pLenti-hTERT-2A-CDK4-Puro

Vector Name	pLenti-hTERT-2A-CDK4-Puro
VectorType	Lentiviral Plasmid
Antibiotic Information	Bacterial: Kanamycin. Mammalian: Puromycin.
Sequencing Primers	CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3`
Additional Information	The gene expression is driven by a CMV promoter, without any Tag or reporter. Vector with Insert: 4422bp Full plasmid size: 12510bp

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
     3061 agctagcGAT ATCAACAAGT TTGTACAAAA AAGTTGGCat gccgcgcgct ccccgctgcc
     3121 gagccgtgcg ctccctgctg cgcagccact accgcgaggt gctgccgctg gccacgttcg
     3181 tgcggcgcct ggggccccag ggctggcggc tggtgcagcg cggggacccg gcggctttcc
     3241 gcgcgctggt ggcccagtgc ctggtgtgcg tgccctggga cgcacggccg ccccccgccg
     3301 ccccctcctt ccgccaggtg tcctgcctga aggagctggt ggcccgagtg ctgcagaggc
     3361 tgtgcgagcg cggcgcgaag aacgtgctgg ccttcggctt cgcgctgctg gacggggccc
     3421 gcgggggccc ccccgaggcc ttcaccacca gcgtgcgcag ctacctgccc aacacggtga
     3481 ccgacgcact gcgggggagc ggggcgtggg ggctgctgct gcgccgcgtg ggcgacgacg
     3541 tgctggttca cctgctggca cgctgcgcgc tctttgtgct ggtggctccc agctgcgcct
     3601 accaggtgtg cgggccgccg ctgtaccagc tcggcgctgc cactcaggcc cggcccccgc
     3661 cacacgctag tggaccccga aggcgtctgg gatgcgaacg ggcctggaac catagcgtca
     3721 gggaggccgg ggtccccctg ggcctgccag ccccgggtgc gaggaggcgc gggggcagtg
     3781 ccagccgaag tctgccgttg cccaagaggc ccaggcgtgg cgctgcccct gagccggagc
     3841 ggacgcccgt tgggcagggg tcctgggccc acccgggcag gacgcgtgga ccgagtgacc
     3901 gtggtttctg tgtggtgtca cctgccagac ccgccgaaga agccacctct ttggagggtg
     3961 cgctctctgg cacgcgccac tcccacccat ccgtgggccg ccagcaccac gcgggccccc
     4021 catccacatc gcggccacca cgtccctggg acacgccttg tcccccggtg tacgccgaga
     4081 ccaagcactt cctctactcc tcaggcgaca aggagcagct gcggccctcc ttcctactca
     4141 gctctctgag gcccagcctg actggcgctc ggaggctcgt ggagaccatc tttctgggtt
     4201 ccaggccctg gatgccaggg actccccgca ggttgccccg cctgccccag cgctactggc
     4261 aaatgcggcc cctgtttctg gagctgcttg ggaaccacgc gcagtgcccc tacggggtgc
     4321 tcctcaagac gcactgcccg ctgcgagctg cggtcacccc agcagccggt gtctgtgccc
     4381 gggagaagcc ccagggctct gtggcggccc ccgaggagga ggacacagac ccccgtcgcc
     4441 tggtgcagct gctccgccag cacagcagcc cctggcaggt gtacggcttc gtgcgggcct
     4501 gcctgcgccg gctggtgccc ccaggcctct ggggctccag gcacaacgaa cgccgcttcc
     4561 tcaggaacac caagaagttc atctccctgg ggaagcatgc caagctctcg ctgcaggagc
     4621 tgacgtggaa gatgagcgtg cgggactgcg cttggctgcg caggagccca ggggttggct
     4681 gtgttccggc cgcagagcac cgtctgcgtg aggagatcct ggccaagttc ctgcactggc
     4741 tgatgagtgt gtacgtcgtc gagctgctca ggtctttctt ttatgtcacg gagaccacgt
     4801 ttcaaaagaa caggctcttt ttctaccgga agagtgtctg gagcaagttg caaagcattg
     4861 gaatcagaca gcacttgaag agggtgcagc tgcgggagct gtcggaagca gaggtcaggc
     4921 agcatcggga agccaggccc gccctgctga cgtccagact ccgcttcatc cccaagcctg
     4981 acgggctgcg gccgattgtg aacatggact acgtcgtggg agccagaacg ttccgcagag
     5041 aaaagagggc cgagcgtctc acctcgaggg tgaaggcact gttcagcgtg ctcaactacg
     5101 agcgggcgcg gcgccccggc ctcctgggcg cctctgtgct gggcctggac gatatccaca
     5161 gggcctggcg caccttcgtg ctgcgtgtgc gggcccagga cccgccgcct gagctgtact
     5221 ttgtcaaggt ggatgtgacg ggcgcgtacg acaccatccc ccaggacagg ctcacggagg
     5281 tcatcgccag catcatcaaa ccccagaaca cgtactgcgt gcgtcggtat gccgtggtcc
     5341 agaaggccgc ccatgggcac gtccgcaagg ccttcaagag ccacgtctct accttgacag
     5401 acctccagcc gtacatgcga cagttcgtgg ctcacctgca ggagaccagc ccgctgaggg
     5461 atgccgtcgt catcgagcag agctcctccc tgaatgaggc cagcagtggc ctcttcgacg
     5521 tcttcctacg cttcatgtgc caccacgccg tgcgcatcag gggcaagtcc tacgtccagt
     5581 gccaggggat cccgcagggc tccatcctct ccacgctgct ctgcagcctg tgctacggcg
     5641 acatggagaa caagctgttt gcggggattc ggcgggacgg gctgctcctg cgtttggtgg
     5701 atgatttctt gttggtgaca cctcacctca cccacgcgaa aaccttcctc aggaccctgg
     5761 tccgaggtgt ccctgagtat ggctgcgtgg tgaacttgcg gaagacagtg gtgaacttcc
     5821 ctgtagaaga cgaggccctg ggtggcacgg cttttgttca gatgccggcc cacggcctat
     5881 tcccctggtg cggcctgctg ctggataccc ggaccctgga ggtgcagagc gactactcca
     5941 gctatgcccg gacctccatc agagccagtc tcaccttcaa ccgcggcttc aaggctggga
     6001 ggaacatgcg tcgcaaactc tttggggtct tgcggctgaa gtgtcacagc ctgtttctgg
     6061 atttgcaggt gaacagcctc cagacggtgt gcaccaacat ctacaagatc ctcctgctgc
     6121 aggcgtacag gtttcacgca tgtgtgctgc agctcccatt tcatcagcaa gtttggaaga
     6181 accccacatt tttcctgcgc gtcatctctg acacggcctc cctctgctac tccatcctga
     6241 aagccaagaa cgcagggatg tcgctggggg ccaagggcgc cgccggccct ctgccctccg
     6301 aggccgtgca gtggctgtgc caccaagcat tcctgctcaa gctgactcga caccgtgtca
     6361 cctacgtgcc actcctgggg tcactcagga cagcccagac gcagctgagt cggaagctcc
     6421 cggggacgac gctgactgcc ctggaggccg cagccaaccc ggcactgccc tcagacttca
     6481 agaccatcct ggactgCCCA ACTTTCTTGT ACAAAGTGGT Tgatatcggg cccgcggccg
     6541 ctggatcctc tagcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     6601 ccggccctAT GGCTACCTCT CGATATGAGC CAGTGGCTGA AATTGGTGTC GGTGCCTATG
     6661 GGACAGTGTA CAAGGCCCGT GATCCCCACA GTGGCCACTT TGTGGCCCTC AAGAGTGTGA
     6721 GAGTCCCCAA TGGAGGAGGA GGTGGAGGAG GCCTTCCCAT CAGCACAGTT CGTGAGGTGG
     6781 CTTTACTGAG GCGACTGGAG GCTTTTGAGC ATCCCAATGT TGTCCGGCTG ATGGACGTCT
     6841 GTGCCACATC CCGAACTGAC CGGGAGATCA AGGTAACCCT GGTGTTTGAG CATGTAGACC
     6901 AGGACCTAAG GACATATCTG GACAAGGCAC CCCCACCAGG CTTGCCAGCC GAAACGATCA
     6961 AGGATCTGAT GCGCCAGTTT CTAAGAGGCC TAGATTTCCT TCATGCCAAT TGCATCGTTC
     7021 ACCGAGATCT GAAGCCAGAG AACATTCTGG TGACAAGTGG TGGAACAGTC AAGCTGGCTG
     7081 ACTTTGGCCT GGCCAGAATC TACAGCTACC AGATGGCACT TACACCCGTG GTTGTTACAC
     7141 TCTGGTACCG AGCTCCCGAA GTTCTTCTGC AGTCCACATA TGCAACACCT GTGGACATGT
     7201 GGAGTGTTGG CTGTATCTTT GCAGAGATGT TTCGTCGAAA GCCTCTCTTC TGTGGAAACT
     7261 CTGAAGCCGA CCAGTTGGGC AAAATCTTTG ACCTGATTGG GCTGCCTCCA GAGGATGACT
     7321 GGCCTCGAGA TGTATCCCTG CCCCGTGGAG CCTTTCCCCC CAGAGGGCCC CGCCCAGTGC
     7381 AGTCGGTGGT ACCTGAGATG GAGGAGTCGG GAGCACAGCT GCTGCTGGAA ATGCTGACTT
     7441 TTAACCCACA CAAGCGAATC TCTGCCTTTC GAGCTCTGCA GCACTCTTAT CTACATAAGG
     7501 ATGAAGGTAA TCCGGAGTGA ctcgagtacc catacgacgt cccagactac gcttgagttt
     7561 aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7621 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7681 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7741 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7801 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7861 ttttttggag gccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     7921 ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7981 cgtggacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     8101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaagggctcc tggcgccgca
     8221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     8281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     8341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     8401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     8461 gcccggtgcc tgaacgcgtt ccggaaatca acctctggat tacaaaattt gtgaaagatt
     8521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     8581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     8641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     8701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     8761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     8821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     8881 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     8941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9001 ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga cgagtcggat ctccctttgg
     9061 gccgcctccc cgcctgtccg gatggaaggg ctaattcact cccaacgaat acaagatctg
     9121 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9301 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     9361 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     9421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9481 ggtttgtcca aactcatcaa tgtatcttat catgtctggc atctatgtcg ggtgcggaga
     9541 aagaggtaat gaaatggcat tatgggtatt atgggtctgc attaatgaat cggccaacga
     9601 tcccggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     9661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10261 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgaaaaagg atcttcacct
    10921 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
    10981 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
    11041 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
    11101 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
    11161 tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
    11221 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
    11281 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
    11341 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
    11401 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
    11461 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
    11521 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
    11581 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
    11641 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    11701 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
    11761 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    11821 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    11881 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    11941 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    12001 atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat cagctcattt
    12061 tttaaccaat aggccgaaat cggcaacatc ccttataaat caaaagaata gaccgcgata
    12121 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    12181 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa
    12241 tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc
    12301 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    12361 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    12421 cccgcgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat
    12481 tcttaattaa catcatcaat aatatacctt

