pLenti-SV40-T-No-Selection
| Vector Name | pLenti-SV40-T-No-Selection |
| VectorType | Lentivector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc actagtaccg gtaggcctgt cgacgatatc gggcccgcgg ccgctggatc
3181 ccgcgaccgg tcgaccagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
3241 gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc ctctgatgag
3301 aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa
3361 aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat atgctcatca
3421 acctgacttt ggaggcttct gggatgcaac tgagattcca acctatggaa ctgatgaatg
3481 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
3541 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
3601 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
3661 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
3721 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
3781 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
3841 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
3901 gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg
3961 gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa
4021 gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat
4081 gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc
4141 cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa
4201 aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga
4261 tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag
4321 gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt
4381 tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg
4441 catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg
4501 taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa
4561 tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt
4621 ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat
4681 taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa
4741 gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta
4801 cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga
4861 ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag
4921 tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag
4981 tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt
5041 gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa
5101 cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga
5161 gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt
5221 tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag
5281 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
5341 atgcaattgt tgttgttaac ggggatcctc tagactgcag ctcgagaatc aacctctgga
5401 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
5461 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
5521 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
5581 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
5641 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
5701 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
5761 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
5821 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
5881 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctcgcct tcgccctcag
5941 acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc ggatggaagg gctaattcac
6001 tcccaacgaa tacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
6181 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
6241 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
6301 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
6361 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
6421 catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg
6481 cattaatgaa tcggccaacg atcccggtgt gaaataccgc acagatgcgt aaggagaaaa
6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
7801 gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt
7861 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
7921 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
7981 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
8041 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct
8101 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
8161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
8221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
8281 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
8341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
8401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
8461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
8521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
8581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
8641 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
8701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
8761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
8821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
8881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat
8941 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa
9001 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta
9061 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
9121 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat
9181 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
9241 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
9301 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc
9361 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t
LOCUS dna 9411 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
misc_binding 3125..3130
/dbxref="REBASE:EcoRI"
misc_binding 3143..3148
/dbxref="REBASE:StuI"
misc_binding 3155..3160
/dbxref="REBASE:EcoRV"
misc_binding 3161..3166
/dbxref="REBASE:ApaI"
misc_binding 3167..3174
/dbxref="REBASE:NotI"
ORF 3209..5335
/sequence="ORF_2 rf(2)"
Other Gene 3467..5335
/gene="SV40_lrgT other"
misc_binding 5369..5374
/dbxref="REBASE:XbaI"
misc_binding 5381..5386
/dbxref="REBASE:XhoI"
Other Gene 5984..6036
/gene="delta_U3 other"
Other Gene 6037..6217
/gene="HIV-1_5_LTR other"
Rep_Origin 6723..7342
/gene="pBR322 origin"
Promoter 7995..8044
/gene="NEOKAN prom"
ORF 8133..8927
/sequence="ORF_3 rf(3)"
Marker 8136..8924
/gene="NTP_II marker"
Rep_Origin 9032..9337
/gene="f1 origin"
BASE COUNT 2614 a 2003 c 2422 g 2372 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc actagtaccg gtaggcctgt cgacgatatc gggcccgcgg ccgctggatc
3181 ccgcgaccgg tcgaccagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
3241 gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc ctctgatgag
3301 aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa
3361 aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat atgctcatca
3421 acctgacttt ggaggcttct gggatgcaac tgagattcca acctatggaa ctgatgaatg
