pLenti-HOXB8-GFP
| Vector Name | pLenti-HOXB8-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gagctcttat ttcgtcaact
3121 cactgttctc caaatacaaa accggggagt ccctgcgccc caattattat gactgcggct
3181 tcgcccagga cctgggcggc cgacccaccg tggtgtacgg tcccagcagc ggcggcagct
3241 tccagcaccc gtcgcaaatc caggagttct accacgggcc gtcgtcgctg tccacggctc
3301 cctaccagca gaacccgtgc gccgtggcgt gccacgggga ccccggcaat ttctacggct
3361 acgacccgct gcaacgccag agcctattcg gtgcgcagga tccagacctg gtgcagtacg
3421 cagactgcaa gcttgccgcc gccagcggcc tgggcgagga ggccgagggc tccgagcaga
3481 gcccgtcgcc cacacagctc ttcccctgga tgcgcccgca agcagccgcc ggacgcaggc
3541 gaggccgaca gacctacagc cgctaccaga ccctggagct ggagaaggag ttcctattta
3601 atccctatct gactcgtaag cggcgaatcg aggtatcgca cgccctggga ctgacagaga
3661 gacaggtcaa aatctggttc cagaaccgga ggatgaagtg gaaaaaagag aacaacaaag
3721 acaagttccc cagcagcaaa tgcgagcagg aggagctgga gaaacagaag ctggagcggg
3781 ccccagaggc ggcggacgag ggcgacgcgc agaagggcga caagaagtgc ccaactttct
3841 tgtacaaagt ggttgatatc tgaggatcct ctagaggtgt ggaaagtccc caggctcccc
3901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
3961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
4021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
4081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
4141 gctattccag aagtagtgag gaggcttttt tggaggcctg tcgaggccac catggagagc
4201 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
4261 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
4321 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
4381 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
4441 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
4501 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
4561 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
4621 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
4681 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
4741 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
4801 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
4861 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
4921 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
4981 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
5041 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
5101 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
5161 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
5221 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
5281 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
5341 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
5401 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
5461 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
5521 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
5581 acccgcaagc ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt
5641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
5701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
5761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
5821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
5881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
5941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
6001 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
6061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
6121 ctgctgccgg ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct
6181 ccctttgggc cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac
6241 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
6301 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
6361 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
6421 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
6481 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
6541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
6601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg
6661 tgcggagaaa gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg
6721 gccaacgatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
7381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat
8041 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
8101 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
8161 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
8221 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
8281 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac
8341 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
8401 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
8461 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
8521 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
8581 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
8641 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
8701 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
8761 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
8821 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
8881 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
8941 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
9001 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
9061 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
9121 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca
9181 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga
9241 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
9301 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
9361 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag
9421 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
9481 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
9541 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc
9601 gaattaattc ttaattaaca tcatcaataa tatacctt
LOCUS dna 9638 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3863
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3870..3875
/dbxref="REBASE:XbaI"
Promoter 3876..4144
/gene="SV40 prom"
Rep_Origin 4043..4120
/gene="SV40 origin"
misc_binding 4123..4135
/dbxref="REBASE:SfiI"
ORF 4192..5601
/sequence="ORF_2 rf(1)"
misc_binding 4765..4770
/dbxref="REBASE:PstI"
Marker 5002..5601
/gene="puro marker"
Other Gene 6211..6263
/gene="delta_U3 other"
Other Gene 6264..6444
/gene="HIV-1_5_LTR other"
Rep_Origin 6950..7569
/gene="pBR322 origin"
Promoter 8222..8271
/gene="NEOKAN prom"
misc_binding 8329..8334
/dbxref="REBASE:BclI"
ORF 8360..9154
/sequence="ORF_3 rf(2)"
Marker 8363..9151
/gene="NTP_II marker"
Rep_Origin 9259..9564
/gene="f1 origin"
BASE COUNT 2385 a 2498 c 2647 g 2108 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gagctcttat ttcgtcaact
3121 cactgttctc caaatacaaa accggggagt ccctgcgccc caattattat gactgcggct
3181 tcgcccagga cctgggcggc cgacccaccg tggtgtacgg tcccagcagc ggcggcagct
3241 tccagcaccc gtcgcaaatc caggagttct accacgggcc gtcgtcgctg tccacggctc
3301 cctaccagca gaacccgtgc gccgtggcgt gccacgggga ccccggcaat ttctacggct
3361 acgacccgct gcaacgccag agcctattcg gtgcgcagga tccagacctg gtgcagtacg
3421 cagactgcaa gcttgccgcc gccagcggcc tgggcgagga ggccgagggc tccgagcaga