                            LOCUS       dna                     12510 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     Promoter        2885..2966
                     /gene="CMV prom"
     ORF             2901..7520
                     /sequence="ORF_3 rf(3)"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     ORF             3072..3713
                     /sequence="ORF_6 rf(5)"
     ORF             4134..5354
                     /sequence="ORF_5 rf(5)"
     misc_binding    6534..6541
                     /dbxref="REBASE:NotI"
     Promoter        7571..7839
                     /gene="SV40 prom"
     Rep_Origin      7738..7815
                     /gene="SV40 origin"
     ORF             7805..8473
                     /sequence="ORF_2 rf(2)"
     misc_binding    7818..7830
                     /dbxref="REBASE:SfiI"
     Marker          7874..8473
                     /gene="puro marker"
     Other Gene      9083..9135
                     /gene="delta_U3 other"
     Other Gene      9136..9316
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      9822..10441
                     /gene="pBR322 origin"
     Promoter        11094..11143
                     /gene="NEOKAN prom"
     misc_binding    11201..11206
                     /dbxref="REBASE:BclI"
     ORF             11232..12026
                     /sequence="ORF_4 rf(3)"
     Marker          11235..12023
                     /gene="NTP_II marker"
     Rep_Origin      12131..12436
                     /gene="f1 origin"
BASE COUNT    2823 a   3408 c   3559 g   2720 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gccgcgcgct ccccgctgcc     
     3121 gagccgtgcg ctccctgctg cgcagccact accgcgaggt gctgccgctg gccacgttcg     
     3181 tgcggcgcct ggggccccag ggctggcggc tggtgcagcg cggggacccg gcggctttcc     
     3241 gcgcgctggt ggcccagtgc ctggtgtgcg tgccctggga cgcacggccg ccccccgccg     
     3301 ccccctcctt ccgccaggtg tcctgcctga aggagctggt ggcccgagtg ctgcagaggc     
     3361 tgtgcgagcg cggcgcgaag aacgtgctgg ccttcggctt cgcgctgctg gacggggccc     
     3421 gcgggggccc ccccgaggcc ttcaccacca gcgtgcgcag ctacctgccc aacacggtga     
     3481 ccgacgcact gcgggggagc ggggcgtggg ggctgctgct gcgccgcgtg ggcgacgacg     
     3541 tgctggttca cctgctggca cgctgcgcgc tctttgtgct ggtggctccc agctgcgcct     
     3601 accaggtgtg cgggccgccg ctgtaccagc tcggcgctgc cactcaggcc cggcccccgc     
     3661 cacacgctag tggaccccga aggcgtctgg gatgcgaacg ggcctggaac catagcgtca     
     3721 gggaggccgg ggtccccctg ggcctgccag ccccgggtgc gaggaggcgc gggggcagtg     
     3781 ccagccgaag tctgccgttg cccaagaggc ccaggcgtgg cgctgcccct gagccggagc     
     3841 ggacgcccgt tgggcagggg tcctgggccc acccgggcag gacgcgtgga ccgagtgacc     
     3901 gtggtttctg tgtggtgtca cctgccagac ccgccgaaga agccacctct ttggagggtg     
     3961 cgctctctgg cacgcgccac tcccacccat ccgtgggccg ccagcaccac gcgggccccc     
     4021 catccacatc gcggccacca cgtccctggg acacgccttg tcccccggtg tacgccgaga     
     4081 ccaagcactt cctctactcc tcaggcgaca aggagcagct gcggccctcc ttcctactca     
     4141 gctctctgag gcccagcctg actggcgctc ggaggctcgt ggagaccatc tttctgggtt     
     4201 ccaggccctg gatgccaggg actccccgca ggttgccccg cctgccccag cgctactggc     
     4261 aaatgcggcc cctgtttctg gagctgcttg ggaaccacgc gcagtgcccc tacggggtgc     
     4321 tcctcaagac gcactgcccg ctgcgagctg cggtcacccc agcagccggt gtctgtgccc     
     4381 gggagaagcc ccagggctct gtggcggccc ccgaggagga ggacacagac ccccgtcgcc     
     4441 tggtgcagct gctccgccag cacagcagcc cctggcaggt gtacggcttc gtgcgggcct     
     4501 gcctgcgccg gctggtgccc ccaggcctct ggggctccag gcacaacgaa cgccgcttcc     
     4561 tcaggaacac caagaagttc atctccctgg ggaagcatgc caagctctcg ctgcaggagc     
     4621 tgacgtggaa gatgagcgtg cgggactgcg cttggctgcg caggagccca ggggttggct     
     4681 gtgttccggc cgcagagcac cgtctgcgtg aggagatcct ggccaagttc ctgcactggc     
     4741 tgatgagtgt gtacgtcgtc gagctgctca ggtctttctt ttatgtcacg gagaccacgt     
     4801 ttcaaaagaa caggctcttt ttctaccgga agagtgtctg gagcaagttg caaagcattg     
     4861 gaatcagaca gcacttgaag agggtgcagc tgcgggagct gtcggaagca gaggtcaggc     
     4921 agcatcggga agccaggccc gccctgctga cgtccagact ccgcttcatc cccaagcctg     
     4981 acgggctgcg gccgattgtg aacatggact acgtcgtggg agccagaacg ttccgcagag     
     5041 aaaagagggc cgagcgtctc acctcgaggg tgaaggcact gttcagcgtg ctcaactacg     
     5101 agcgggcgcg gcgccccggc ctcctgggcg cctctgtgct gggcctggac gatatccaca     
     5161 gggcctggcg caccttcgtg ctgcgtgtgc gggcccagga cccgccgcct gagctgtact     
     5221 ttgtcaaggt ggatgtgacg ggcgcgtacg acaccatccc ccaggacagg ctcacggagg     
     5281 tcatcgccag catcatcaaa ccccagaaca cgtactgcgt gcgtcggtat gccgtggtcc     
     5341 agaaggccgc ccatgggcac gtccgcaagg ccttcaagag ccacgtctct accttgacag     
     5401 acctccagcc gtacatgcga cagttcgtgg ctcacctgca ggagaccagc ccgctgaggg     
     5461 atgccgtcgt catcgagcag agctcctccc tgaatgaggc cagcagtggc ctcttcgacg     
     5521 tcttcctacg cttcatgtgc caccacgccg tgcgcatcag gggcaagtcc tacgtccagt     
     5581 gccaggggat cccgcagggc tccatcctct ccacgctgct ctgcagcctg tgctacggcg     
     5641 acatggagaa caagctgttt gcggggattc ggcgggacgg gctgctcctg cgtttggtgg     
     5701 atgatttctt gttggtgaca cctcacctca cccacgcgaa aaccttcctc aggaccctgg     
     5761 tccgaggtgt ccctgagtat ggctgcgtgg tgaacttgcg gaagacagtg gtgaacttcc     
     5821 ctgtagaaga cgaggccctg ggtggcacgg cttttgttca gatgccggcc cacggcctat     
     5881 tcccctggtg cggcctgctg ctggataccc ggaccctgga ggtgcagagc gactactcca     
     5941 gctatgcccg gacctccatc agagccagtc tcaccttcaa ccgcggcttc aaggctggga     
     6001 ggaacatgcg tcgcaaactc tttggggtct tgcggctgaa gtgtcacagc ctgtttctgg     
     6061 atttgcaggt gaacagcctc cagacggtgt gcaccaacat ctacaagatc ctcctgctgc     
     6121 aggcgtacag gtttcacgca tgtgtgctgc agctcccatt tcatcagcaa gtttggaaga     
     6181 accccacatt tttcctgcgc gtcatctctg acacggcctc cctctgctac tccatcctga     
     6241 aagccaagaa cgcagggatg tcgctggggg ccaagggcgc cgccggccct ctgccctccg     
     6301 aggccgtgca gtggctgtgc caccaagcat tcctgctcaa gctgactcga caccgtgtca     
     6361 cctacgtgcc actcctgggg tcactcagga cagcccagac gcagctgagt cggaagctcc     
     6421 cggggacgac gctgactgcc ctggaggccg cagccaaccc ggcactgccc tcagacttca     
     6481 agaccatcct ggactgccca actttcttgt acaaagtggt tgatatcggg cccgcggccg     
     6541 ctggatcctc tagcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc     
     6601 ccggccctat ggctacctct cgatatgagc cagtggctga aattggtgtc ggtgcctatg     
     6661 ggacagtgta caaggcccgt gatccccaca gtggccactt tgtggccctc aagagtgtga     
     6721 gagtccccaa tggaggagga ggtggaggag gccttcccat cagcacagtt cgtgaggtgg     
     6781 ctttactgag gcgactggag gcttttgagc atcccaatgt tgtccggctg atggacgtct     
     6841 gtgccacatc ccgaactgac cgggagatca aggtaaccct ggtgtttgag catgtagacc     
     6901 aggacctaag gacatatctg gacaaggcac ccccaccagg cttgccagcc gaaacgatca     