3481 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
3541 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
3601 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
3661 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
3721 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
3781 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
3841 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
3901 gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg
3961 gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa
4021 gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat
4081 gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc
4141 cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa
4201 aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga
4261 tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag
4321 gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt
4381 tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg
4441 catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg
4501 taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa
4561 tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt
4621 ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat
4681 taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa
4741 gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta
4801 cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga
4861 ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag
4921 tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag
4981 tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt
5041 gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa
5101 cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga
5161 gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt
5221 tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag
5281 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
5341 atgcaattgt tgttgttaac ggggatcctc tagactgcag ctcgagaatc aacctctgga
5401 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
5461 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
5521 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
5581 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
5641 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
5701 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
5761 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
5821 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
5881 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctcgcct tcgccctcag
5941 acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc ggatggaagg gctaattcac
6001 tcccaacgaa tacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
6181 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
6241 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
6301 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
6361 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
6421 catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg
6481 cattaatgaa tcggccaacg atcccggtgt gaaataccgc acagatgcgt aaggagaaaa
6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
7801 gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt
7861 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
7921 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
7981 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
8041 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct
8101 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
8161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
8221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
8281 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
8341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
8401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
8461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
8521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
8581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
8641 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
8701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
8761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
8821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
8881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat
8941 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa
9001 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta
9061 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
9121 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat
9181 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
9241 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
9301 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc
9361 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
SmaI
|
XmaI ClaI KpnI
| | | |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgc 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACG
NotI
|
EcoRI StuI EcoRV ApaI
| | | | |
3121 aggtgaattcactagtaccggtaggcctgtcgacgatatcgggcccgcggccgctggatc 3180
TCCACTTAAGTGATCATGGCCATCCGGACAGCTGCTATAGCCCGGGCGCCGGCGACCTAG
ORF_2 rf(2)(3209,5335)>>>
|
3181 cCGCGACCGGTCGACCAGCTTTGCAAAGatggataaagttttaaacagagaggaatcttt 3240
GGCGCTGGCCAGCTGGTCGAAACGTTTCTACCTATTTCAAAATTTGTCTCTCCTTAGAAA
3241 gcagctaatggaccttctaggtcttgaaaggagtgcctgggggaatattcctctgatgag 3300
CGTCGATTACCTGGAAGATCCAGAACTTTCCTCACGGACCCCCTTATAAGGAGACTACTC
3301 aaaggcatatttaaaaaaatgcaaggagtttcatcctgataaaggaggagatgaagaaaa 3360
TTTCCGTATAAATTTTTTTACGTTCCTCAAAGTAGGACTATTTCCTCCTCTACTTCTTTT
3361 aatgaagaaaatgaatactctgtacaagaaaatggaagatggagtaaaatatgctcatca 3420
TTACTTCTTTTACTTATGAGACATGTTCTTTTACCTTCTACCTCATTTTATACGAGTAGT
SV40_lrgT other(3467,5335)>>>
|
3421 acctgactttggaggcttctgggatgcaactgagattccaacctatggaactgatgaatg 3480
TGGACTGAAACCTCCGAAGACCCTACGTTGACTCTAAGGTTGGATACCTTGACTACTTAC
3481 ggagcagtggtggaatgcctttaatgaggaaaacctgttttgctcagaagaaatgccatc 3540
CCTCGTCACCACCTTACGGAAATTACTCCTTTTGGACAAAACGAGTCTTCTTTACGGTAG
3541 tagtgatgatgaggctactgctgactctcaacattctactcctccaaaaaagaagagaaa 3600
ATCACTACTACTCCGATGACGACTGAGAGTTGTAAGATGAGGAGGTTTTTTCTTCTCTTT
3601 ggtagaagaccccaaggactttccttcagaattgctaagttttttgagtcatgctgtgtt 3660
CCATCTTCTGGGGTTCCTGAAAGGAAGTCTTAACGATTCAAAAAACTCAGTACGACACAA
3661 tagtaatagaactcttgcttgctttgctatttacaccacaaaggaaaaagctgcactgct 3720
ATCATTATCTTGAGAACGAACGAAACGATAAATGTGGTGTTTCCTTTTTCGACGTGACGA
3721 atacaagaaaattatggaaaaatattctgtaacctttataagtaggcataacagttataa 3780
TATGTTCTTTTAATACCTTTTTATAAGACATTGGAAATATTCATCCGTATTGTCAATATT
3781 tcataacatactgttttttcttactccacacaggcatagagtgtctgctattaataacta 3840
AGTATTGTATGACAAAAAAGAATGAGGTGTGTCCGTATCTCACAGACGATAATTATTGAT
