pLenti-Myc T58A
Vector Name | pLenti-Myc T58A |
VectorType | Lentiviral Vector |
Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
Sequencing Primers | CMV Sequencing Primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggtagcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcgccgctgatcttcagacctggaggaggagatatgagggacaattgagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccggctagcgtttaaacttaagcttggtaccggatccactagtgaattcggcacgaggcggctagggtggaagagccgggcgagcagagctgcgctgcgggcgtcctgggaagggagatccggagcgaatagggggcttcgcctctggcccagccctcccgctgatcccccagccagcggtccgcaacccttgccgcatccacgaaactttgcccatagcagcgggcgggcactttgcactggaacttacaacacccgagcaaggacgcgactctcccgacgcggggaggctattctgcccatttggggacacttccccgccgctgccaggacccgcttctctgaaaggctctccttgcagctgcttagacgctggatttttttcgggtagtggaaaaccagcctcccgcgacgatgcccctcaacgttagcttcaccaacaggaactatgacctcgactacgactcggtgcagccgtatttctactgcgacgaggaggagaacttctaccagcagcagcagcagagcgagctgcagcccccggcgcccagcgaggatatctggaagaaattcgagctgctgcccgctccgcccctgtcccctagccgccgctccgggctctgctcgccctcctacgttgcggtcacacccttctcccttcggggagacaacgacggcggtggcgggagcttctccacggccgaccagctggagatggtgaccgagctgctgggaggagacatggtgaaccagagtttcatctgcgacccggacgacgagaccttcatcaaaaacatcatcatccaggactgtatgtggagcggcttctcggccgccgccaagctcgtctcagagaagctggcctcctaccaggctgcgcgcaaagacagcggcagcccgaaccccgcccgcggccacagcgtctgctccacctccagcttgtacctgcaggatctgagcgccgccgcctcagagtgcatcgacccctcggtggtcttcccctaccctctcaacgacagcagctcgcccaagtcctgcgcctcgcaagactccagcgccttctctccgtcctcggattctctgctctcctcgacggagtcctccccgcagggcagccccgagcccctggtgctccatgaggagacaccgcccaccaccagcagcgactctgaggaggaacaagaagatgaggaagaaatcgatgttgtttctgtggaaaagaggcaggctcctggcaaaaggtcagagtctggatcaccttctgctggaggccacagcaaacctcctcacagcccactggtcctcaagaggtgccacgtctccacacatcagcacaactacgcagcgcctccctccactcggaaggactatcctgctgccaagagggtcaagttggacagtgtcagagtcctgagacagatcagcaacaaccgaaaatgcaccagccccaggtcctcggacaccgaggagaatgtcaagaggcgaacacacaacgtcttggagcgccagaggaggaacgagctaaaacggagcttttttgccctgcgtgaccagatcccggagttggaaaacaatgaaaaggcccccaaggtagttatccttaaaaaagccacagcatacatcctgtccgtccaagcagaggagcaaaagctcatttctgaagaggacttgttgcggaaacgacgagaacagttgaaacacaaacttgaacagctacggaactcttgtgcgtaaggaaaagtaaggaaaacgattccttctaacagaaatgtcctgagcaatcacctatgaacttgtttcaaatgcatgatcaaatgcaacctcacaaccttggctgagtcttgagactgaaagatttagccataatgtaaactgcctcaaattggactttgggcataaaagaacttttttatgcttaccatcttttttttttctttaacagatttgtatttaagaattgtttttaaaaaattttaagatttacacaatgtttctctgtaaatattgccattaaatgtaaataactttaataaaacgtttatagcagttacacagaatttcaatcctagtatatagtacctagtattataggtactataaaccctaattttttttatttaagtacattttgctttttaaagttgatttttttctattgtttttagaaaaaataaaataactggcaaatataaaaaaaaaaaaaaaaaactcgactctagagcggccgcaccgcggagtactgggccccctaggctcgagcatcatcaccatcaccattgatgatccggctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagcgaccagatctaatgcgtgctagcacgcattagatcggtcgctagcgaccagatctaatgcgtgctagcgaccagatctaatgcgtgctaggcttttgcaaaaagcttgggctgcaggtcgaggcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgatatctagcacgcattagatctggtcgctagcgaccagatctaatgcgtgctagcacgcattagatctggtcgctagacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttggggcctgaccagatctaatgcgtaggcctgaccagatctaatgcgtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgttgcccgggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct3061 tggtaccgga tccactagtg aattcggcac gaggcggcta gggtggaaga gccgggcgag3121 cagagctgcg ctgcgggcgt cctgggaagg gagatccgga gcgaataggg ggcttcgcct3181 ctggcccagc cctcccgctg atcccccagc cagcggtccg caacccttgc cgcatccacg3241 aaactttgcc catagcagcg ggcgggcact ttgcactgga acttacaaca cccgagcaag3301 gacgcgactc tcccgacgcg gggaggctat tctgcccatt tggggacact tccccgccgc3361 tgccaggacc cgcttctctg aaaggctctc cttgcagctg cttagacgct ggattttttt3421 cgggtagtgg aaaaccagcc tcccgcgacg atgcccctca acgttagctt caccaacagg3481 aactatgacc tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac3541 ttctaccagc agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg3601 aagaaattcg agctgctgcc cgctccgccc ctgtccccta gccgccgctc cgggctctgc3661 tcgccctcct acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc3721 gggagcttct ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg3781 gtgaaccaga gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc3841 caggactgta tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc3901 tcctaccagg ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc3961 tgctccacct ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac4021 ccctcggtgg tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg4081 caagactcca gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc4141 ccgcagggca gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc4201 gactctgagg aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg4261 caggctcctg gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct4321 cctcacagcc cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca4381 gcgcctccct ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc4441 agagtcctga gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc4501 gaggagaatg tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta4561 aaacggagct tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc4621 cccaaggtag ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag4681 caaaagctca tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa4741 cttgaacagc tacggaactc ttgtgcgtaa ggaaaagtaa ggaaaacgat tccttctaac4801 agaaatgtcc tgagcaatca cctatgaact tgtttcaaat gcatgatcaa atgcaacctc4861 acaaccttgg ctgagtcttg agactgaaag atttagccat aatgtaaact gcctcaaatt4921 ggactttggg cataaaagaa cttttttatg cttaccatct tttttttttc tttaacagat4981 ttgtatttaa gaattgtttt taaaaaattt taagatttac acaatgtttc