     6961 aggatctgat gcgccagttt ctaagaggcc tagatttcct tcatgccaat tgcatcgttc     
     7021 accgagatct gaagccagag aacattctgg tgacaagtgg tggaacagtc aagctggctg     
     7081 actttggcct ggccagaatc tacagctacc agatggcact tacacccgtg gttgttacac     
     7141 tctggtaccg agctcccgaa gttcttctgc agtccacata tgcaacacct gtggacatgt     
     7201 ggagtgttgg ctgtatcttt gcagagatgt ttcgtcgaaa gcctctcttc tgtggaaact     
     7261 ctgaagccga ccagttgggc aaaatctttg acctgattgg gctgcctcca gaggatgact     
     7321 ggcctcgaga tgtatccctg ccccgtggag cctttccccc cagagggccc cgcccagtgc     
     7381 agtcggtggt acctgagatg gaggagtcgg gagcacagct gctgctggaa atgctgactt     
     7441 ttaacccaca caagcgaatc tctgcctttc gagctctgca gcactcttat ctacataagg     
     7501 atgaaggtaa tccggagtga ctcgagtacc catacgacgt cccagactac gcttgagttt     
     7561 aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg     
     7621 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt     
     7681 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc     
     7741 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt     
     7801 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc     
     7861 ttttttggag gccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt     
     7921 ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac     
     7981 cgtggacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg     
     8041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg     
     8101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc     
     8161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaagggctcc tggcgccgca     
     8221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg     
     8281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt     
     8341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt     
     8401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa     
     8461 gcccggtgcc tgaacgcgtt ccggaaatca acctctggat tacaaaattt gtgaaagatt     
     8521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc     
     8581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg     
     8641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac     
     8701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc     
     8761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc     
     8821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa     
     8881 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc     
     8941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc     
     9001 ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga cgagtcggat ctccctttgg     
     9061 gccgcctccc cgcctgtccg gatggaaggg ctaattcact cccaacgaat acaagatctg     
     9121 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc     
     9181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg     
     9241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg     
     9301 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca     
     9361 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa     
     9421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     
     9481 ggtttgtcca aactcatcaa tgtatcttat catgtctggc atctatgtcg ggtgcggaga     
     9541 aagaggtaat gaaatggcat tatgggtatt atgggtctgc attaatgaat cggccaacga     
     9601 tcccggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc     
     9661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct     
     9721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg     
     9781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc     
     9841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga     
     9901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     
     9961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     
    10021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     
    10081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     
    10141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     
    10201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     
    10261 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc     
    10321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt     
    10381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc     
    10441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg     
    10501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca     
    10561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca     
    10621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag     
    10681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac     
    10741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc     
    10801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct     
    10861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgaaaaagg atcttcacct     
    10921 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca     
    10981 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca     
    11041 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat     
    11101 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt     
    11161 tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag     
    11221 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg     
    11281 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt     
    11341 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc     
    11401 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt     
    11461 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag     
    11521 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg     
    11581 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag     
    11641 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg     
    11701 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga     
    11761 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca     
    11821 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc     
    11881 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg     
    11941 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct     
    12001 atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat cagctcattt     
    12061 tttaaccaat aggccgaaat cggcaacatc ccttataaat caaaagaata gaccgcgata     
    12121 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac     
    12181 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa     
    12241 tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc     
    12301 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg     
    12361 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca     
    12421 cccgcgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat     
    12481 tcttaattaa catcatcaat aatatacctt                                      
//

