
7741 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
7801 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
7861 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
7921 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
7981 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
8041 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
8101 gtttgcgcaa cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag
8161 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
8221 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
8281 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
8341 ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg
8401 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
8461 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
8521 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
8581 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
8641 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
8701 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
8761 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
8821 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
8881 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
8941 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
9001 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
9061 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
9121 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
9181 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
9241 aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
9301 acatccctta taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga
9361 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
9421 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc
9481 gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
9541 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
9601 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca
9661 gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat
9721 acctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_4 rf(3)(2901,3950)>>>
|
CMV prom(2885,2966)>>>
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
ORF_5 rf(4)(3072,3704)<<<
|
3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGGCCACCACTGGGGCCCTGG 3120
TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACCGGTGGTGACCCCGGGACC
3121 GCAACTACTACGTGGACTCGTTCCTGCTGGGCGCCGACGCCGCGGATGAGCTGAGCGTTG 3180
CGTTGATGATGCACCTGAGCAAGGACGACCCGCGGCTGCGGCGCCTACTCGACTCGCAAC
3181 GCCGCTATGCGCCGGGGACCCTGGGCCAGCCTCCCCGGCAGGCGGCGACGCTGGCCGAGC 3240
CGGCGATACGCGGCCCCTGGGACCCGGTCGGAGGGGCCGTCCGCCGCTGCGACCGGCTCG
3241 ACCCCGACTTCAGCCCGTGCAGCTTCCAGTCCAAGGCGACGGTGTTTGGCGCCTCGTGGA 3300
TGGGGCTGAAGTCGGGCACGTCGAAGGTCAGGTTCCGCTGCCACAAACCGCGGAGCACCT
3301 ACCCAGTGCACGCGGCGGGCGCCAACGCTGTACCCGCTGCGGTGTACCACCACCATCACC 3360
TGGGTCACGTGCGCCGCCCGCGGTTGCGACATGGGCGACGCCACATGGTGGTGGTAGTGG
3361 ACCACCCCTACGTGCACCCCCAGGCGCCCGTGGCGGCGGCGGCGCCGGACGGCAGGTACA 3420
TGGTGGGGATGCACGTGGGGGTCCGCGGGCACCGCCGCCGCCGCGGCCTGCCGTCCATGT
3421 TGCGCTCCTGGCTGGAGCCCACGCCCGGTGCGCTCTCCTTCGCGGGCTTGCCCTCCAGCC 3480
ACGCGAGGACCGACCTCGGGTGCGGGCCACGCGAGAGGAAGCGCCCGAACGGGAGGTCGG
3481 GGCCTTATGGCATTAAACCTGAACCGCTGTCGGCCAGAAGGGGTGACTGTCCCACGCTTG 3540
CCGGAATACCGTAATTTGGACTTGGCGACAGCCGGTCTTCCCCACTGACAGGGTGCGAAC
3541 ACACTCACACTTTGTCCCTGACTGACTATGCTTGTGGTTCTCCTCCAGTTGATAGAGAAA 3600
TGTGAGTGTGAAACAGGGACTGACTGATACGAACACCAAGAGGAGGTCAACTATCTCTTT
3601 AACAACCCAGCGAAGGCGCCTTCTCTGAAAACAATGCTGAGAATGAGAGCGGCGGAGACA 3660
TTGTTGGGTCGCTTCCGCGGAAGAGACTTTTGTTACGACTCTTACTCTCGCCGCCTCTGT
ClaI
|
3661 AGCCCCCCATCGATCCCAATAACCCAGCAGCCAACTGGCTTCATGCGCGCTCCACTCGGA 3720
TCGGGGGGTAGCTAGGGTTATTGGGTCGTCGGTTGACCGAAGTACGCGCGAGGTGAGCCT
3721 AAAAGCGGTGCCCCTATACAAAACACCAGACCCTGGAACTGGAGAAAGAGTTTCTGTTCA 3780
TTTTCGCCACGGGGATATGTTTTGTGGTCTGGGACCTTGACCTCTTTCTCAAAGACAAGT
3781 ACATGTACCTCACCAGGGACCGCAGGTACGAGGTGGCTCGACTGCTCAACCTCACCGAGA 3840
TGTACATGGAGTGGTCCCTGGCGTCCATGCTCCACCGAGCTGACGAGTTGGAGTGGCTCT
3841 GGCAGGTCAAGATCTGGTTCCAGAACCGCAGGATGAAAATGAAGAAAATCAACAAAGACC 3900
CCGTCCAGTTCTAGACCAAGGTCTTGGCGTCCTACTTTTACTTCTTTTAGTTGTTTCTGG
BamHI XbaI
| |
3901 GAGCAAAAGACGAGtgcccaactttcttgtacaaagtggttgatatctgaggatcctcta 3960
CTCGTTTTCTGCTCACGGGTTGAAAGAACATGTTTCACCAACTATAGACTCCTAGGAGAT
SV40 prom(3963,4231)>>>
|
3961 gaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaa 4020
CTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTT
4021 ttagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaag 4080
AATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTC
SV40 origin(4130,4207)>>>
|
4081 catgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccct 4140
GTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGA
4141 aactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgc 4200
TTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACG
SfiI
|
4201 agaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttgg 4260
TCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACC
ORF_2 rf(1)(4279,5688)>>>
|
4261 aggcctgtcgaggccaccatggagagcgacgagagcggcctgcccgccatggagatcgag 4320
TCCGGACAGCTCCGGTGGTACCTCTCGCTGCTCTCGCCGGACGGGCGGTACCTCTAGCTC
4321 tgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggc 4380
ACGGCGTAGTGGCCGTGGGACTTGCCGCACCTCAAGCTCGACCACCCGCCGCCTCTCCCG
4381 acccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttc 4440
TGGGGGTTCGTCCCGGCGTACTGGTTGTTCTACTTCTCGTGGTTTCCGCGGGACTGGAAG
4441 agcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctacccc 4500
TCGGGGATGGACGACTCGGTGCACTACCCGATGCCGAAGATGGTGAAGCCGTGGATGGGG
4501 agcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgc 4560
TCGCCGATGCTCTTGGGGAAGGACGTGCGGTAGTTGTTGCCGCCGATGTGGTTGTGGGCG
4561 atcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggcc 4620
TAGCTCTTCATGCTCCTGCCGCCGCACGACGTGCACTCGAAGTCGATGGCGATGCTCCGG
4621 ggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatc 4680
CCGGCGCACTAGCCGCTGAAGTTCCACCACCCGTGGCCGAAGGGGCTCCTGTCGCACTAG
4681 ttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgat 4740
AAGTGGCTGTTCTAGTAGGCGTCGTTGCGGTGGCACCTCGTGGACGTGGGGTACCCGCTA
