pLenti-III-HPV-16-E6-E7-No Selection
| Vector Name | pLenti-III-HPV-16-E6-E7-No Selection |
| VectorType | Lentivector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV Sequencing Primer5`---CGC AAA TGG GCG GTA GGC GTG---3`Reverse Sequencing Primer5`---GAA AGC CAT ACG GGA AGC AAT AG---3` |
| Additional Information | DEFINITION Human papillomavirus type 16 isolate HPV16-32 E6 protein (E6) gene, complete cds. ACCESSION EU880266 DEFINITION Human papillomavirus type 16 isolate LL31 E7 protein gene, complete cds. ACCESSION JX412167Full E6/E7 Insert sequence: Atgcaccaaaagagaactgcaatgtttcaggacccacaggagcgacccagaaagttaccacagttatgcacagagc tgcaaacaactatacatgatataatattagaatgtgtgtactgcaagcaacagttactgcgacgtgaggtatatgacttt gcttttcgggatttatgcatagtatatagagatgggaatccatatgctgtatgtgataaatgtttaaagttttattctaa aattagtgagtatagacattattgttatagtttgtatggaacaacattagaacagcaatacaacaaaccgttgtgtgatt tgttaattaggtgtattaactgtcaaaagccactgtgtcctgaagaaaagcaaagacatctggacaaaaagcaaagattc cataatataaggggtcggtggaccggtcgatgtatgtcttgttgcagatcatcaagaacacgtagagaaacccagctgta atcatgcatggagatacacctacattgcatgaatatatgttagatttgcaaccagagacaactgatctctactgttatga gcaattaaatgacagctcagaggaggaggatgaaatagatggtccagctggacaagcagaaccggacagagcccattaca atattgtaaccttttgttgcaagtgtgactctacgcttcggttgtgcgtacaaagcacacacgtagacattcgtactttg gaagacctgttaatgggcacactaggaattgtgtgccccatctgttctcagaaaccataa |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc actagaggat ccttttatgc accaaaagag aactgcaatg tttcaggacc
3181 cacaggagcg acccagaaag ttaccacagt tatgcacaga gctgcaaaca actatacatg
3241 atataatatt agaatgtgtg tactgcaagc aacagttact gcgacgtgag gtatatgact
3301 ttgcttttcg ggatttatgc atagtatata gagatgggaa tccatatgct gtatgtgata
3361 aatgtttaaa gttttattct aaaattagtg agtatagaca ttattgttat agtttgtatg
3421 gaacaacatt agaacagcaa tacaacaaac cgttgtgtga tttgttaatt aggtgtatta
3481 actgtcaaaa gccactgtgt cctgaagaaa agcaaagaca tctggacaaa aagcaaagat
3541 tccataatat aaggggtcgg tggaccggtc gatgtatgtc ttgttgcaga tcatcaagaa
3601 cacgtagaga aacccagctg taatcatgca tggagataca cctacattgc atgaatatat
3661 gttagatttg caaccagaga caactgatct ctactgttat gagcaattaa atgacagctc
3721 agaggaggag gatgaaatag atggtccagc tggacaagca gaaccggaca gagcccatta
3781 caatattgta accttttgtt gcaagtgtga ctctacgctt cggttgtgcg tacaaagcac
3841 acacgtagac attcgtactt tggaagacct gttaatgggc acactaggaa ttgtgtgccc
3901 catctgttct cagaaaccat aatctaccat ggctgatcct gcaggtaccg gatccggtac
3961 cgagctcgaa ttcaagctta agtttatcgg gcccgcggcc gctggatcct ctagactgca
4021 gctcgagaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
4081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
4141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
4201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
4261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
4321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
4381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
4441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
4501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
4561 gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtc
4621 cggatggaag ggctaattca ctcccaacga atacaagatc tgctttttgc ttgtactggg
4681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
4741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
4801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
4861 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
4921 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
4981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
5041 aatgtatctt atcatgtctg gcatctatgt cgggtgcgga gaaagaggta atgaaatggc
5101 attatgggta ttatgggtct gcattaatga atcggccaac gatcccggtg tgaaataccg
5161 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
5221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
5281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
5341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
5401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
5461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
5521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
5581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
5641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
5701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
5761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
5821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
5881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
5941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
6001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
6061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
6121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
6181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
6241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
6301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
6361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
6421 gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga
6481 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
6541 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
6601 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
6661 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct
6721 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg
6781 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
6841 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
6901 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
6961 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
7021 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
7081 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
7141 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
7201 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
7261 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
7321 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
7381 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
7441 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
7501 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
7561 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