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1     ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
      AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA

61    gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
      CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT

                                               Encap other(162,310)>>> 
                                               |
121   cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
      GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC

181   aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
      TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA

241   aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
      TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC

301   tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
      ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC

          amp prom(365,393)<<< 
          |
361   ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
      CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC

                                             HincII 
                                             |
                                             HpaI 
                                             |
421   gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
      CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT

481   gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
      CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC

541   caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
      GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC

601   tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
      ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA

                                                            HIV-1_5_LTR other(715,895)>>> 
                                                            |
661   gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
      CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA

721   ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
      GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA

781   aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
      TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG

841   tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
      AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG

901   gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
      CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG

                                                   HIV-1_psi_pack other(1006,1050)>>> 
                                                   |
961   ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
      CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT

1021  ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
      AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC

                    NruI 
                    |
1081  ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
      CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT

1141  aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
      TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG

1201  tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
      ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT

1261  caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
      GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG

1321  aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
      TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT

1381  aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
      TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC

1441  ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
      CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT

                                                              RRE reg(1557,1790)>>> 
                                                              |
1501  gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
      CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT

                                             ORF_1 rf(1)(1600,2301)>>> 
                                             |
1561  gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
      CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC

1621  ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
      GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC

1681  agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
      TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG

1741  caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
      GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC

1801  ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
      CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA

1861  aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
      TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG

1921  aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
      TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA

1981  gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
      CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT

2041  aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
      TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT

2101  atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
      TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC

2161  tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
      AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT

2221  ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
      CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT

2281  aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
      TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC

2341  aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
      TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT

2401  aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
      TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA

2461  ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
      CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA

2521  cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
      GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT

2581  actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
      TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG

2641  aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
      TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA

2701  acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
      TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA

2761  acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
      TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC

2821  acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
      TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT

                          ORF_3 rf(3)(2901,7520)>>> 
                          |
          CMV prom(2885,2966)>>> 
          |               |
2881  actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
      TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT

2941  gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
      CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG

                           tetO reg(3022,3061)>>> 
                           |
3001  atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
      TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC

                 ORF_6 rf(5)(3072,3713)<<< 
                 |
3061  agctagcGATATCAACAAGTTTGTACAAAAAAGTTGGCatgccgcgcgctccccgctgcc 3120
      TCGATCGCTATAGTTGTTCAAACATGTTTTTTCAACCGTACGGCGCGCGAGGGGCGACGG