3841 tgctcaaaaattgtgtacctttagctttttaatttgtaaaggggttaataaggaatattt 3900
ACGAGTTTTTAACACATGGAAATCGAAAAATTAAACATTTCCCCAATTATTCCTTATAAA
3901 gatgtatagtgccttgactagagatccattttctgttattgaggaaagtttgccaggtgg 3960
CTACATATCACGGAACTGATCTCTAGGTAAAAGACAATAACTCCTTTCAAACGGTCCACC
3961 gttaaaggagcatgattttaatccagaagaagcagaggaaactaaacaagtgtcctggaa 4020
CAATTTCCTCGTACTAAAATTAGGTCTTCTTCGTCTCCTTTGATTTGTTCACAGGACCTT
4021 gcttgtaacagagtatgcaatggaaacaaaatgtgatgatgtgttgttattgcttgggat 4080
CGAACATTGTCTCATACGTTACCTTTGTTTTACACTACTACACAACAATAACGAACCCTA
4081 gtacttggaatttcagtacagttttgaaatgtgtttaaaatgtattaaaaaagaacagcc 4140
CATGAACCTTAAAGTCATGTCAAAACTTTACACAAATTTTACATAATTTTTTCTTGTCGG
4141 cagccactataagtaccatgaaaagcattatgcaaatgctgctatatttgctgacagcaa 4200
GTCGGTGATATTCATGGTACTTTTCGTAATACGTTTACGACGATATAAACGACTGTCGTT
4201 aaaccaaaaaaccatatgccaacaggctgttgatactgttttagctaaaaagcgggttga 4260
TTTGGTTTTTTGGTATACGGTTGTCCGACAACTATGACAAAATCGATTTTTCGCCCAACT
4261 tagcctacaattaactagagaacaaatgttaacaaacagatttaatgatcttttggatag 4320
ATCGGATGTTAATTGATCTCTTGTTTACAATTGTTTGTCTAAATTACTAGAAAACCTATC
4321 gatggatataatgtttggttctacaggctctgctgacatagaagaatggatggctggagt 4380
CTACCTATATTACAAACCAAGATGTCCGAGACGACTGTATCTTCTTACCTACCGACCTCA
4381 tgcttggctacactgtttgttgcccaaaatggattcagtggtgtatgactttttaaaatg 4440
ACGAACCGATGTGACAAACAACGGGTTTTACCTAAGTCACCACATACTGAAAAATTTTAC
4441 catggtgtacaacattcctaaaaaaagatactggctgtttaaaggaccaattgatagtgg 4500
GTACCACATGTTGTAAGGATTTTTTTCTATGACCGACAAATTTCCTGGTTAACTATCACC
4501 taaaactacattagcagctgctttgcttgaattatgtggggggaaagctttaaatgttaa 4560
ATTTTGATGTAATCGTCGACGAAACGAACTTAATACACCCCCCTTTCGAAATTTACAATT
4561 tttgcccttggacaggctgaactttgagctaggagtagctattgaccagtttttagtagt 4620
AAACGGGAACCTGTCCGACTTGAAACTCGATCCTCATCGATAACTGGTCAAAAATCATCA
4621 ttttgaggatgtaaagggcactggaggggagtccagagatttgccttcaggtcagggaat 4680
AAAACTCCTACATTTCCCGTGACCTCCCCTCAGGTCTCTAAACGGAAGTCCAGTCCCTTA
4681 taataacctggacaatttaagggattatttggatggcagtgttaaggtaaacttagaaaa 4740
ATTATTGGACCTGTTAAATTCCCTAATAAACCTACCGTCACAATTCCATTTGAATCTTTT
4741 gaaacacctaaataaaagaactcaaatatttccccctggaatagtcaccatgaatgagta 4800
CTTTGTGGATTTATTTTCTTGAGTTTATAAAGGGGGACCTTATCAGTGGTACTTACTCAT
4801 cagtgtgcctaaaacactgcaggccagatttgtaaaacaaatagattttaggcccaaaga 4860
GTCACACGGATTTTGTGACGTCCGGTCTAAACATTTTGTTTATCTAAAATCCGGGTTTCT
4861 ttatttaaagcattgcctggaacgcagtgagtttttgttagaaaagagaataattcaaag 4920
AATAAATTTCGTAACGGACCTTGCGTCACTCAAAAACAATCTTTTCTCTTATTAAGTTTC
4921 tggcattgctttgcttcttatgttaatttggtacagacctgtggctgagtttgctcaaag 4980
ACCGTAACGAAACGAAGAATACAATTAAACCATGTCTGGACACCGACTCAAACGAGTTTC
4981 tattcagagcagaattgtggagtggaaagagagattggacaaagagtttagtttgtcagt 5040
ATAAGTCTCGTCTTAACACCTCACCTTTCTCTCTAACCTGTTTCTCAAATCAAACAGTCA
5041 gtatcaaaaaatgaagtttaatgtggctatgggaattggagttttagattggctaagaaa 5100
CATAGTTTTTTACTTCAAATTACACCGATACCCTTAACCTCAAAATCTAACCGATTCTTT
5101 cagtgatgatgatgatgaagacagccaggaaaatgctgataaaaatgaagatggtgggga 5160
GTCACTACTACTACTACTTCTGTCGGTCCTTTTACGACTATTTTTACTTCTACCACCCCT
5161 gaagaacatggaagactcagggcatgaaacaggcattgattcacagtcccaaggctcatt 5220
CTTCTTGTACCTTCTGAGTCCCGTACTTTGTCCGTAACTAAGTGTCAGGGTTCCGAGTAA
5221 tcaggcccctcagtcctcacagtctgttcatgatcataatcagccataccacatttgtag 5280
AGTCCGGGGAGTCAGGAGTGTCAGACAAGTACTAGTATTAGTCGGTATGGTGTAAACATC
5281 aggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaAATGA 5340
TCCAAAATGAACGAAATTTTTTGGAGGGTGTGGAGGGGGACTTGGACTTTGTATTTTACT
XbaI XhoI
| |
5341 ATGCAATTGTTGTTGTTAACGGggatcctctagactgcagctcgagaatcaacctctgga 5400
TACGTTAACAACAACAATTGCCCCTAGGAGATCTGACGTCGAGCTCTTAGTTGGAGACCT
5401 ttacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatg 5460
AATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATAC
5461 tggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcatttt 5520
ACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAA
5521 ctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcag 5580
GAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTC
5581 gcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgc 5640
CGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACG
5641 caccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcgga 5700
GTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCT
5701 actcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaa 5760
TGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTT
5761 ttccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccac 5820
AAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTG
5821 ctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggacct 5880
GACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGA
5881 tccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcag 5940
AGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTC
delta_U3 other(5984,6036)>>>
|
5941 acgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcac 6000
TGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTG
HIV-1_5_LTR other(6037,6217)>>>
|
6001 tcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatc 6060
AGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAG
6061 tgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttg 6120
ACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAAC
6121 ccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatcc 6180
GGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGG
6181 ctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttatt 6240
GAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAA
6241 attcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttatt 6300
TAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAA
6301 gcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattt 6360
CGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAA
6361 ttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgg 6420
AAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACC
6421 catctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctg 6480
GTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGAC
6481 cattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaa 6540
GTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTT
6541 taccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcgg 6600
ATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCC
6601 ctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggg 6660
GACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCC
6661 gataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaag 6720
CTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTC
pBR322 origin(6723,7342)<<<
|
6721 gccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcga 6780
CGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCT
6781 cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccct 6840
GCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGA
6841 ggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcc 6900
CCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGG
6901 tttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcg 6960
AAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGC
6961 gtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgc 7020
CACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCG
7021 tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcca 7080
ACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGT
7081 ctggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagag 7140
GACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTC
7141 ttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgct 7200
AAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGA
7201 ctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacc 7260
GACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGG
7261 accgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagga 7320
TGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCT
7321 tctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactca 7380
AGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGT
7381 cgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat 7440
GCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTA
7441 taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttac 7500
ATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATG
7501 caatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagtt 7560
GTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAA
7561 gcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagt 7620
CGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCA
7621 gctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccag 7680
CGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTC
7681 ccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct 7740
GGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGA
7741 attaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt 7800
TAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAA
7801 gttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggt 7860
CAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCA
7861 gctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaa 7920
CGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTT
7921 agagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttat 7980
TCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATA
NEOKAN prom(7995,8044)>>>
|
7981 ggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccct 8040
CCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGA
8041 gcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagct 8100
CGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGA
NTP_II marker(8136,8924)>>>
|
ORF_3 rf(3)(8133,8927)>>>
| |
8101 ctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcag 8160
GACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTC
8161 gttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcg 8220
CAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGC
8221 gctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtca 8280
CGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGT
8281 agaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggc 8340
TCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCG
8341 tggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggg 8400
ACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCC
8401 actggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctg 8460
TGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGAC
8461 ccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggcta 8520
GGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGAT
8521 cctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 8580
GGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTC
8581 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaac 8640
GGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTG
8641 tgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcg 8700
ACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGC
8701 atgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtg 8760
TACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACAC
8761 gccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctg 8820
CGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGAC
8821 aagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccg 8880
TTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGC
8881 attcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaat 8940
TAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTA
8941 ttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaa 9000
AAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTT
f1 origin(9032,9337)<<<
|
9001 tcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccacta 9060
AGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGAT
9061 ttaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggccca 9120
AATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGT
9121 ctacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaat 9180
GATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTA
9181 cggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcg 9240
GCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGC
9241 agaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtc 9300
TCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAG
9301 acgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattc 9360
TGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAG
9361 gccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 9411
CGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 9411 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
misc_binding 3125..3130
/dbxref="REBASE:EcoRI"
misc_binding 3143..3148
/dbxref="REBASE:StuI"
misc_binding 3155..3160
/dbxref="REBASE:EcoRV"
misc_binding 3161..3166
/dbxref="REBASE:ApaI"
misc_binding 3167..3174
/dbxref="REBASE:NotI"
ORF 3209..5335
/sequence="ORF_2 rf(2)"
Other Gene 3467..5335
/gene="SV40_lrgT other"
misc_binding 5369..5374
/dbxref="REBASE:XbaI"
misc_binding 5381..5386
/dbxref="REBASE:XhoI"
Other Gene 5984..6036
/gene="delta_U3 other"
Other Gene 6037..6217
/gene="HIV-1_5_LTR other"
Rep_Origin 6723..7342
/gene="pBR322 origin"
Promoter 7995..8044
/gene="NEOKAN prom"
ORF 8133..8927
/sequence="ORF_3 rf(3)"
Marker 8136..8924
/gene="NTP_II marker"
Rep_Origin 9032..9337
/gene="f1 origin"
BASE COUNT 2614 a 2003 c 2422 g 2372 t 0 others