7621 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca
7681 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
7741 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg
7801 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
7861 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
7921 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc
7981 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca
8041 ataatatacc tt
LOCUS dna 8052 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
ORF 3929..4636
/sequence="ORF_2 rf(2)"
misc_binding 3989..3994
/dbxref="REBASE:ApaI"
misc_binding 3995..4002
/dbxref="REBASE:NotI"
misc_binding 4010..4015
/dbxref="REBASE:XbaI"
misc_binding 4022..4027
/dbxref="REBASE:XhoI"
Other Gene 4625..4677
/gene="delta_U3 other"
Other Gene 4678..4858
/gene="HIV-1_5_LTR other"
Rep_Origin 5364..5983
/gene="pBR322 origin"
Promoter 6636..6685
/gene="NEOKAN prom"
misc_binding 6743..6748
/dbxref="REBASE:BclI"
ORF 6774..7568
/sequence="ORF_3 rf(3)"
Marker 6777..7565
/gene="NTP_II marker"
Rep_Origin 7673..7978
/gene="f1 origin"
BASE COUNT 2158 a 1817 c 2104 g 1973 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc actagaggat ccttttatgc accaaaagag aactgcaatg tttcaggacc
3181 cacaggagcg acccagaaag ttaccacagt tatgcacaga gctgcaaaca actatacatg
3241 atataatatt agaatgtgtg tactgcaagc aacagttact gcgacgtgag gtatatgact
3301 ttgcttttcg ggatttatgc atagtatata gagatgggaa tccatatgct gtatgtgata
3361 aatgtttaaa gttttattct aaaattagtg agtatagaca ttattgttat agtttgtatg
3421 gaacaacatt agaacagcaa tacaacaaac cgttgtgtga tttgttaatt aggtgtatta
3481 actgtcaaaa gccactgtgt cctgaagaaa agcaaagaca tctggacaaa aagcaaagat
3541 tccataatat aaggggtcgg tggaccggtc gatgtatgtc ttgttgcaga tcatcaagaa
3601 cacgtagaga aacccagctg taatcatgca tggagataca cctacattgc atgaatatat
3661 gttagatttg caaccagaga caactgatct ctactgttat gagcaattaa atgacagctc
3721 agaggaggag gatgaaatag atggtccagc tggacaagca gaaccggaca gagcccatta
3781 caatattgta accttttgtt gcaagtgtga ctctacgctt cggttgtgcg tacaaagcac
3841 acacgtagac attcgtactt tggaagacct gttaatgggc acactaggaa ttgtgtgccc
3901 catctgttct cagaaaccat aatctaccat ggctgatcct gcaggtaccg gatccggtac
3961 cgagctcgaa ttcaagctta agtttatcgg gcccgcggcc gctggatcct ctagactgca
4021 gctcgagaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
4081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
4141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
4201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
4261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
4321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
4381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
4441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
4501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
4561 gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtc
4621 cggatggaag ggctaattca ctcccaacga atacaagatc tgctttttgc ttgtactggg
4681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
4741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
4801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
4861 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
4921 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
4981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
5041 aatgtatctt atcatgtctg gcatctatgt cgggtgcgga gaaagaggta atgaaatggc
5101 attatgggta ttatgggtct gcattaatga atcggccaac gatcccggtg tgaaataccg
5161 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
5221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
5281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
5341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
5401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
5461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
5521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
5581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
5641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
5701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
5761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
5821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
5881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
5941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
6001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
6061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
6121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
6181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
6241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
6301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
6361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
6421 gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga
6481 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
6541 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
6601 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
6661 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct
6721 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg
6781 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
6841 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
6901 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
6961 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
7021 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
7081 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
7141 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
7201 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
7261 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
7321 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
7381 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
7441 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
7501 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
7561 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
7621 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca
7681 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
7741 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg
7801 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
7861 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
7921 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc
7981 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca
8041 ataatatacc tt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
SmaI
|
XmaI ClaI
| | |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgc 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACG
3121 aggtgaattcactagaGGATCCTTTTAtgcaccaaaagagaactgcaatgtttcaggacc 3180
TCCACTTAAGTGATCTCCTAGGAAAATACGTGGTTTTCTCTTGACGTTACAAAGTCCTGG
3181 cacaggagcgacccagaaagttaccacagttatgcacagagctgcaaacaactatacatg 3240
GTGTCCTCGCTGGGTCTTTCAATGGTGTCAATACGTGTCTCGACGTTTGTTGATATGTAC
3241 atataatattagaatgtgtgtactgcaagcaacagttactgcgacgtgaggtatatgact 3300
TATATTATAATCTTACACACATGACGTTCGTTGTCAATGACGCTGCACTCCATATACTGA
3301 ttgcttttcgggatttatgcatagtatatagagatgggaatccatatgctgtatgtgata 3360
AACGAAAAGCCCTAAATACGTATCATATATCTCTACCCTTAGGTATACGACATACACTAT
3361 aatgtttaaagttttattctaaaattagtgagtatagacattattgttatagtttgtatg 3420
TTACAAATTTCAAAATAAGATTTTAATCACTCATATCTGTAATAACAATATCAAACATAC
3421 gaacaacattagaacagcaatacaacaaaccgttgtgtgatttgttaattaggtgtatta 3480
CTTGTTGTAATCTTGTCGTTATGTTGTTTGGCAACACACTAAACAATTAATCCACATAAT
3481 actgtcaaaagccactgtgtcctgaagaaaagcaaagacatctggacaaaaagcaaagat 3540
TGACAGTTTTCGGTGACACAGGACTTCTTTTCGTTTCTGTAGACCTGTTTTTCGTTTCTA
3541 tccataatataaggggtcggtggaccggtcgatgtatgtcttgttgcagatcatcaagaa 3600
AGGTATTATATTCCCCAGCCACCTGGCCAGCTACATACAGAACAACGTCTAGTAGTTCTT
3601 cacgtagagaaacccagctgtaatcatgcatggagatacacctacattgcatgaatatat 3660
GTGCATCTCTTTGGGTCGACATTAGTACGTACCTCTATGTGGATGTAACGTACTTATATA
3661 gttagatttgcaaccagagacaactgatctctactgttatgagcaattaaatgacagctc 3720
CAATCTAAACGTTGGTCTCTGTTGACTAGAGATGACAATACTCGTTAATTTACTGTCGAG
3721 agaggaggaggatgaaatagatggtccagctggacaagcagaaccggacagagcccatta 3780
TCTCCTCCTCCTACTTTATCTACCAGGTCGACCTGTTCGTCTTGGCCTGTCTCGGGTAAT
3781 caatattgtaaccttttgttgcaagtgtgactctacgcttcggttgtgcgtacaaagcac 3840
GTTATAACATTGGAAAACAACGTTCACACTGAGATGCGAAGCCAACACGCATGTTTCGTG
3841 acacgtagacattcgtactttggaagacctgttaatgggcacactaggaattgtgtgccc 3900
TGTGCATCTGTAAGCATGAAACCTTCTGGACAATTACCCGTGTGATCCTTAACACACGGG
ORF_2 rf(2)(3929,4636)>>>
|
3901 catctgttctcagaaaccataaTCTACCATGGCTGATcctgcaggtaccggatccggtac 3960
GTAGACAAGAGTCTTTGGTATTAGATGGTACCGACTAGGACGTCCATGGCCTAGGCCATG
NotI
|
ApaI XbaI
| | |
3961 cgagctcgaattcaagcttaagtttatcgggcccgcggccgctggatcctctagactgca 4020
GCTCGAGCTTAAGTTCGAATTCAAATAGCCCGGGCGCCGGCGACCTAGGAGATCTGACGT
XhoI
|
4021 gctcgagaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaacta 4080
CGAGCTCTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGAT
4081 tgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgc 4140
ACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACG
4141 ttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatga 4200
AAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACT
4201 ggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaac 4260
CCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTG
4261 ccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccc 4320
GGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGG
4321 cctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggc 4380
GGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCG
4381 tcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatg 4440
AGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTAC
4441 gctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttc 4500
CGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAG
4501 ggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttcc 4560
CCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGG
4561 gcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtc 4620
CGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAG
delta_U3 other(4625,4677)>>> HIV-1_5_LTR other(4678,4858)>>>
| |
4621 cggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactggg 4680
GCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCC
4681 tctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactg 4740
AGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGAC
4741 cttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgt 4800
GAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACA
4801 gactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagt 4860
CTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCA
4861 agtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagag 4920
TCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTC
4921 agtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcaca 4980
TCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGT
4981 aatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatc 5040
TTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAG
5041 aatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggc 5100
TTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCG
5101 attatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccg 5160
TAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGC
5161 cacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgac 5220
GTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTG
5221 tcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaata 5280
AGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTAT
5281 cggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa 5340
GCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTT
pBR322 origin(5364,5983)<<<
|
5341 aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccct 5400
TTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGA
5401 gacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataa 5460
CTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATT
5461 agataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg 5520
TCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGC
5521 cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctca 5580
GAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGT
5581 cgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaa 5640
GCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTT
5641 ccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccg 5700
GGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGC
5701 gtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgagg 5760
CATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCC
5761 tatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagg 5820
ATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCC
5821 acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagc 5880
TGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCG
5881 tcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcag 5940
AGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTC
5941 attacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgac 6000
TAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTG
6001 gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatc 6060
CGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAG
6061 ttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgag 6120
AAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTC
6121 taaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgt 6180
ATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACA
6181 ctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggag 6240
GATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTC
6241 ggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctcca 6300
CCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGT
6301 gatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaact 6360
CTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGA
6361 ttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgcca 6420
AATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGT
6421 gttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtaga 6480
CAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCT
6481 aagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctgga 6540
TTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCT
6541 caagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgat 6600
GTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTA
NEOKAN prom(6636,6685)>>>
|
6601 agctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccct 6660
TCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGA
6661 ctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatct 6720
GACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGA
NTP_II marker(6777,7565)>>>
|
BclI ORF_3 rf(3)(6774,7568)>>>
| | |
6721 gatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattg 6780
CTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAAC
6781 aacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatg 6840
TTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATAC
6841 actgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcagg 6900
TGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCC
6901 ggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacg 6960
CCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGC
6961 aggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacg 7020
TCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGC
7021 ttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcc 7080
AACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGG
7081 tgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggc 7140
ACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCG
7141 tgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagc 7200
ACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCG
7201 gagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatc 7260
CTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAG
7261 aggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgagg 7320
TCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCC
7321 atctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgct 7380
TAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGA
7381 tttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgt 7440
AAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCA
7441 tggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgc 7500
ACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACG
7501 tttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagt 7560
AAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCA
7561 tcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaa 7620
AGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTT
f1 origin(7673,7978)<<<
|
7621 atcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttcca 7680
TAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGT
7681 gtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaacc 7740
CAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGG
7741 gtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcg 7800
CAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGC
7801 aggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacgg 7860
TCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCC
7861 ggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagg 7920
CCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCC
7921 gcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgc 7980
CGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCG
7981 cgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatca 8040
GCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGT
8041 ataatatacctt 8052
TATTATATGGAA
LOCUS dna 8052 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
ORF 3929..4636
/sequence="ORF_2 rf(2)"
misc_binding 3989..3994
/dbxref="REBASE:ApaI"
misc_binding 3995..4002
/dbxref="REBASE:NotI"
misc_binding 4010..4015
/dbxref="REBASE:XbaI"
misc_binding 4022..4027
/dbxref="REBASE:XhoI"
Other Gene 4625..4677
/gene="delta_U3 other"
Other Gene 4678..4858
/gene="HIV-1_5_LTR other"
Rep_Origin 5364..5983
/gene="pBR322 origin"
Promoter 6636..6685
/gene="NEOKAN prom"
misc_binding 6743..6748
/dbxref="REBASE:BclI"
ORF 6774..7568
/sequence="ORF_3 rf(3)"
Marker 6777..7565
/gene="NTP_II marker"
Rep_Origin 7673..7978
/gene="f1 origin"
BASE COUNT 2158 a 1817 c 2104 g 1973 t 0 others