3121  gagccgtgcgctccctgctgcgcagccactaccgcgaggtgctgccgctggccacgttcg 3180
      CTCGGCACGCGAGGGACGACGCGTCGGTGATGGCGCTCCACGACGGCGACCGGTGCAAGC

3181  tgcggcgcctggggccccagggctggcggctggtgcagcgcggggacccggcggctttcc 3240
      ACGCCGCGGACCCCGGGGTCCCGACCGCCGACCACGTCGCGCCCCTGGGCCGCCGAAAGG

3241  gcgcgctggtggcccagtgcctggtgtgcgtgccctgggacgcacggccgccccccgccg 3300
      CGCGCGACCACCGGGTCACGGACCACACGCACGGGACCCTGCGTGCCGGCGGGGGGCGGC

3301  ccccctccttccgccaggtgtcctgcctgaaggagctggtggcccgagtgctgcagaggc 3360
      GGGGGAGGAAGGCGGTCCACAGGACGGACTTCCTCGACCACCGGGCTCACGACGTCTCCG

3361  tgtgcgagcgcggcgcgaagaacgtgctggccttcggcttcgcgctgctggacggggccc 3420
      ACACGCTCGCGCCGCGCTTCTTGCACGACCGGAAGCCGAAGCGCGACGACCTGCCCCGGG

3421  gcgggggcccccccgaggccttcaccaccagcgtgcgcagctacctgcccaacacggtga 3480
      CGCCCCCGGGGGGGCTCCGGAAGTGGTGGTCGCACGCGTCGATGGACGGGTTGTGCCACT

3481  ccgacgcactgcgggggagcggggcgtgggggctgctgctgcgccgcgtgggcgacgacg 3540
      GGCTGCGTGACGCCCCCTCGCCCCGCACCCCCGACGACGACGCGGCGCACCCGCTGCTGC

3541  tgctggttcacctgctggcacgctgcgcgctctttgtgctggtggctcccagctgcgcct 3600
      ACGACCAAGTGGACGACCGTGCGACGCGCGAGAAACACGACCACCGAGGGTCGACGCGGA

3601  accaggtgtgcgggccgccgctgtaccagctcggcgctgccactcaggcccggcccccgc 3660
      TGGTCCACACGCCCGGCGGCGACATGGTCGAGCCGCGACGGTGAGTCCGGGCCGGGGGCG

3661  cacacgctagtggaccccgaaggcgtctgggatgcgaacgggcctggaaccatagcgtca 3720
      GTGTGCGATCACCTGGGGCTTCCGCAGACCCTACGCTTGCCCGGACCTTGGTATCGCAGT

3721  gggaggccggggtccccctgggcctgccagccccgggtgcgaggaggcgcgggggcagtg 3780
      CCCTCCGGCCCCAGGGGGACCCGGACGGTCGGGGCCCACGCTCCTCCGCGCCCCCGTCAC

3781  ccagccgaagtctgccgttgcccaagaggcccaggcgtggcgctgcccctgagccggagc 3840
      GGTCGGCTTCAGACGGCAACGGGTTCTCCGGGTCCGCACCGCGACGGGGACTCGGCCTCG

3841  ggacgcccgttgggcaggggtcctgggcccacccgggcaggacgcgtggaccgagtgacc 3900
      CCTGCGGGCAACCCGTCCCCAGGACCCGGGTGGGCCCGTCCTGCGCACCTGGCTCACTGG

3901  gtggtttctgtgtggtgtcacctgccagacccgccgaagaagccacctctttggagggtg 3960
      CACCAAAGACACACCACAGTGGACGGTCTGGGCGGCTTCTTCGGTGGAGAAACCTCCCAC

3961  cgctctctggcacgcgccactcccacccatccgtgggccgccagcaccacgcgggccccc 4020
      GCGAGAGACCGTGCGCGGTGAGGGTGGGTAGGCACCCGGCGGTCGTGGTGCGCCCGGGGG

4021  catccacatcgcggccaccacgtccctgggacacgccttgtcccccggtgtacgccgaga 4080
      GTAGGTGTAGCGCCGGTGGTGCAGGGACCCTGTGCGGAACAGGGGGCCACATGCGGCTCT

                                                           ORF_5 rf(5)(4134,5354)<<< 
                                                           |
4081  ccaagcacttcctctactcctcaggcgacaaggagcagctgcggccctccttcctactca 4140
      GGTTCGTGAAGGAGATGAGGAGTCCGCTGTTCCTCGTCGACGCCGGGAGGAAGGATGAGT

4141  gctctctgaggcccagcctgactggcgctcggaggctcgtggagaccatctttctgggtt 4200
      CGAGAGACTCCGGGTCGGACTGACCGCGAGCCTCCGAGCACCTCTGGTAGAAAGACCCAA

4201  ccaggccctggatgccagggactccccgcaggttgccccgcctgccccagcgctactggc 4260
      GGTCCGGGACCTACGGTCCCTGAGGGGCGTCCAACGGGGCGGACGGGGTCGCGATGACCG

4261  aaatgcggcccctgtttctggagctgcttgggaaccacgcgcagtgcccctacggggtgc 4320
      TTTACGCCGGGGACAAAGACCTCGACGAACCCTTGGTGCGCGTCACGGGGATGCCCCACG

4321  tcctcaagacgcactgcccgctgcgagctgcggtcaccccagcagccggtgtctgtgccc 4380
      AGGAGTTCTGCGTGACGGGCGACGCTCGACGCCAGTGGGGTCGTCGGCCACAGACACGGG

4381  gggagaagccccagggctctgtggcggcccccgaggaggaggacacagacccccgtcgcc 4440
      CCCTCTTCGGGGTCCCGAGACACCGCCGGGGGCTCCTCCTCCTGTGTCTGGGGGCAGCGG

4441  tggtgcagctgctccgccagcacagcagcccctggcaggtgtacggcttcgtgcgggcct 4500
      ACCACGTCGACGAGGCGGTCGTGTCGTCGGGGACCGTCCACATGCCGAAGCACGCCCGGA

4501  gcctgcgccggctggtgcccccaggcctctggggctccaggcacaacgaacgccgcttcc 4560
      CGGACGCGGCCGACCACGGGGGTCCGGAGACCCCGAGGTCCGTGTTGCTTGCGGCGAAGG