3481 gcccgtcgcc cacacagctc ttcccctgga tgcgcccgca agcagccgcc ggacgcaggc
3541 gaggccgaca gacctacagc cgctaccaga ccctggagct ggagaaggag ttcctattta
3601 atccctatct gactcgtaag cggcgaatcg aggtatcgca cgccctggga ctgacagaga
3661 gacaggtcaa aatctggttc cagaaccgga ggatgaagtg gaaaaaagag aacaacaaag
3721 acaagttccc cagcagcaaa tgcgagcagg aggagctgga gaaacagaag ctggagcggg
3781 ccccagaggc ggcggacgag ggcgacgcgc agaagggcga caagaagtgc ccaactttct
3841 tgtacaaagt ggttgatatc tgaggatcct ctagaggtgt ggaaagtccc caggctcccc
3901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
3961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
4021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
4081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
4141 gctattccag aagtagtgag gaggcttttt tggaggcctg tcgaggccac catggagagc
4201 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
4261 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
4321 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
4381 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
4441 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
4501 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
4561 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
4621 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
4681 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
4741 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
4801 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
4861 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
4921 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
4981 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
5041 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
5101 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
5161 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
5221 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
5281 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
5341 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
5401 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
5461 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
5521 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
5581 acccgcaagc ccggtgcctg aacgcgttcc ggaaatcaac ctctggatta caaaatttgt
5641 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
5701 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
5761 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
5821 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
5881 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
5941 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
6001 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
6061 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
6121 ctgctgccgg ctctgcggcc tcttccgcgt ctcgccttcg ccctcagacg agtcggatct
6181 ccctttgggc cgcctccccg cctgtccgga tggaagggct aattcactcc caacgaatac
6241 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
6301 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
6361 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
6421 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
6481 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
6541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
6601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggcat ctatgtcggg
6661 tgcggagaaa gaggtaatga aatggcatta tgggtattat gggtctgcat taatgaatcg
6721 gccaacgatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
7381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat
8041 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
8101 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
8161 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
8221 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
8281 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac
8341 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
8401 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
8461 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
8521 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
8581 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
8641 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
8701 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
8761 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
8821 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
8881 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
8941 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
9001 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
9061 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
9121 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca
9181 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga
9241 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
9301 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
9361 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag
9421 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
9481 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
9541 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc
9601 gaattaattc ttaattaaca tcatcaataa tatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
ORF_4 rf(3)(2901,3863)>>>
|
CMV prom(2885,2966)>>>
| |
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
3061 agctagcgatatcaacaagtttgtacaaaaaagttggcATGAGCTCTTATTTCGTCAACT 3120
TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACTCGAGAATAAAGCAGTTGA
3121 CACTGTTCTCCAAATACAAAACCGGGGAGTCCCTGCGCCCCAATTATTATGACTGCGGCT 3180
GTGACAAGAGGTTTATGTTTTGGCCCCTCAGGGACGCGGGGTTAATAATACTGACGCCGA
3181 TCGCCCAGGACCTGGGCGGCCGACCCACCGTGGTGTACGGTCCCAGCAGCGGCGGCAGCT 3240
AGCGGGTCCTGGACCCGCCGGCTGGGTGGCACCACATGCCAGGGTCGTCGCCGCCGTCGA
3241 TCCAGCACCCGTCGCAAATCCAGGAGTTCTACCACGGGCCGTCGTCGCTGTCCACGGCTC 3300
AGGTCGTGGGCAGCGTTTAGGTCCTCAAGATGGTGCCCGGCAGCAGCGACAGGTGCCGAG
3301 CCTACCAGCAGAACCCGTGCGCCGTGGCGTGCCACGGGGACCCCGGCAATTTCTACGGCT 3360
GGATGGTCGTCTTGGGCACGCGGCACCGCACGGTGCCCCTGGGGCCGTTAAAGATGCCGA
3361 ACGACCCGCTGCAACGCCAGAGCCTATTCGGTGCGCAGGATCCAGACCTGGTGCAGTACG 3420
TGCTGGGCGACGTTGCGGTCTCGGATAAGCCACGCGTCCTAGGTCTGGACCACGTCATGC
3421 CAGACTGCAAGCTTGCCGCCGCCAGCGGCCTGGGCGAGGAGGCCGAGGGCTCCGAGCAGA 3480
GTCTGACGTTCGAACGGCGGCGGTCGCCGGACCCGCTCCTCCGGCTCCCGAGGCTCGTCT
3481 GCCCGTCGCCCACACAGCTCTTCCCCTGGATGCGCCCGCAAGCAGCCGCCGGACGCAGGC 3540
CGGGCAGCGGGTGTGTCGAGAAGGGGACCTACGCGGGCGTTCGTCGGCGGCCTGCGTCCG
3541 GAGGCCGACAGACCTACAGCCGCTACCAGACCCTGGAGCTGGAGAAGGAGTTCCTATTTA 3600
CTCCGGCTGTCTGGATGTCGGCGATGGTCTGGGACCTCGACCTCTTCCTCAAGGATAAAT
3601 ATCCCTATCTGACTCGTAAGCGGCGAATCGAGGTATCGCACGCCCTGGGACTGACAGAGA 3660
TAGGGATAGACTGAGCATTCGCCGCTTAGCTCCATAGCGTGCGGGACCCTGACTGTCTCT
3661 GACAGGTCAAAATCTGGTTCCAGAACCGGAGGATGAAGTGGAAAAAAGAGAACAACAAAG 3720
CTGTCCAGTTTTAGACCAAGGTCTTGGCCTCCTACTTCACCTTTTTTCTCTTGTTGTTTC
3721 ACAAGTTCCCCAGCAGCAAATGCGAGCAGGAGGAGCTGGAGAAACAGAAGCTGGAGCGGG 3780
TGTTCAAGGGGTCGTCGTTTACGCTCGTCCTCCTCGACCTCTTTGTCTTCGACCTCGCCC