4741 aacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagc 4800
TTGCACGACCACCCGTCGAAGCGGGCGTGGAAGTCGGACGCGCTGCCGCCGATGATGTCG
PstI
|
4801 ttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaac 4860
AAGCACCACCTGTCGGTGTACGTGAAGTTCTCGCGGTAGGTGGGGTCGTAGGACGTCTTG
4861 gggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggc 4920
CCCCCGGGGTACAAGCGGAAGGCGGCGCACCTCCTCGACGTGTCGTTGTGGCTCGACCCG
4921 atcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcag 4980
TAGCACCTCATGGTCGTGCGGAAGTTCTGGGGGTAGCGGAAGCGGTCTAGGGCGCGAGTC
4981 tcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgcgagggc 5040
AGCAGGTTAAGACGGCACCTGCCGTGGCGGCCTGGGCCGAGGTGGCCTAGAGCGCTCCCG
puro marker(5089,5688)>>>
|
5041 agaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctatgaccgagtac 5100
TCTCCTTCAGAAGATTGTACGCCACTGCACCTCCTCTTAGGGCCGGGATACTGGCTCATG
5101 aagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgcc 5160
TTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGG
5161 gccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgag 5220
CGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTC
5221 cgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtg 5280
GCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCAC
5281 tgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcg 5340
ACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGC
5341 ggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggcc 5400
CCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGG
5401 gcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttc 5460
CGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAG
5461 ctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtg 5520
GACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCAC
5521 ctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcg 5580
GAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGC
5581 ccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtg 5640
GGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCAC
5641 cccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccgga 5700
GGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCT
5701 aatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgct 5760
TTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGA
5761 ccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgt 5820
GGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCA
5821 atggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttg 5880
TACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAAC
5881 tggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccact 5940
ACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGA
5941 ggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccct 6000
CCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGA
6001 attgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctg 6060
TAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGAC
6061 ttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctc 6120
AACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAG
6121 gcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctc 6180
CGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAG
6181 aatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctc 6240
TTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAG
delta_U3 other(6298,6350)>>>
|
6241 gccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatgg 6300
CGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACC
HIV-1_5_LTR other(6351,6531)>>>
|
6301 aagggctaattcactcccaacgaatacaagatctgctttttgcttgtactgggtctctct 6360
TTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGA
6361 ggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagc 6420
CCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCG
6421 ctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctg 6480
GAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGAC
6481 gtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagtt 6540
CATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAA
6541 catgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgaga 6600
GTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCT
6601 ggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttca 6660
CCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGT
6661 caaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtat 6720
GTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATA
6721 cttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgg 6780
GAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACC
6781 gtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagat 6840
CATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTA
6841 gcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgc 6900
CGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACG
6901 gctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttat 6960
CGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATA
6961 ccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcca 7020
GGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGT
pBR322 origin(7037,7656)<<<
|
7021 ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagc 7080
CCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCG
7081 atcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacc 7140
TAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGG
7141 aggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccg 7200
TCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGC
7201 gatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgta 7260
CTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACAT
7261 ggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccg 7320
CCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGC
7321 ttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagac 7380
AAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTG
7381 acgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtag 7440
TGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATC
7441 gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtat 7500
CGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATA
7501 ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgat 7560
AACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTA
7561 ccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgc 7620
GGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCG
7621 gcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagt 7680
CGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCA
7681 ggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacct 7740
CCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGA
7741 agatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaactt 7800
TCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAA
7801 ggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttc 7860
CCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAG
7861 gttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttac 7920
CAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATG
7921 catctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat 7980
GTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATA
7981 cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccg 8040
GTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGC
8041 cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaata 8100
GGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTAT
8101 gtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccag 8160
CAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTC
8161 tccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaaggga 8220
AGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCT
8221 aaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctaga 8280
TTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCT
NEOKAN prom(8309,8358)>>>
|
8281 ctgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaa 8340
GACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATT
8341 ggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcg 8400
CCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGC
NTP_II marker(8450,9238)>>>
|
BclI ORF_3 rf(2)(8447,9241)>>>
| | |
8401 caggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaaga 8460
GTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCT
8461 tggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggc 8520
ACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCG
8521 acaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgccc 8580
TGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGG
8581 ggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagc 8640
CCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCG
8641 gcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcac 8700
CGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTG
8701 tgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatc 8760
ACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAG
8761 tcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatac 8820
AGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATG
8821 gcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacg 8880
CGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGC
8881 tactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggct 8940
ATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGA
8941 cgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgt 9000
GCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCA
9001 cgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctgg 9060
GCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACC
9061 attcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctac 9120
TAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATG
9121 ccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacgg 9180
GGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCC
9181 tatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctg 9240
ATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGAC
9241 aattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggca 9300
TTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGT
f1 origin(9346,9651)<<<
|
9301 acatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttgga 9360
TGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCT
9361 acaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatc 9420
TGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAG
9421 agggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgcc 9480
TCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGG
9481 gtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagc 9540
CATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCG
9541 cggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctgg 9600
GCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACC
9601 caagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctaca 9660
GTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGT
9661 gggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatat 9720
CCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATA
9721 acctt 9725
TGGAA
LOCUS dna 9725 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3950
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 3072..3704
/sequence="ORF_5 rf(4)"
misc_binding 3669..3674
/dbxref="REBASE:ClaI"
misc_binding 3951..3956
/dbxref="REBASE:BamHI"
misc_binding 3957..3962
/dbxref="REBASE:XbaI"
Promoter 3963..4231
/gene="SV40 prom"
Rep_Origin 4130..4207
/gene="SV40 origin"
misc_binding 4210..4222
/dbxref="REBASE:SfiI"
ORF 4279..5688
/sequence="ORF_2 rf(1)"
misc_binding 4852..4857
/dbxref="REBASE:PstI"
Marker 5089..5688
/gene="puro marker"
Other Gene 6298..6350
/gene="delta_U3 other"
Other Gene 6351..6531
/gene="HIV-1_5_LTR other"
Rep_Origin 7037..7656
/gene="pBR322 origin"
Promoter 8309..8358
/gene="NEOKAN prom"
misc_binding 8416..8421
/dbxref="REBASE:BclI"
ORF 8447..9241
/sequence="ORF_3 rf(2)"
Marker 8450..9238
/gene="NTP_II marker"
Rep_Origin 9346..9651
/gene="f1 origin"
BASE COUNT 2392 a 2541 c 2656 g 2136 t 0 others