4561  tcaggaacaccaagaagttcatctccctggggaagcatgccaagctctcgctgcaggagc 4620
      AGTCCTTGTGGTTCTTCAAGTAGAGGGACCCCTTCGTACGGTTCGAGAGCGACGTCCTCG

4621  tgacgtggaagatgagcgtgcgggactgcgcttggctgcgcaggagcccaggggttggct 4680
      ACTGCACCTTCTACTCGCACGCCCTGACGCGAACCGACGCGTCCTCGGGTCCCCAACCGA

4681  gtgttccggccgcagagcaccgtctgcgtgaggagatcctggccaagttcctgcactggc 4740
      CACAAGGCCGGCGTCTCGTGGCAGACGCACTCCTCTAGGACCGGTTCAAGGACGTGACCG

4741  tgatgagtgtgtacgtcgtcgagctgctcaggtctttcttttatgtcacggagaccacgt 4800
      ACTACTCACACATGCAGCAGCTCGACGAGTCCAGAAAGAAAATACAGTGCCTCTGGTGCA

4801  ttcaaaagaacaggctctttttctaccggaagagtgtctggagcaagttgcaaagcattg 4860
      AAGTTTTCTTGTCCGAGAAAAAGATGGCCTTCTCACAGACCTCGTTCAACGTTTCGTAAC

4861  gaatcagacagcacttgaagagggtgcagctgcgggagctgtcggaagcagaggtcaggc 4920
      CTTAGTCTGTCGTGAACTTCTCCCACGTCGACGCCCTCGACAGCCTTCGTCTCCAGTCCG

4921  agcatcgggaagccaggcccgccctgctgacgtccagactccgcttcatccccaagcctg 4980
      TCGTAGCCCTTCGGTCCGGGCGGGACGACTGCAGGTCTGAGGCGAAGTAGGGGTTCGGAC

4981  acgggctgcggccgattgtgaacatggactacgtcgtgggagccagaacgttccgcagag 5040
      TGCCCGACGCCGGCTAACACTTGTACCTGATGCAGCACCCTCGGTCTTGCAAGGCGTCTC

5041  aaaagagggccgagcgtctcacctcgagggtgaaggcactgttcagcgtgctcaactacg 5100
      TTTTCTCCCGGCTCGCAGAGTGGAGCTCCCACTTCCGTGACAAGTCGCACGAGTTGATGC

5101  agcgggcgcggcgccccggcctcctgggcgcctctgtgctgggcctggacgatatccaca 5160
      TCGCCCGCGCCGCGGGGCCGGAGGACCCGCGGAGACACGACCCGGACCTGCTATAGGTGT

5161  gggcctggcgcaccttcgtgctgcgtgtgcgggcccaggacccgccgcctgagctgtact 5220
      CCCGGACCGCGTGGAAGCACGACGCACACGCCCGGGTCCTGGGCGGCGGACTCGACATGA

5221  ttgtcaaggtggatgtgacgggcgcgtacgacaccatcccccaggacaggctcacggagg 5280
      AACAGTTCCACCTACACTGCCCGCGCATGCTGTGGTAGGGGGTCCTGTCCGAGTGCCTCC

5281  tcatcgccagcatcatcaaaccccagaacacgtactgcgtgcgtcggtatgccgtggtcc 5340
      AGTAGCGGTCGTAGTAGTTTGGGGTCTTGTGCATGACGCACGCAGCCATACGGCACCAGG

5341  agaaggccgcccatgggcacgtccgcaaggccttcaagagccacgtctctaccttgacag 5400
      TCTTCCGGCGGGTACCCGTGCAGGCGTTCCGGAAGTTCTCGGTGCAGAGATGGAACTGTC

5401  acctccagccgtacatgcgacagttcgtggctcacctgcaggagaccagcccgctgaggg 5460
      TGGAGGTCGGCATGTACGCTGTCAAGCACCGAGTGGACGTCCTCTGGTCGGGCGACTCCC

5461  atgccgtcgtcatcgagcagagctcctccctgaatgaggccagcagtggcctcttcgacg 5520
      TACGGCAGCAGTAGCTCGTCTCGAGGAGGGACTTACTCCGGTCGTCACCGGAGAAGCTGC

5521  tcttcctacgcttcatgtgccaccacgccgtgcgcatcaggggcaagtcctacgtccagt 5580
      AGAAGGATGCGAAGTACACGGTGGTGCGGCACGCGTAGTCCCCGTTCAGGATGCAGGTCA

5581  gccaggggatcccgcagggctccatcctctccacgctgctctgcagcctgtgctacggcg 5640
      CGGTCCCCTAGGGCGTCCCGAGGTAGGAGAGGTGCGACGAGACGTCGGACACGATGCCGC

5641  acatggagaacaagctgtttgcggggattcggcgggacgggctgctcctgcgtttggtgg 5700
      TGTACCTCTTGTTCGACAAACGCCCCTAAGCCGCCCTGCCCGACGAGGACGCAAACCACC

5701  atgatttcttgttggtgacacctcacctcacccacgcgaaaaccttcctcaggaccctgg 5760
      TACTAAAGAACAACCACTGTGGAGTGGAGTGGGTGCGCTTTTGGAAGGAGTCCTGGGACC

5761  tccgaggtgtccctgagtatggctgcgtggtgaacttgcggaagacagtggtgaacttcc 5820
      AGGCTCCACAGGGACTCATACCGACGCACCACTTGAACGCCTTCTGTCACCACTTGAAGG

5821  ctgtagaagacgaggccctgggtggcacggcttttgttcagatgccggcccacggcctat 5880
      GACATCTTCTGCTCCGGGACCCACCGTGCCGAAAACAAGTCTACGGCCGGGTGCCGGATA

5881  tcccctggtgcggcctgctgctggatacccggaccctggaggtgcagagcgactactcca 5940
      AGGGGACCACGCCGGACGACGACCTATGGGCCTGGGACCTCCACGTCTCGCTGATGAGGT

5941  gctatgcccggacctccatcagagccagtctcaccttcaaccgcggcttcaaggctggga 6000
      CGATACGGGCCTGGAGGTAGTCTCGGTCAGAGTGGAAGTTGGCGCCGAAGTTCCGACCCT

6001  ggaacatgcgtcgcaaactctttggggtcttgcggctgaagtgtcacagcctgtttctgg 6060
      CCTTGTACGCAGCGTTTGAGAAACCCCAGAACGCCGACTTCACAGTGTCGGACAAAGACC

6061  atttgcaggtgaacagcctccagacggtgtgcaccaacatctacaagatcctcctgctgc 6120
      TAAACGTCCACTTGTCGGAGGTCTGCCACACGTGGTTGTAGATGTTCTAGGAGGACGACG

