pLenti-UTR-GFP-Blank

                            FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        1831..2135
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2136..2339
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2416..2434
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    2437..2455
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     CDS             2492..3211
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2492 .. 2494 / #05fd14
                        2: 2495 .. 2497 / #05fd14 / 1a
                        3: 2498 .. 3211 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3245..3271
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      3456..3591
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             3592..4191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4204..4791
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4675..4686)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4801..5034
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4854..5034
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5106..5227
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5556..6144)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6556..7350
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
     1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
     1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
     2461 ccccgggatc gatcaattgc cggtcgccac catggtgagc aagggcgagg agctgttcac
     2521 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2581 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2641 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2701 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2761 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2821 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2881 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2941 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3001 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3061 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3121 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3181 cactctcggc atggacgagc tgtacaagta aagtacgtag gtacccatag agctagcagt
     3241 cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg tgtggaaagt
     3301 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3361 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3421 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3481 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3541 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc catgaccgag
     3601 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt acgcaccctc
     3661 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga ccgccacatc
     3721 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     3781 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     3841 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     3901 gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     3961 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     4021 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     4081 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     4141 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttcc
     4201 ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4261 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4321 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4381 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4441 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4501 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4561 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4621 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4681 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4741 ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgtccgga
     4801 tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt actgggtctc
     4861 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4921 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4981 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     5041 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     5101 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5161 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5221 tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga aatggcatta
     5281 tgggtattat gggtctgcat taatgaatcg gccaacggat gtcggtgtga aataccgcac
     5341 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     5401 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5461 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5521 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5581 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5641 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5701 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5761 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5821 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5881 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5941 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6001 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6061 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6121 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6181 cagtggaacg aaaactcacg ttaagggatt ttggccatga gattatcaaa aaggatcttc
     6241 acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     6301 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     6361 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     6421 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     6481 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga
     6541 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6601 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6661 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6721 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6781 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6841 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6901 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6961 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     7021 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     7081 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     7141 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     7201 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     7261 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7321 ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt aaatcagctc
     7381 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     7441 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     7501 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     7561 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     7621 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     7681 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     7741 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggatcgaa
     7801 ttaattctta attaacatca tcaataatat accttttttg gattgaagcc aatatgataa
     7861 tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag
     7921 tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa
     7981 aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta
     8041 ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa
     8101 actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaatactct
     8161 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8221 atttccccga aaagtgccac ctgacgtaac tataacggtc ctaaggtagc gaaaatgtag
     8281 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     8341 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgat
                        

                            FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        1831..2135
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2136..2339
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2416..2434
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    2437..2455
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     CDS             2492..3211
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2492 .. 2494 / #05fd14
                        2: 2495 .. 2497 / #05fd14 / 1a
                        3: 2498 .. 3211 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3245..3271
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      3456..3591
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             3592..4191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4204..4791
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4675..4686)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4801..5034
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4854..5034
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5106..5227
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5556..6144)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6556..7350
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"