3781 CCCCAGAGGCGGCGGACGAGGGCGACGCGCAGAAGGGCGACAAGAAGtgcccaactttct 3840
GGGGTCTCCGCCGCCTGCTCCCGCTGCGCGTCTTCCCGCTGTTCTTCACGGGTTGAAAGA
XbaI SV40 prom(3876,4144)>>>
| |
3841 tgtacaaagtggttgatatctgaggatcctctagaggtgtggaaagtccccaggctcccc 3900
ACATGTTTCACCAACTATAGACTCCTAGGAGATCTCCACACCTTTCAGGGGTCCGAGGGG
3901 agcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtc 3960
TCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAG
3961 cccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccat 4020
GGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTA
SV40 origin(4043,4120)>>>
|
4021 agtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctcc 4080
TCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGG
SfiI
|
4081 gccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctga 4140
CGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACT
ORF_2 rf(1)(4192,5601)>>>
|
4141 gctattccagaagtagtgaggaggcttttttggaggcctgtcgaggccaccatggagagc 4200
CGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGACAGCTCCGGTGGTACCTCTCG
4201 gacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggc 4260
CTGCTCTCGCCGGACGGGCGGTACCTCTAGCTCACGGCGTAGTGGCCGTGGGACTTGCCG
4261 gtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaac 4320
CACCTCAAGCTCGACCACCCGCCGCCTCTCCCGTGGGGGTTCGTCCCGGCGTACTGGTTG
4321 aagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatg 4380
TTCTACTTCTCGTGGTTTCCGCGGGACTGGAAGTCGGGGATGGACGACTCGGTGCACTAC
4381 ggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcac 4440
CCGATGCCGAAGATGGTGAAGCCGTGGATGGGGTCGCCGATGCTCTTGGGGAAGGACGTG
4441 gccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtg 4500
CGGTAGTTGTTGCCGCCGATGTGGTTGTGGGCGTAGCTCTTCATGCTCCTGCCGCCGCAC
4501 ctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtg 4560
GACGTGCACTCGAAGTCGATGGCGATGCTCCGGCCGGCGCACTAGCCGCTGAAGTTCCAC
4561 gtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaac 4620
CACCCGTGGCCGAAGGGGCTCCTGTCGCACTAGAAGTGGCTGTTCTAGTAGGCGTCGTTG
4621 gccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgc 4680
CGGTGGCACCTCGTGGACGTGGGGTACCCGCTATTGCACGACCACCCGTCGAAGCGGGCG
4681 accttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttc 4740
TGGAAGTCGGACGCGCTGCCGCCGATGATGTCGAAGCACCACCTGTCGGTGTACGTGAAG
PstI
|
4741 aagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgc 4800
TTCTCGCGGTAGGTGGGGTCGTAGGACGTCTTGCCCCCGGGGTACAAGCGGAAGGCGGCG
4801 gtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaag 4860
CACCTCCTCGACGTGTCGTTGTGGCTCGACCCGTAGCACCTCATGGTCGTGCGGAAGTTC
4861 acccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcacc 4920
TGGGGGTAGCGGAAGCGGTCTAGGGCGCGAGTCAGCAGGTTAAGACGGCACCTGCCGTGG
4921 gccggacccggctccaccggatctcgcgagggcagaggaagtcttctaacatgcggtgac 4980
CGGCCTGGGCCGAGGTGGCCTAGAGCGCTCCCGTCTCCTTCAGAAGATTGTACGCCACTG
puro marker(5002,5601)>>>
|
4981 gtggaggagaatcccggccctatgaccgagtacaagcccacggtgcgcctcgccacccgc 5040
CACCTCCTCTTAGGGCCGGGATACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCG
5041 gacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacg 5100
CTGCTGCAGGGGTCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGC
5101 cgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttc 5160
GCGGTGTGGCAGCTAGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAG
5161 ctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtg 5220
GAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCAC
5221 gcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccg 5280
CGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGC
5281 cgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctg 5340
GCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGAC
5341 gcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgac 5400
CGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTG
5401 caccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgc 5460
GTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCG
5461 gccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcgg 5520
CGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCC
5521 ctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatg 5580
GAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTAC
5581 acccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgt 5640
TGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACA
5641 gaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgct 5700
CTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGA
5701 ttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtat 5760
AATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATA
5761 aaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtg 5820
TTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCAC
5821 gtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcag 5880
CACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTC
5881 ctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcc 5940
GAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGG
5941 tgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttg 6000
ACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAAC
6001 tcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgc 6060
AGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCG
6061 gggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggc 6120
CCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCG
6121 ctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatct 6180
GACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGA
delta_U3 other(6211,6263)>>>
|
6181 ccctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatac 6240
GGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATG
HIV-1_5_LTR other(6264,6444)>>>
|
6241 aagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagc 6300
TTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCG
6301 tctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttc 6360
AGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAG
6361 aagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagaccctttt 6420
TTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAA
6421 agtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttat 6480
TCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATA
6481 aacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatg 6540
TTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTAC
6541 gttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcatt 6600
CAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAA
6601 ctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcggg 6660
GATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCC
6661 tgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcg 6720
ACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGC
6721 gccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggc 6780
CGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCG
6781 gctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg 6840
CGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCC
6841 tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa 6900
ATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTT
pBR322 origin(6950,7569)<<<
|
6901 agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgg 6960
TCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACC
6961 cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga 7020
GCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCT
7021 ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcg 7080
CCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGC
7081 tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg 7140
ACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCC
7141 gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttc 7200
CTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAG
7201 gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg 7260
CGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGC
7261 gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca 7320
CATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGT
7321 ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggt 7380
GACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCA
7381 ggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccag 7440
CCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTC
7441 ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg 7500
AATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGC
7501 gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc 7560
CACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAG
7561 ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt 7620
GAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAA
7621 tggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtt 7680
ACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAA
7681 ttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca 7740
AATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGT
7741 gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg 7800
CACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGC
7801 tcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatac 7860
AGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATG
7861 cgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaaggg 7920
GCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCC
7921 ccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc 7980
GGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGG
7981 gggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggat 8040
CCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTA
8041 cttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggat 8100
GAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTA
8101 gaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggt 8160
CTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCA
8161 agcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcga 8220
TCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCT
NEOKAN prom(8222,8271)>>>
|
8221 accggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactg 8280
TGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGAC
BclI
|
8281 gatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagac 8340
CTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTG
NTP_II marker(8363,9151)>>>
|
ORF_3 rf(2)(8360,9154)>>>
| |
8341 aggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgc 8400
TCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCG
8401 ttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgc 8460
AACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACG
8461 cgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtc 8520
GCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAG
8521 cggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacggg 8580
GCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCC
8581 cgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctatt 8640
GCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAA
8641 gggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatc 8700
CCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAG
8701 catcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcga 8760
GTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCT
8761 ccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcga 8820
GGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCT
8821 tcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggct 8880
AGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGA
8881 caaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgcc 8940
GTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGG
8941 gaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgt 9000
CTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACA
9001 ggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcgg 9060
CCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCC
9061 cgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcat 9120
GCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTA
9121 cgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatca 9180
GCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGT
9181 gctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaataga 9240
CGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCT
f1 origin(9259,9564)<<<
|
9241 ccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgg 9300
GGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACC
9301 actccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccat 9360
TGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTA
9361 cacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaag 9420
GTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTC
9421 ggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaaggga 9480
CCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCT
9481 agaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaa 9540
TCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATT
9541 ccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatc 9600
GGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAG
9601 gaattaattcttaattaacatcatcaataatatacctt 9638
CTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 9638 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3863
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3870..3875
/dbxref="REBASE:XbaI"
Promoter 3876..4144
/gene="SV40 prom"
Rep_Origin 4043..4120
/gene="SV40 origin"
misc_binding 4123..4135
/dbxref="REBASE:SfiI"
ORF 4192..5601
/sequence="ORF_2 rf(1)"
misc_binding 4765..4770
/dbxref="REBASE:PstI"
Marker 5002..5601
/gene="puro marker"
Other Gene 6211..6263
/gene="delta_U3 other"
Other Gene 6264..6444
/gene="HIV-1_5_LTR other"
Rep_Origin 6950..7569
/gene="pBR322 origin"
Promoter 8222..8271
/gene="NEOKAN prom"
misc_binding 8329..8334
/dbxref="REBASE:BclI"
ORF 8360..9154
/sequence="ORF_3 rf(2)"
Marker 8363..9151
/gene="NTP_II marker"
Rep_Origin 9259..9564
/gene="f1 origin"
BASE COUNT 2385 a 2498 c 2647 g 2108 t 0 others