6121  aggcgtacaggtttcacgcatgtgtgctgcagctcccatttcatcagcaagtttggaaga 6180
      TCCGCATGTCCAAAGTGCGTACACACGACGTCGAGGGTAAAGTAGTCGTTCAAACCTTCT

6181  accccacatttttcctgcgcgtcatctctgacacggcctccctctgctactccatcctga 6240
      TGGGGTGTAAAAAGGACGCGCAGTAGAGACTGTGCCGGAGGGAGACGATGAGGTAGGACT

6241  aagccaagaacgcagggatgtcgctgggggccaagggcgccgccggccctctgccctccg 6300
      TTCGGTTCTTGCGTCCCTACAGCGACCCCCGGTTCCCGCGGCGGCCGGGAGACGGGAGGC

6301  aggccgtgcagtggctgtgccaccaagcattcctgctcaagctgactcgacaccgtgtca 6360
      TCCGGCACGTCACCGACACGGTGGTTCGTAAGGACGAGTTCGACTGAGCTGTGGCACAGT

6361  cctacgtgccactcctggggtcactcaggacagcccagacgcagctgagtcggaagctcc 6420
      GGATGCACGGTGAGGACCCCAGTGAGTCCTGTCGGGTCTGCGTCGACTCAGCCTTCGAGG

6421  cggggacgacgctgactgccctggaggccgcagccaacccggcactgccctcagacttca 6480
      GCCCCTGCTGCGACTGACGGGACCTCCGGCGTCGGTTGGGCCGTGACGGGAGTCTGAAGT

                                                             NotI 
                                                             |
6481  agaccatcctggactgCCCAACTTTCTTGTACAAAGTGGTTgatatcgggcccgcggccg 6540
      TCTGGTAGGACCTGACGGGTTGAAAGAACATGTTTCACCAACTATAGCCCGGGCGCCGGC

6541  ctggatcctctagcgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatc 6600
      GACCTAGGAGATCGCTCCCGTCTCCTTCAGAAGATTGTACGCCACTGCACCTCCTCTTAG

6601  ccggccctATGGCTACCTCTCGATATGAGCCAGTGGCTGAAATTGGTGTCGGTGCCTATG 6660
      GGCCGGGATACCGATGGAGAGCTATACTCGGTCACCGACTTTAACCACAGCCACGGATAC

6661  GGACAGTGTACAAGGCCCGTGATCCCCACAGTGGCCACTTTGTGGCCCTCAAGAGTGTGA 6720
      CCTGTCACATGTTCCGGGCACTAGGGGTGTCACCGGTGAAACACCGGGAGTTCTCACACT

6721  GAGTCCCCAATGGAGGAGGAGGTGGAGGAGGCCTTCCCATCAGCACAGTTCGTGAGGTGG 6780
      CTCAGGGGTTACCTCCTCCTCCACCTCCTCCGGAAGGGTAGTCGTGTCAAGCACTCCACC

6781  CTTTACTGAGGCGACTGGAGGCTTTTGAGCATCCCAATGTTGTCCGGCTGATGGACGTCT 6840
      GAAATGACTCCGCTGACCTCCGAAAACTCGTAGGGTTACAACAGGCCGACTACCTGCAGA

6841  GTGCCACATCCCGAACTGACCGGGAGATCAAGGTAACCCTGGTGTTTGAGCATGTAGACC 6900
      CACGGTGTAGGGCTTGACTGGCCCTCTAGTTCCATTGGGACCACAAACTCGTACATCTGG

6901  AGGACCTAAGGACATATCTGGACAAGGCACCCCCACCAGGCTTGCCAGCCGAAACGATCA 6960
      TCCTGGATTCCTGTATAGACCTGTTCCGTGGGGGTGGTCCGAACGGTCGGCTTTGCTAGT

6961  AGGATCTGATGCGCCAGTTTCTAAGAGGCCTAGATTTCCTTCATGCCAATTGCATCGTTC 7020
      TCCTAGACTACGCGGTCAAAGATTCTCCGGATCTAAAGGAAGTACGGTTAACGTAGCAAG

7021  ACCGAGATCTGAAGCCAGAGAACATTCTGGTGACAAGTGGTGGAACAGTCAAGCTGGCTG 7080
      TGGCTCTAGACTTCGGTCTCTTGTAAGACCACTGTTCACCACCTTGTCAGTTCGACCGAC

7081  ACTTTGGCCTGGCCAGAATCTACAGCTACCAGATGGCACTTACACCCGTGGTTGTTACAC 7140
      TGAAACCGGACCGGTCTTAGATGTCGATGGTCTACCGTGAATGTGGGCACCAACAATGTG

7141  TCTGGTACCGAGCTCCCGAAGTTCTTCTGCAGTCCACATATGCAACACCTGTGGACATGT 7200
      AGACCATGGCTCGAGGGCTTCAAGAAGACGTCAGGTGTATACGTTGTGGACACCTGTACA

7201  GGAGTGTTGGCTGTATCTTTGCAGAGATGTTTCGTCGAAAGCCTCTCTTCTGTGGAAACT 7260
      CCTCACAACCGACATAGAAACGTCTCTACAAAGCAGCTTTCGGAGAGAAGACACCTTTGA

7261  CTGAAGCCGACCAGTTGGGCAAAATCTTTGACCTGATTGGGCTGCCTCCAGAGGATGACT 7320
      GACTTCGGCTGGTCAACCCGTTTTAGAAACTGGACTAACCCGACGGAGGTCTCCTACTGA

7321  GGCCTCGAGATGTATCCCTGCCCCGTGGAGCCTTTCCCCCCAGAGGGCCCCGCCCAGTGC 7380
      CCGGAGCTCTACATAGGGACGGGGCACCTCGGAAAGGGGGGTCTCCCGGGGCGGGTCACG

7381  AGTCGGTGGTACCTGAGATGGAGGAGTCGGGAGCACAGCTGCTGCTGGAAATGCTGACTT 7440
      TCAGCCACCATGGACTCTACCTCCTCAGCCCTCGTGTCGACGACGACCTTTACGACTGAA

7441  TTAACCCACACAAGCGAATCTCTGCCTTTCGAGCTCTGCAGCACTCTTATCTACATAAGG 7500
      AATTGGGTGTGTTCGCTTAGAGACGGAAAGCTCGAGACGTCGTGAGAATAGATGTATTCC