tctgtaaata5041 ttgccattaa atgtaaataa ctttaataaa acgtttatag cagttacaca gaatttcaat5101 cctagtatat agtacctagt attataggta ctataaaccc taattttttt tatttaagta5161 cattttgctt tttaaagttg atttttttct attgttttta gaaaaaataa aataactggc5221 aaatataaaa aaaaaaaaaa aaaactcgac tctagagcgg ccgcaccgcg gagtactggg5281 ccccctaggc tcgagcatca tcaccatcac cattgatgat ccggctgtgg aatgtgtgtc5341 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc5401 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc5461 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc5521 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt5581 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt5641 ttggaggcct agcgaccaga tctaatgcgt gctagcacgc attagatcgg tcgctagcga5701 ccagatctaa tgcgtgctag cgaccagatc taatgcgtgc taggcttttg caaaaagctt5761 gggctgcagg tcgaggcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg5821 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg5881 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg5941 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg6001 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg6061 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc6121 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc6181 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc6241 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc6301 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg6361 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct6421 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt6481 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc6541 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt6601 tcttctgagc gggactctgg ggttcgatat ctagcacgca ttagatctgg tcgctagcga6661 ccagatctaa tgcgtgctag cacgcattag atctggtcgc tagacaaggc agctgtagat6721 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga6781 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag6841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt6901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt6961 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta7021 taacttgcaa agaaatgaat atcagagagt gagaggaact tggggcctga ccagatctaa7081 tgcgtaggcc tgaccagatc taatgcgttt aaaccgctga tcagcctcga ctgtgccttc7141 tagttgccag ccatctgttg ttgcccgggc gcgatcgctg cccctccccc gtgccttcct7201 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc7261 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg7321 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg7381 cggaaagaac cagcagatcg atctgcatct atgtcgggtg cggagaaaga ggtaatgaaa7441 tggcattatg ggtattatgg gtctgcatta atgaatcggc caacgatccc ggtgtgaaat7501 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac7561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt7621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca7681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc7741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact7801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct7861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag7921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca7981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa8041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc8101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag8161 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg8221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca8281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc8341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag8401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata8461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat8521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg8581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc8641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc8701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc8761 gccagttaat agtttgcgca acgttgttga aaaaggatct tcacctagat ccttttcacg8821 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc8881 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg8941 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg9001 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg9061 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg9121 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc9181 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg9241 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa9301 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc9361 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat9421 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg9481 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc9541 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag9601 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc9661 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc9721 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata9781 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc9841 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac9901 gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc9961 cgaaatcggc aacatccctt ataaatcaaa agaatagacc gcgatagggt tgagtgttgt10021 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa10081 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttgcg10141 gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg agcccccgat ttagagcttg10201 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc10261 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg cgcgcttaat10321 gcgccgctac agggcgcgtc cattcgccat tcaggatcga attaattctt aattaacatc10381 atcaataata tacctt