7501  ATGAAGGTAATCCGGAGTGActcgagtacccatacgacgtcccagactacgcttgagttt 7560
      TACTTCCATTAGGCCTCACTGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAA

                SV40 prom(7571,7839)>>> 
                |
7561  aaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatg 7620
      TTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTAC

7621  catctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagt 7680
      GTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCA

                                                               SV40 origin(7738,7815)>>> 
                                                               |
7681  atgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatc 7740
      TACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAG

7741  ccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatttttttt 7800
      GGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAA

                               SfiI 
                               |
          ORF_2 rf(2)(7805,8473)>>> 
          |                    |
7801  atttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggc 7860
      TAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCG

                   puro marker(7874,8473)>>> 
                   |
7861  ttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgt 7920
      AAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCA

7921  ccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacac 7980
      GGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTG

7981  cgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcg 8040
      GCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGC

8041  cgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctg 8100
      GCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGAC

8101  gaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggc 8160
      CTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCG

8161  cgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggcgccgca 8220
      GCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGT

8221  ccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccaggg 8280
      GGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCC

8281  caagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggt 8340
      GTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCA

8341  gcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggctt 8400
      CGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAA

8401  caccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaa 8460
      GTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTT

8461  gcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaagatt 8520
      CGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAA

8521  gactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcc 8580
      CTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGG

8581  tttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctg 8640
      AAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGAC

8641  gttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcac 8700
      CAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTG

8701  tgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttc 8760
      ACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAG

8761  cgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgc 8820
      GCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACG

8821  ccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaa 8880
      GGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTT

8881  gctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtc 8940
      CGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAG

8941  cttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgcc 9000
      GAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGG

9001  ggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgg 9060
      CCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACC

                            delta_U3 other(9083,9135)>>> 
                            |
9061  gccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctg 9120
      CGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGAC

                     HIV-1_5_LTR other(9136,9316)>>> 
                     |
9121  ctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggc 9180
      GAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCG

9181  taactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtg 9240
      ATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCAC

9241  tgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtg 9300
      ACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCAC

9301  tggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgca 9360
      ACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGT

9361  aagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaa 9420
      TTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTT

9421  taaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgt 9480
      ATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACA

9481  ggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggaga 9540
      CCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCT

9541  aagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacga 9600
      TTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCT

9601  tcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttcc 9660
      AGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGG

9661  gcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagct 9720
      CGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGA

9721  cactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatg 9780
      GTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTAC

                                               pBR322 origin(9822,10441)<<< 
                                               |
9781  tgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttc 9840
      ACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAG

9841  cataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcga 9900
      GTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCT

9901  aacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctct 9960
      TTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGA

9961  cctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtg 10020
      GGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCAC

10021 gcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaag 10080
      CGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTC

10081 ctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactat 10140
      GACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATA

10141 cgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaac 10200
      GCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTG

10201 aggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaac 10260
      TCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTG

10261 tacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttc 10320
      ATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAG

10321 ggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttt 10380
      CCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAA

10381 tttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatc 10440
      AAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAG

10441 ttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatg 10500
      AAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTAC

10501 agattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatca 10560
      TCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGT

10561 atctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca 10620
      TAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGT

10621 cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtag 10680
      GGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATC

10681 ataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagac 10740
      TATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTG

10741 ccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgc 10800
      GGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCG

10801 agaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagct 10860
      TCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGA

10861 agagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacct 10920
      TCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGA

10921 agatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtca 10980
      TCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGT

10981 gctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgca 11040
      CGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGT

                                                           NEOKAN prom(11094,11143)>>> 
                                                           |
11041 gtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaat 11100
      CACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTA

11101 tgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctt 11160
      ACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAA

                                               BclI 
                                               |
11161 tctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgag 11220
      AGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTC

                    NTP_II marker(11235,12023)>>> 
                    |
                 ORF_4 rf(3)(11232,12026)>>> 
                 |  |
11221 gatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtgg 11280
      CTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACC

11281 agaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgt 11340
      TCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACA

11341 tccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccc 11400
      AGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGG

11401 tgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttcctt 11460
      ACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAA

11461 gcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaag 11520
      CGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTC

11521 tgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatgg 11580
      ACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACC

11581 ctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaag 11640
      GACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTC

11641 cgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatg 11700
      GCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTAC

11701 atctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcga 11760
      TAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCT

11761 gcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatca 11820
      CGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGT

11821 tggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggacc 11880
      ACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGG

11881 gctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatggg 11940
      CGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCC

11941 ctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttct 12000
      GACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGA

12001 atcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattt 12060
      TAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAA

12061 tttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgata 12120
      AAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTAT

                f1 origin(12131,12436)<<< 
                |
12121 gggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaac 12180
      CCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTG

12181 gtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaa 12240
      CAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTT

12241 tcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccc 12300
      AGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGG

12301 cgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcg 12360
      GCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGC

12361 aaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccaca 12420
      TTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGT

12421 cccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaat 12480
      GGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTA

12481 tcttaattaacatcatcaataatatacctt 12510
      AGAATTAATTGTAGTAGTTATTATATGGAA

                            LOCUS       dna                     12510 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     Promoter        2885..2966
                     /gene="CMV prom"
     ORF             2901..7520
                     /sequence="ORF_3 rf(3)"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     ORF             3072..3713
                     /sequence="ORF_6 rf(5)"
     ORF             4134..5354
                     /sequence="ORF_5 rf(5)"
     misc_binding    6534..6541
                     /dbxref="REBASE:NotI"
     Promoter        7571..7839
                     /gene="SV40 prom"
     Rep_Origin      7738..7815
                     /gene="SV40 origin"
     ORF             7805..8473
                     /sequence="ORF_2 rf(2)"
     misc_binding    7818..7830
                     /dbxref="REBASE:SfiI"
     Marker          7874..8473
                     /gene="puro marker"
     Other Gene      9083..9135
                     /gene="delta_U3 other"
     Other Gene      9136..9316
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      9822..10441
                     /gene="pBR322 origin"
     Promoter        11094..11143
                     /gene="NEOKAN prom"
     misc_binding    11201..11206
                     /dbxref="REBASE:BclI"
     ORF             11232..12026
                     /sequence="ORF_4 rf(3)"
     Marker          11235..12023
                     /gene="NTP_II marker"
     Rep_Origin      12131..12436
                     /gene="f1 origin"
BASE COUNT    2823 a   3408 c   3559 g   2720 t    0 others